LOCUS       sequence-374682-        8727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sgRNA vector backbone expressing puromycin resistance and CD90.1 as 
            selection markers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Turner DJ, Saveliev A, Salerno F, Matheson LS, Screen M, Lawson H, 
            Wotherspoon D, Kranc KR, Turner M
  TITLE     A functional screen of RNA binding proteins identifies genes that 
            promote or limit the accumulation of CD138+ plasma cells.
  JOURNAL   eLife. 2022 Apr 22;11. pii: 72313. doi: 10.7554/eLife.72313.
  PUBMED    35451955
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2022-04-22"; volume: "11. pii: 72313. doi"; pages: " 
            10.7554/eLife.72313"
COMMENT     Sequence Label: 
            MSCV_hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-Thy1.1
FEATURES             Location/Qualifiers
     source          1..8727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1472..1720
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1472..1492
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1643..1662
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     mobile_element  1863..2630
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             2852..3157
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3036..3055
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     promoter        3318..3817
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3353..3376)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3750..3769
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3857..4453
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3857..3876)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4353..4373
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4472..4525
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4565..5053
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     LTR             5120..5634
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5796..5812
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5820..5826,5827..5844,5845..5850))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5865..5886
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6003..6020)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6174..6762)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6254..6273)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6933..7724,7725..7793))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7556..7575
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7794..7898)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7966..7984
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8022..8044)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8144..8163
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8357..8379
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8507..8526
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(8726..8727,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctctcga ggatccagtt tggttagtac
     1441 cgggccctac gcgtccaagg tcgggcagga agagggccta tttcccatga ttccttcata
     1501 tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac
     1561 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
     1621 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
     1681 tttcttggct ttatatatct tgtggaaagg acgaaacacc gggtcttccg taatctagag
     1741 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
     1801 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     1861 gcggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg
     1921 gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc
     1981 aaaagcaccg ccggacatca gcgctatctc tgctctcact gccgtaaaac atggcaactg
     2041 cagttcactt acaccgcttc tcaacccggt acgcaccaga aaatcattga tatggccatg
     2101 aatggcgttg gatgccgggc aacagcccgc attatgggcg ttggcctcaa cacgatttta
     2161 cgtcacttaa aaaactcagg ccgcagtcgg taacctcgcg catacagccg ggcagtgacg
     2221 tcatcgtctg cgcggaaatg gacgaacagt ggggctatgt cggggctaaa tcgcgccagc
     2281 gctggctgtt ttacgcgtat gacagtctcc ggaagacggt tgttgcgcac gtattcggtg
     2341 aacgcactat ggcgacgctg gggcgtctta tgagcctgct gtcacccttt gacgtggtga
     2401 tatggatgac ggatggctgg ccgctgtatg aatcccgcct gaagggaaag ctgcacgtaa
     2461 tcagcaagcg atatacgcag cgaattgagc ggcataacct gaatctgagg cagcacctgg
     2521 cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
     2581 tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc tgaagcagcg
     2641 tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat
     2701 atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc
     2761 tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc
     2821 tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag
     2881 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg
     2941 acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtcc cccgtgaact
     3001 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag
     3061 tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat
     3121 caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag
     3181 ccagtctgca ggtcgaccat agaagacacg tttaagagct atgctggaaa cagcatagca
     3241 agtttaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     3301 ttggatccaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     3361 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     3421 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     3481 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     3541 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     3601 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     3661 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     3721 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     3781 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcatccatc tagatctcga
     3841 gcagctgaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     3901 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     3961 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     4021 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     4081 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     4141 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     4201 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     4261 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     4321 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     4381 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     4441 caagcccggt gccggcggcg ggtccggagg agagggcaga ggaagtctcc taacatgcgg
     4501 tgacgtggag gagaatcctg gcccaaccat gggtcttttc tgcagtcacc gtcctcgagg
     4561 caccatgaac ccagccatca gcgtcgctct cctgctctca gtcttgcagg tgtcccgagg
     4621 gcagaaggtg accagcctga cagcctgcct ggtgaaccaa aaccttcgcc tggactgccg
     4681 ccatgagaat aacaccaagg ataactccat ccagcatgag ttcagcctga cccgagagaa
     4741 gaggaagcac gtgctctcag gcacccttgg gatacccgag cacacgtacc gctcccgcgt
     4801 caccctctcc aaccagccct atatcaaggt ccttacccta gccaacttca ccaccaagga
     4861 tgagggcgac tacttttgtg agcttcgcgt ctcgggcgcg aatcccatga gctccaataa
     4921 aagtatcagt gtgtatagag acaagctggt caagtgtggc ggcataagcc tgctggttca
     4981 gaacacatcc tggatgctgc tgctgctgct ttccctctcc ctcctccaag ccctggactt
     5041 catttctctg tgagtcgacc tgcagccaag cttatcgata aaataaaaga ttttatttag
     5101 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     5161 aacgccattt tgcaaggcat ggaaaataca taactgagaa tagagaagtt cagatcaagg
     5221 ttaggaacag agagacagca gaatatgggc caaacaggat atctgtggta agcagttcct
     5281 gccccggctc agggccaaga acagatggtc cccagatgcg gtcccgccct cagcagtttc
     5341 tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat
     5401 ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc
     5461 aataaaagag cccacaaccc ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc
     5521 gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt
     5581 ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatgggtaa
     5641 cagtttcttg aagttggaga acaacattct gagggtagga gtcgaatatt aagtaatcct
     5701 gactcaatta gccactgttt tgaatccaca tactccaata ctcctgaaat agttcattat
     5761 ggacagcgca gaaagagctg gggagaattg tgaaattgtt atccgctcac aattccacac
     5821 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     5881 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     5941 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6001 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6061 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6121 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6181 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6241 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6301 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6361 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6421 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6481 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6541 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6601 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6661 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6721 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6781 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6841 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6901 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6961 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7021 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7081 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7141 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7201 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7261 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7321 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7381 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7441 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7501 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7561 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7621 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7681 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7741 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7801 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7861 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7921 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     7981 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     8041 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     8101 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     8161 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     8221 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     8281 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     8341 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg aatggtgcat
     8401 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     8461 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     8521 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     8581 agaggcgatt agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga
     8641 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt
     8701 tatttagtct ccagaaaaag gggggaa
//
