LOCUS       sequence-37473-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Valastyan JS, Lindquist S
  TITLE     TorsinA and the TorsinA-Interacting Protein Printor Have No Impact 
            on Endoplasmic Reticulum Stress or Protein Trafficking in Yeast.
  JOURNAL   PLoS One. 2011;6(7):e22744. Epub 2011 Jul 26.
  PUBMED    21818381
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(7)"; pages: "e22744. Epub 2011 Jul 26"
COMMENT     Sequence Label: 413-Met25 Kar2 TorsinADE K108A GFP
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        58..407
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     primer_bind     408..424
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     408..424
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    432..456
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcctaaggga acaaagctgg agctccggat gcaagggttc gaatccctta gctctcatta
       61 ttttttgctt tttctcttga ggtcacatga tcgcaaaatg gcaaatggca cgtgaagctg
      121 tcgatattgg ggaactgtgg tggttggcaa atgactaatt aagttagtca aggcgccatc
      181 ctcatgaaaa ctgtgtaaca taataaccga agtgtcgaaa aggtggcacc ttgtccaatt
      241 gaacacgctc gatgaaaaaa ataagatata tataaggtta agtaaagcgt ctgttagaaa
      301 ggaagttttt cctttttctt gctctcttgt cttttcatct actatttcct tcgtgtaata
      361 cagggtcgtc agatacatag atacaattct attaccccca tccatactct agaactagtg
      421 gatcccccat cacaagtttg tacaaaaaag caggcttcac aaaatgtttt tcaacagact
      481 aagcgctggc aagctgctgg taccactctc cgtggtcctg tacgcccttt tcgtggtaat
      541 attaccttta cagaattctt tccactcctc caatgtttta gttagaggtg tggagcccat
      601 cagcctggga ctggccctgg ccggcgtcct caccggctac atctacccgc gtctctactg
      661 cctcttcgcc gagtgctgcg ggcagaagcg gagccttagc cgggaggcac tgcagaagga
      721 tctggacgac aacctctttg gacagcatct tgcaaagaaa atcatcttaa atgccgtgtt
      781 tggtttcata aacaacccaa agcccaagaa acctctcacg
//
