LOCUS       sequence-37474-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Subach FV, Subach OM, Gundorov IS, Morozova KS, Piatkevich KD, 
            Cuervo AM, Verkhusha VV
  TITLE     Monomeric fluorescent timers that change color from blue to red 
            report on cellular trafficking.
  JOURNAL   Nat Chem Biol. 2009 Feb;5(2):118-26. Epub 2009 Jan 11.
  PUBMED    19136976
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol."; date: "2009-02"; volume: "5(2)"; pages: "118-26. Epub 2009 
            Jan 11"
COMMENT     Sequence Label: pMedium-FT-N1
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    12..92
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      94..103
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             100..810
                     /codon_start=1
                     /product="blue-to-red fluorescent timer derivative of 
                     mCherry (Subach et al., 2009)"
                     /label=Medium-FT
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHLEGSVDGHEFEIEGEGEGRPYEG
                     TQSAKLKVTKGGPLPFAWDILSPQFMYGSRAYVKHPADIPDYWKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERIYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVLLPGAYNVNIKMDITSHNEDYTIVEQCERA
                     EGHHSTGGMDELYK"
     primer_bind     complement(245..263)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     513..532
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag
       61 tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag
      121 gataacatgg ccatcatcaa ggaattcatg cgtttcaagg tgcacctgga gggctccgtg
      181 gacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
      241 agcgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
      301 cctcagttca tgtacggctc cagggcctac gtgaagcacc ccgccgacat ccccgactac
      361 tggaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggatggc
      421 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
      481 aagctgcgcg gcaccaactt cccttccgac ggccccgtaa tgcagaagaa gaccatgggc
      541 tgggaggcct cctccgagcg gatatacccc gaggacggcg ccctgaaggg cgagatcaag
      601 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
      661 gccaagaagc ccgtgctgct gcccggcgcc tacaacgtca acatcaagat ggacatcacc
      721 tcccacaacg aggactacac catcgttgaa cagtgcgaac gcgccgaggg ccaccattcc
      781 accggcggca tggacgagct gtacaagtaa gcggccgcga ctctagatca taatcagcca
      841 taccaca
//
