LOCUS       sequence-374747-        7372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express pmRFP-PAGFP-RhoA Q63L .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Budnar S, Husain KB, Gomez GA, Naghibosadat M, Varma A, Verma S, 
            Hamilton NA, Morris RG, Yap AS
  TITLE     Anillin Promotes Cell Contractility by Cyclic Resetting of RhoA 
            Residence Kinetics
  JOURNAL   Dev Cell. 2019 Jun 17;49(6):894-906.e12. doi: 
            10.1016/j.devcel.2019.04.031. Epub 2019 May 16.
  PUBMED    31105010
REFERENCE   2  (bases 1 to 7372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2019.04.031"; journalName: "Dev Cell"; date: 
            "2019-06-17- 17"; volume: "49"; issue: "6"; pages: "894-906.e12"
COMMENT     Sequence Label: mRFP-PA-GFP-RhoAQ63L
FEATURES             Location/Qualifiers
     source          1..7372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     380..399
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(618..722)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(797..816)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        988..1367
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1368..1571
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1522..1542
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1616..1635
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1616..1634
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1650..2324
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             1650..2324
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(1780..1798)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2048..2067
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2242..2265
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             join(2346..2348,2349..2351,2352..3062)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2391..2412)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2652..2671)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2999..3020
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             join(3069..3254,3255..3257,3258..3650)
                     /codon_start=1
                     /product="constitutively active mutant of human Rho family 
                     small GTPase RhoA"
                     /label=RhoA (Q63L)
                     /translation="MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGLEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
     promoter        complement(3678..3696)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3679..3696)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3716..3733)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3722..3946
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3992..4420
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4079..4098)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4289..4310
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4429..4449)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4434..4763
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4614..4749
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4676..4695
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4830..5624
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4884..4903)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5494..5513
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5798..5919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5835..5854)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5889..5908
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5968..5984)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5968..5984)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5981..6003)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5992..6008
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6016..6022,6023..6040,6041..6046))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6061..6082
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6199..6216)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6370..6958)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6450..6469)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7129..7372,1..548,549..617))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
       61 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
      121 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
      181 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
      241 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
      301 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
      361 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
      421 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
      481 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      541 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
      601 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
      661 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
      721 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
      781 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
      841 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
      901 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
      961 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
     1021 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     1081 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1141 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1201 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1261 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1321 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1381 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1441 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     1501 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1561 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
     1621 cgactcacta tagggagacc caagctagca tggcctcctc cgaggacgtc atcaaggagt
     1681 tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     1741 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     1801 gcggccccct gcccttcgcc tgggacatcc tgtcccctca gttccagtac ggctccaagg
     1861 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     1921 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     1981 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     2041 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcggatgt
     2101 accccgagga cggcgccctg aagggcgaga tcaagatgag gctgaagctg aaggacggcg
     2161 gccactacga cgccgaggtc aagaccacct acatggccaa gaagcccgtg cagctgcccg
     2221 gcgcctacaa gaccgacatc aagctggaca tcacctccca caacgaggac tacaccatcg
     2281 tggaacagta cgagcgcgcc gagggccgcc actccaccgg cgcctccgga ctcagatctc
     2341 gaggcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     2401 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     2461 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     2521 ccaccctcgt gaccaccttc agctacggcg tgcagtgctt cagccgctac cccgaccaca
     2581 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     2641 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     2701 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     2761 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     2821 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     2881 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     2941 accactacct gagccaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     3001 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     3061 aggaattcat ggctgccatc cggaagaaac tggtgattgt tggtgatgga gcctgtggaa
     3121 agacatgctt gctcatagtc ttcagcaagg accagttccc agaggtgtat gtgcccacag
     3181 tgtttgagaa ctatgtggca gatatcgagg tggatggaaa gcaggtagag ttggctttgt
     3241 gggacacagc tgggctggaa gattatgatc gcctgaggcc cctctcctac ccagataccg
     3301 atgttatact gatgtgtttt tccatcgaca gccctgatag tttagaaaac atcccagaaa
     3361 agtggacccc agaagtcaag catttctgtc ccaacgtgcc catcatcctg gttgggaata
     3421 agaaggatct tcggaatgat gagcacacaa ggcgggagct agccaagatg aagcaggagc
     3481 cggtgaaacc tgaagaaggc agagatatgg caaacaggat tggcgctttt gggtacatgg
     3541 agtgttcagc aaagaccaaa gatggagtga gagaggtttt tgaaatggct acgagagctg
     3601 ctctgcaagc tagacgtggg aagaaaaaat ctggttgcct tgtcttgtga ctcgagcatg
     3661 catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg
     3721 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3781 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3841 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     3901 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     3961 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     4021 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4081 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4141 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4201 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4261 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4321 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4381 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     4441 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4501 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4561 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4621 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4681 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     4741 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     4801 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     4861 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     4921 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     4981 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     5041 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     5101 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     5161 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     5221 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     5281 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     5341 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     5401 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     5461 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     5521 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     5581 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     5641 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     5701 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     5761 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     5821 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5881 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     5941 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6001 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6061 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6121 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6181 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6241 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6301 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6361 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6421 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6481 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6541 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6601 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6661 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6721 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6781 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6841 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6901 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6961 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7021 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7081 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7141 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7201 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7261 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7321 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ct
//
