LOCUS       sequence-374748-        9180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of trimeric G protein with mCherry2 (GNB1 and GNG2) or 
            mEYFP(Q69K) (GNAI1) tags..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Foust DJ, Piston DW
  TITLE     Measuring G Protein Activation with Spectrally Resolved Fluorescence
            Fluctuation Spectroscopy
  JOURNAL   bioRxiv 2021.11.03.467169
REFERENCE   2  (bases 1 to 9180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.11.03.467169"
COMMENT     Sequence Label: 
            pmCherry2-GNB1-T2A-mCherry2-GNG2-IRES-GNAI1-mEYFP(Q69K)
FEATURES             Location/Qualifiers
     source          1..9180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             613..1320
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             2395..2448
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2455..3162
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3462..3479)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    3491..4064
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3514..4066
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3658..3675)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3885..3904
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4438..4440,4441..4443,4444..4956)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(4483..4504)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4744..4763)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5091..5112
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5968..6089
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6005..6024)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6059..6078
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6096..6551)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6233..6254)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6445..6464
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6578..6682
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6684..7041
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6684..6875
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6707..6727)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6892..7027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7076..7870
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7076..7870
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7130..7149)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7740..7759
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8058..8077)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8102..8149
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8478..9066
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8967..8986
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aattgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagtg tcgtaccatg ggctgggagg cctccactga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcgacatcaa gttggacatc ctttcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagc tcaagcttcg aattcactag tgattgccgc caccatgagt
     1381 gagcttgacc agttacggca ggaggccgag caacttaaga accagattcg agacgccagg
     1441 aaagcatgtg cagatgcaac tctctctcag atcacaaaca acatcgaccc agtgggaaga
     1501 atccaaatgc gcacgaggag gacactgcgg gggcacctgg ccaagatcta cgccatgcac
     1561 tggggcacag actccaggct tctcgtcagt gcctcgcagg atggtaaact tatcatctgg
     1621 gacagctaca ccaccaacaa ggtccacgcc atccctctgc gctcctcctg ggtcatgacc
     1681 tgtgcatatg ccccttctgg gaactatgtg gcctgcggtg gcctggataa catttgctcc
     1741 atttacaatc tgaaaactcg tgaggggaac gtgcgcgtga gtcgtgagct ggcaggacac
     1801 acaggttacc tgtcctgctg ccgattcctg gatgacaatc agatcgtcac cagctctgga
     1861 gacaccacgt gtgccctgtg ggacatcgag accggccagc agacgaccac gtttaccgga
     1921 cacactggag atgtcatgag cctttctctt gctcctgaca ccagactgtt cgtctctggt
     1981 gcttgtgatg cttcagccaa actctgggat gtgcgagaag gcatgtgccg gcagaccttc
     2041 actggccacg agtctgacat caatgccatt tgcttctttc caaatggcaa tgcatttgcc
     2101 actggctcag acgacgccac ctgcaggctg tttgaccttc gtgctgacca ggagctcatg
     2161 acttactccc atgacaacat catctgcggg atcacctctg tctccttctc caagagcggg
     2221 cgcctcctcc ttgctgggta cgacgacttc aactgcaacg tctgggatgc actcaaagcc
     2281 gaccgggcag gtgtcttggc tgggcatgac aaccgcgtca gctgcctggg cgtgactgac
     2341 gatggcatgg ctgtggcgac agggtcctgg gatagcttcc tcaagatctg gaacgagggc
     2401 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctac cggtatggtg
     2461 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     2521 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     2581 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     2641 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     2701 gacatccccg actacttgaa gctgtccttc cccgagggct tcaattggga gcgcgtgatg
     2761 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     2821 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     2881 tgtcgtacca tgggctggga ggcctccact gagcggatgt accccgagga cggcgccctg
     2941 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     3001 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcgacatc
     3061 aagttggaca tcctttccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     3121 gagggccgcc actccaccgg cggcatggac gagctgtaca agtccggact cagatctatg
     3181 gccagcaaca acaccgccag catagcacaa gccaggaagc tggtagagca gcttaagatg
     3241 gaagccaata tcgacaggat aaaggtgtcc aaggcagctg cagatttgat ggcctactgt
     3301 gaagcacatg ccaaggaaga ccccctcctg acccctgttc cggcttcaga aaacccgttt
     3361 agggagaaga agtttttctg tgccatcctt taaccgcggc gtacgaggcc tgcatgctcc
     3421 ggacctgcag gttcgaagtc gacagatctc aattggggcc cctatagtgt cacctaaata
     3481 attccgcccc cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     3541 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     3601 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     3661 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     3721 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     3781 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     3841 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     3901 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     3961 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     4021 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggg ctgcacactg
     4081 agcgctgagg acaaggcggc cgtggagcgc agcaagatga tcgaccgcaa cctccgggag
     4141 gacggagaga aggcagcgcg cgaggtcaag ctgctgctgc tgggtgctgg tgaatccggg
     4201 aagagcacaa ttgtgaagca gatgaaaatt atccacgagg ctggctactc agaggaagag
     4261 tgtaagcagt acaaagcagt ggtctacagc aacaccatcc agtccatcat tgccatcatt
     4321 agagctatgg ggagattgaa aatcgacttt ggagacgctg ctcgtgcgga tgatgctcgc
     4381 caactcttcg tgcttgctgg ggctgcagag gaaggcttta tgaccgcgac cggttctatg
     4441 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     4501 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     4561 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     4621 gtgaccacct tcggctacgg cctgaagtgc ttcgcccgct accccgacca catgaagcag
     4681 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     4741 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     4801 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     4861 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     4921 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     4981 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     5041 ctgagctacc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     5101 ctggagttcg tgaccgccgc cgggatcact ggtgaactcg ccggcgtcat aaagagactg
     5161 tggaaggaca gcggtgtgca agcctgcttc aacagatccc gggagtacca gctgaacgat
     5221 tcggcggcgt actacctgaa tgacttggac agaatagcac aaccaaatta catcccaacc
     5281 cagcaggatg ttctcagaac tagagtgaaa acgacgggaa ttgtggaaac ccactttact
     5341 ttcaaagatc ttcattttaa aatgtttgac gtgggaggcc agagatcaga gcggaagaag
     5401 tggattcact gctttgaagg cgtgactgcc atcatcttct gtgtggccct gagtgactat
     5461 gacctggttc ttgctgagga tgaagaaatg aaccggatgc acgaaagcat gaagctgttc
     5521 gatagcatat gtaacaacaa gtggtttacg gacacatcca tcatcctttt cctgaacaag
     5581 aaggacctct tcgaagagaa gatcaaaaag agtcccctca cgatatgcta tccagaatat
     5641 gcaggctcaa acacatatga agaggcggct gcgtatatcc agtgtcagtt tgaagacctc
     5701 aataaaagga aggacacaaa ggaaatttac acccacttca cttgcgccac ggatacgaag
     5761 aatgtgcagt ttgtgttcga tgctgtaacg gacgtcatca taaagaataa cctaaaagac
     5821 tgtggtctct tctaagctgg atccaccgga tctagataac tgatcataat cagccatacc
     5881 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     5941 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     6001 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6061 ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt
     6121 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     6181 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     6241 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     6301 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     6361 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     6421 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     6481 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     6541 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     6601 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6661 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     6721 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6781 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6841 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6901 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6961 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7021 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
     7081 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     7141 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     7201 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     7261 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     7321 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     7381 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     7441 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     7501 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     7561 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     7621 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     7681 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     7741 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     7801 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     7861 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     7921 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     7981 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     8041 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     8101 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     8161 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     8221 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     8281 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     8341 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     8401 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8461 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8521 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8581 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     8641 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8701 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8761 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8821 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8881 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     8941 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9001 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9061 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9121 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
