LOCUS       sequence-37475-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Valastyan JS, Lindquist S
  TITLE     TorsinA and the TorsinA-Interacting Protein Printor Have No Impact 
            on Endoplasmic Reticulum Stress or Protein Trafficking in Yeast.
  JOURNAL   PLoS One. 2011;6(7):e22744. Epub 2011 Jul 26.
  PUBMED    21818381
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(7)"; pages: "e22744. Epub 2011 Jul 26"
COMMENT     Sequence Label: 413-Met25 Kar2 TorsinA E171Q GFP
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        59..408
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     primer_bind     409..425
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     409..425
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    433..457
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctcctaaggg acaaaagctg gagctccgga tgcaagggtt cgaatccctt agctctcatt
       61 attttttgct ttttctcttg aggtcacatg atcgcaaaat ggcaaatggc acgtgaagct
      121 gtcgatattg gggaactgtg gtggttggca aatgactaat taagttagtc aaggcgccat
      181 cctcatgaaa actgtgtaac ataataaccg aagtgtcgaa aaggtggcac cttgtccaat
      241 tgaacacgct cgatgaaaaa aataagatat atataaggtt aagtaaagcg tctgttagaa
      301 aggaagtttt tcctttttct tgctctcttg tcttttcatc tactatttcc ttcgtgtaat
      361 acagggtcgt cagatacata gatacaattc tattaccccc atccatactc tagaactagt
      421 ggatccccca tcacaagttt gtacaaaaaa gcaggcttca caaaatgttt ttcaacagac
      481 taagcgctgg caagctgctg gtaccactct ccgtggtcct gtacgccctt ttcgtggtaa
      541 tattaccttt acagaattct ttccactcct ccaatgtttt agttagaggt gtggagccca
      601 tcagcctggg actggccctg gccggcgtcc tcaccggcta catctacccg cgtctctact
      661 gcctcttcgc cgagtgctgc gggcagaagc ggagccttag ccgggaggca ctgcagaagg
      721 atctggacga caacctcttt ggacagcatc ttgcaaagaa aatcatctta aatgccgtgt
      781 ttggtttcat aaacaaccca aagcccaaga aacctctcac gctctccctg cacgggtgga
      841 caggcaccgg caaaaatttc gtcagcaaga tcatcgcaaa
//
