LOCUS       sequence-374754-        8984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express RPS9 WT mouse gene transcriptionally fused with eGFP. eGFP 
            is translated from IRES..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8984)
  AUTHORS   Brilkova M, Nigri M, Kumar HS, Moore J, Mantovani M, Keller C, Grimm
            A, Eckert A, Shcherbakov D, Akbergenov R, Seebeck P, Kramer SD, 
            Wolfer DP, Gent TC, Bottger EC
  TITLE     Error-prone protein synthesis recapitulates early symptoms of 
            Alzheimer disease in aging mice.
  JOURNAL   Cell Rep. 2022 Sep 27;40(13):111433. doi: 
            10.1016/j.celrep.2022.111433.
  PUBMED    36170830
REFERENCE   2  (bases 1 to 8984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-09-27"; volume: "40(13):111433. doi"; pages: " 
            10.1016/j.celrep.2022.111433"
COMMENT     Sequence Label: pRps9-WT-IRES-GFP-HygR
FEATURES             Location/Qualifiers
     source          1..8984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        62..441
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        443..719
                     /label=chicken beta-actin promoter
     intron          720..1736
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1660..1682
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1744..1763
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    join(2469..3043,3044..3046,3047..3055)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2636..2653)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2863..2882
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3056..3058,3059..3061,3062..3775)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLANRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3101..3122)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3362..3381)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3709..3730
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3814..4436
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4013..4032)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     polyA_signal    4523..4571
                     /note="synthetic polyadenylation signal"
     CDS             complement(4595..5632)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             4608..4616
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(5663..6020)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5677..5812
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5731..5750)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        5829..6020
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5977..5997
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(6144..6164)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6144..6162)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6183..6199)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6183..6199)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6196..6218)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6207..6223
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6231..6237,6238..6255,6256..6261))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6276..6297
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6414..6431)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6585..7173)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6665..6684)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7344..8135,8136..8204))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7967..7986
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8205..8309)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8335..8790)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8472..8493)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8684..8703
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8917..8939
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8931..8948
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8932..8948
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8958..8977
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8958..8976
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgcgcca gcccgggctg gccggcctgc gatcgctgat atcgtcgcga cgaattcact
       61 cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
      121 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      181 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      241 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      301 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      361 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      421 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
      481 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      541 atgggggcgg gggggggggg gggcgcgcgc caggsggggs ggggsggggs grggggsggg
      601 gsggggsgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      661 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      721 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      781 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      841 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      901 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      961 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
     1021 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1081 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1141 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1201 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1261 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1321 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1381 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1441 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1501 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1561 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1621 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1681 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1741 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc accgatcaga
     1801 agcttcgtta actgagctca ggatcctcct aggataccat gccggtcgcc agaagctggg
     1861 tttgtcgcaa aacctatgtg accccacgga gacccttcga gaagtcgcgt ctcgaccagg
     1921 agctaaagtt gattggagag tatggactcc ggaacaaacg tgaggtttgg agggtcaagt
     1981 ttaccctggc caagatccgt aaggcggccc gggagctgtt gacgctagac gagaaggatc
     2041 cccggcgtct gtttgaaggc aatgctctcc tgcggcggct tgttcgcatt ggggtgctgg
     2101 acgagggcaa gatgaagctg gattacatcc tgggcctgaa gattgaggat ttcttggaga
     2161 ggcggctgca gacccaggtc tttaagctgg gcctggccaa atctattcac catgcccgtg
     2221 tgctcatccg ccaacgtcac attagggtcc gcaagcaggt ggtgaacatc ccatccttca
     2281 ttgttcgcct ggactctcag aagcacatcg acttctccct ccgttctcct tatggcggcg
     2341 gccgtccagg ccgagtgaag aggaagaatg ccaagaaagg ccagggcggg gctggagctg
     2401 gtgatgatga ggaagaggat taactcgagg gccggcgcgc cgcggccgct acgtaaattc
     2461 cgcccccccc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     2521 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     2581 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     2641 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     2701 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     2761 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     2821 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     2881 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     2941 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     3001 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt
     3061 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     3121 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     3181 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     3241 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     3301 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     3361 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggcgaa
     3421 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     3481 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     3541 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     3601 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     3661 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     3721 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgc
     3781 ctgtacagtg gtcgacgatt cgataagctt gatgggtggc atccctgtga cccctcccca
     3841 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa
     3901 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg
     3961 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg
     4021 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt
     4081 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
     4141 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc
     4201 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag
     4261 gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa aataactata ccagcaggag
     4321 gacgtccaga cacagcatag gctacctggc catgcccaac cggtgggaca tttgagttgc
     4381 ttgcttggca ctgtcctctc atgcgttggg tccactcagt agatgcctgt tgaattcctg
     4441 cagcccccta cctgcaggta cgcgtaggag ctgactgggt tgaaggctct caagggcatc
     4501 ggtcgacagc atctagttcg aacacacaaa aaaccaacac acagatgtaa tgaaaataaa
     4561 gatattttat tgcggccatg accggttcta gagtttaaac tcgacctacc tccttagcgc
     4621 gcggtcgtgt actgggccgg cggttgccgc tgtcggccag cacctcgacg cagccgtcgg
     4681 tccatacggc tgcgctccgg cgagcgattt gtgtgcgacc gacggtgccg gccccgctgc
     4741 ggacgatggc atcgcagcgg ccttgagccc aggcagcatc gtcgaagttg ccgtccacga
     4801 ggctctggta gagctgatcc aggccgatgc gcagcatgta ggctcgcaga cgagggctgc
     4861 cggccagctc gggatgccgg cgctcgaagt agcgagtctg ctgctccatg caagccagcc
     4921 agggccgcca gaagaagatg ttggccacct cgtactgact gtccccgaac atagcttcgg
     4981 accagtcgat tacggcggtg atgcggccgt tgtcggtcag gacgttgttg ctgccgaagt
     5041 cggcatggac gaggtggcgc acctcgggac agtcttcggc ccacagcatc agttcgtcca
     5101 gggcttgagc tacgctggcg gacacggtgt cgtccatcac ggtctgccag tggtagacat
     5161 ggggatcagc aatggcgcaa atgaaatccc gccaggttgt gtactggccg atgccttggg
     5221 gcccgaatgg gccgaagccg ctggtttgac tgaggtcggc ggcggcaata gcatccatag
     5281 cttcggcgac aggctgtaac acagcaggca gctctgtttc ggggaggtct tggagagtaa
     5341 cgccttgggc gcgtctactg atgcagtatg tcaggctctc gctgaactcg ccgatgtcca
     5401 acacttcggg gatgggtagt gcagcgctgg cgaagtggcg gtacacgtag cggtctttgt
     5461 agaagccatc agcgcagcta ttcacccgca gtacatagcc gcgtccgccg acatcgaagc
     5521 tgaaggctcg gctctcttcg ccctccgaca actgcatcag gtcgctcaca ctgtcgaact
     5581 tctcgatgag aaatttttca acgctggtag cggtgagttc gggcttcttc atggtggcgc
     5641 tagtgtcaga agaatcgagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact
     5701 acttctggaa tagctcagag gcagaggcgg cctcggcctc tgcataaata aaaaaaatta
     5761 gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag
     5821 ttaggggcgg gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
     5881 ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca
     5941 tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac acattccaca
     6001 gctggttctt tccgcctcag aaggtagcta accaagttcc tctttcagag gttatttcag
     6061 gccatggtgc tgcgcagatc agcggaagag cgcccaatac gcgtcgttta aactcacgtg
     6121 gttaattaat ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     6181 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6241 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6301 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6361 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6421 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6481 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6541 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6601 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6661 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6721 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6781 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6841 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6901 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6961 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7021 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7081 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7141 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7201 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7261 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7321 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7381 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7441 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7501 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7561 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7621 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7681 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7741 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7801 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7861 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7921 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7981 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8041 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8101 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8161 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8221 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8281 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
     8341 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     8401 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     8461 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     8521 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     8581 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     8641 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     8701 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     8761 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
     8821 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     8881 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     8941 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attg
//
