LOCUS       sequence-37476-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Valastyan JS, Lindquist S
  TITLE     TorsinA and the TorsinA-Interacting Protein Printor Have No Impact 
            on Endoplasmic Reticulum Stress or Protein Trafficking in Yeast.
  JOURNAL   PLoS One. 2011;6(7):e22744. Epub 2011 Jul 26.
  PUBMED    21818381
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(7)"; pages: "e22744. Epub 2011 Jul 26"
COMMENT     Sequence Label: 413-Met25 Kar2 TorsinADE E171Q GFP
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        60..409
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     primer_bind     410..426
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     410..426
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    434..458
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctcctaaggg aacaaaagct ggagctccgg atgcaagggt tcgaatccct tagctctcat
       61 tattttttgc tttttctctt gaggtcacat gatcgcaaaa tggcaaatgg cacgtgaagc
      121 tgtcgatatt ggggaactgt ggtggttggc aaatgactaa ttaagttagt caaggcgcca
      181 tcctcatgaa aactgtgtaa cataataacc gaagtgtcga aaaggtggca ccttgtccaa
      241 ttgaacacgc tcgatgaaaa aaataagata tatataaggt taagtaaagc gtctgttaga
      301 aaggaagttt ttcctttttc ttgctctctt gtcttttcat ctactatttc cttcgtgtaa
      361 tacagggtcg tcagatacat agatacaatt ctattacccc catccatact ctagaactag
      421 tggatccccc atcacaagtt tgtacaaaaa agcaggcttc acaaaatgtt tttcaacaga
      481 ctaagcgctg gcaagctgct ggtaccactc tccgtggtcc tgtacgccct tttcgtggta
      541 atattacctt tacagaattc tttccactcc tccaatgttt tagttagagg tgtggagccc
      601 atcagcctgg gactggccct ggccggcgtc ctcaccggct acatctaccc gcgtctctac
      661 tgcctcttcg ccgagtgctg cgggcagaag cggagcctta gccgggaggc actgcagaag
      721 gatctggacg acaacctctt tggacagcat cttgcaaaga aaatcatctt aaatgccgtg
      781 tttggtttca taaacaaccc aaagcccaag aaacctctca cgctctccct gcacgggtgg
      841 acaggcaccg gcaaaaattt cgtcagcaag atcatcgcaa
//
