LOCUS       sequence-374765-        9058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of TAOK2 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9058)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-TAOK2-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..9058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     352..375
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    424..1012
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             530..541
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     939..959
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    1041..1061
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             complement(1067..1837)
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             complement(1844..1885)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    1887..1911
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5071..5095)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(5096..6307)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(5240..5259)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(6307..6327)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    6359..6475
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6742..6786)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6971..7204
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7697..7822
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7869..8049
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8050..8276)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(8304..8324)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8304..8322)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8343..8359)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8343..8359)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8356..8378)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8367..8383
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8391..8397,8398..8415,8416..8421))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8436..8457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8574..8591)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8825..8844)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcssccgccc ncscccckgr gcccgcccct gagcccgccc ccggacccac cccttcccag
       61 cctctgagcc cagaaagcga aggagcaaag ctgctattgg ccgctgcccc aaaggcctac
      121 ccgcttccat tgctcagcgg tgctgtccat ctgcacgaga ctagtgagac gtgctacttc
      181 catttgtcac gtcctgcacg acgcgagctg cggggcgggg gggaacttcc tgactagggg
      241 aggagtagaa ggtggcgcga aggggccacc aaagaacgga gccggttggc gcctaccggt
      301 ggatgtggaa tgtgtgcgag gccagaggcc acttgtgtag cgccaagtgc ccagcggggc
      361 tgctaaagcg catgctccag actgccttgg gaaaagcgcc tcccctaccc ggtagaattc
      421 caggcgggga ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg
      481 gaagaggccg cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg
      541 aagggacgta gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc
      601 atggaaagga cgtcagcttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag
      661 cccctgtcca gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg
      721 ggaaagcgaa agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg
      781 ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct
      841 cataaagaga cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag
      901 caatagcatg atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat
      961 agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga ttgtcgagcg
     1021 gccgccactg tgctggatat caactatgta taataaagtt ggcttacttt tcggcagacc
     1081 gcagactgat ttctccgccc atccagggtt tgataactcc gtcctgttcc agcagcaggg
     1141 ctccctgttt atcaatcccc cggctaatcc cgaaaatctc tttgtcccca atgatcagct
     1201 tcacgggtct attgatgaag ttatccagct tctcccaccg tgacagatat ggagccaggc
     1261 cttcctgctc gaacagttcc agggctgccc gcagctctcg gatcagcata gcggccagag
     1321 tattcctgtc caggttaatc cctgcttcct gcagtgtgat ccagccctga ttgaccacgc
     1381 tttcctccac gcgcctcata gccacgttaa tcccggcgcc aatgacgatc tgtgcagcat
     1441 cgcctgttat tccggccagc tccaccagga tgcctgccag ctttctatcc tgcagataca
     1501 ggtcattggg ccatttgact cgcaccttgt ctgctcccag ctttcgcagc gcttctgcca
     1561 tgacaattcc gatgaccggg cccaggccga ttgctgctgg tccccgcttc aggcgccaaa
     1621 acatactcag gtacaggtta gctccaaaag gagagaacca tttcctccct ctgcttcctc
     1681 tccctgcctg ctgatattct gcaatgcaag catccccact cttcagctcg ccgattcgat
     1741 ccagcaggta ctgattggtg gagtcgacca caggcaggac tgccacgctc ccgccgtcca
     1801 gctgtcccag aatctgttta gcgttcagca gcgggatgct agcggtgctg tccaggccca
     1861 gcagggggtt ggggatgggc ttgccgccca ctttgtacaa gaaagctggg tttgaggaat
     1921 agaagtggga agaaccattg aggatgtggg aagcgacact ggtgctgcgg ctcaggggcc
     1981 cgggcaccgc ggcagcaggg gggcccacat tctcactgcc actgcccccc gaggctgccc
     2041 gccgcagggg ctgggggctg tttcttagca gcagcagggc tccgccacgg ggtgtcccac
     2101 tttgtgtggg gggccccagc cagtttgggg ggcctggggg agccagcaga gacggctgac
     2161 gccaggctgg agggggcatg cctggtggag gagggccatg gctccagtgt gccccagaac
     2221 ggggaacggg acgggagggc caggctgggg cagggggtgg agcaggatag ccctgggcag
     2281 cagcttcagc cgggatgccc cccagagcca tgctggagaa gcccagcctc atgctttccg
     2341 catcgaaggc ctcgatctca cgggcctgcc gctcaagcag actgcggatt cgctcggagc
     2401 gtcctgtctg cagggccagc agctcctctt ccacccgctg ctccagcagt gcccgccgca
     2461 gcgcgaccct ctgctccagc tcccgcagct ccctctcgtg ctggctctct gtgcggatct
     2521 tgatcttgct ctggtaagcg ttgagcagct ccagctcctg ttgaagctgc tgccgaaggg
     2581 cctggaactc tgcctcctgg gtctcatcaa gccgcagcgc ctgtgagctg agcatctctg
     2641 agatggactg gtcatactgc tccgccaaga tcgccagctt gcgggtctgc tcttccttga
     2701 gccgcttaag gaggctcttg tgctgagctt tgggcgtggt ctccagcaag tgtgctcgca
     2761 gagccttgta ctgccgagtc tggatcttac acgtctcctg gaactgcttc ttgatctgca
     2821 gctccttaga tttgaggctc ttgggctgct ggcgaacctg ggccgcatgc ttctgccgca
     2881 actcttgctc acgccgcttg ttgtactcca gctggttgcc cagctccgtc tggtgctgca
     2941 ggcgggtgag ctcagcccgc gtgcgctgca cggcctggag ctgccgcagc tccagctccc
     3001 gcgtggcctc gtgctgccga agcagcagtg cacactccaa gtccttctgg gtctgcttct
     3061 tgttcaggtc ctcccgcagc aggtcctggt ccaggctgtg ccgagccagc aacatcttgc
     3121 gcttgtactg gcgacactgc agctcaaagt actggcgctg ccgccgcagc agccctgctt
     3181 cctcctccgc ctggcactgc tggagctgct ccttctgccg cagcagccac tcggccttct
     3241 cccgcttggg agtgctgggg ttctcctgga gctcctcctt cagctgttcc ttgcgaagtt
     3301 tgtaggtccg cttctgtgcc tccagcaggg cagccagctc cttcttctgc tgcccaagga
     3361 tgtgctgctg gaacttccgc tcctcggcct gggcagctcg tgcctccttc tcacctatgg
     3421 cctggtgccg ccgggccagc ttttctgcct ctgccccaaa gccagcccgc tgcgcctcaa
     3481 gctcccgctg cagccgtgca ctgtgctcct cccgttcacc cctcagccgt gactccaggg
     3541 ccagcagctg cttctggtgc tgtcgtcgca tccgcttata gccgctcagc tgctcccgca
     3601 gcgcagagtc ctgctcatgc tcctggatct gacggctgac cagggaggcg gttcggatgg
     3661 tggcaaagtg gtctcggtta cggcagtagg cccggcggcg ggcagaagag gtggtggaag
     3721 tgggagctgg ggctgcaggc ggctggagag ggctgggggt tatctctggc tggtaggggt
     3781 catcatatag gttgtcagag cccggcagcc ggtggataat ggagctgtga gaggtgactg
     3841 tgtgctcccc ctcctgcatc atggccatct cccgggcttc agggccttct tcctcctcct
     3901 cctcttcctc ctcctcctcc tcctcttcct cgttgtctga ggcatctgct aggctgttga
     3961 cggagctgct ctggctggag gcgctgatgg acatgctggg cactgagtgg ctactctcga
     4021 ggctggtcag agtcccggcc cggtgcatgt agggctcggc ctcctcttcc tcctctgggg
     4081 cctcggcacc agggccgttg ggtgcctctt ggaacaggat cttcttcatc ttgcggtact
     4141 gcaggttgtc cagctcccgc acggcatcct tggtcctctg gatcaggtcc atgatgactg
     4201 tgggtggccg ctcccggagc acaaagcggt gcttcaggag aacctctgag gttggtctgt
     4261 cttgagggat tttctgaaga caggagtcga caaaattccg gaagtactca gaccagtgtc
     4321 ctgactggag cacgggggat tcgttctgtg caatgtggta taaggcactc atcgcattca
     4381 tgttaaagag cggtggtttc cgttcagcca gctcgatgca ggttatcccc aaggaccaga
     4441 cgtccacttt gccatcgtac tgcccctcat ccatggccag gatcacctcg ggtgccatcc
     4501 agtatggggt gcccacgaag gagttggcag gtgccatgat ggacgcagaa ccaaagtccc
     4561 ctagcttcac taaccctggc tctgacagca ggatgtttcc agccttcaca tccctatgga
     4621 tcatgttgtg ggagtgcaga tatgccaggc cctgaagcgc cccgtgggtc acagctgcga
     4681 tctctacctc ctgaaggggt ttcttgtgca cttctagaag gtcagaagct gagcccaggc
     4741 aatactccat taccagccaa gccgtgtgct ccctcaggta acagccccgg tactgaatgg
     4801 tgttgggatg ccggagcttc tgtaagaacc gcacctcctt gatgatgtct tgccatttct
     4861 cattggactg cttcccactg taggacatct tcttgatggc caccacctca ctattccgga
     4921 catcccgggc aaagtatacg gctccaaagc tgccatggcc aatttcccgg aggtcagaga
     4981 agagcttttc tgggtcatcc ttgaagaaga gctcagccac atctgggtcc ttcaggctcc
     5041 cggcccggcc cccagctggc atggtggcta agcctgcttt tttgtacaaa cttgtgtcta
     5101 acaaaaaagc caaaaacggc cagaatttag cggacaattt actagtctaa cactgaaaat
     5161 tacatattga cccaaatgat tacatttcaa aaggtgccta aaaaacttca caaaacacac
     5221 tcgccaaccc cgagcgcata gttcaaaacc ggagcttcag ctacttaaga agataggtac
     5281 ataaaaccga ccaaagaaac tgacgcctca cttatccctc ccctcaccag aggtccggcg
     5341 cctgtcgatt caggagagcc taccctaggc ccgaaccctg cgtcctgcga cggagaaaag
     5401 cctaccgcac acctaccggc aggtggcccc accctgcatt ataagccaac agaacgggtg
     5461 acgtcacgac acgacgaggg cgcgcgctcc caaaggtacg ggtgcactgc ccaacggcac
     5521 cgccataact gccgcccccg caacagacga caaaccgagt tctccagtca gtgacaaact
     5581 tcacgtcagg gtccccagat ggtgccccag cccatctcac ccgaataaga gctttcccgc
     5641 attagcgaag gcctcaagac cttgggttct tgccgcccac catgcccccc accttgtttc
     5701 aacgacctca cagcccgcct cacaagcgtc ttccattcaa gactcgggaa cagccgccat
     5761 tttgctgcgc tccccccaac ccccagttca gggcaacctt gctcgcggac ccagactaca
     5821 gcccttggcg gtctctccac acgcttccgt cccaccgagc ggcccggcgg ccacgaaagc
     5881 cccggccagc ccagcagccc gctactcacc aagtgacgat cacagcgatc cacaaacaag
     5941 aaccgcgacc caaatcccgg ctgcgacgga actagctgtg ccacacccgg cgcgtcctta
     6001 tataatcatc ggcgttcacc gccccacgga gatccctccg cagaatcgcc gagaagggac
     6061 tacttttcct cgcctgttcc gctctctgga aagaaaacca gtgccctaga gtcacccaag
     6121 tcccgtccta aaatgtcctt ctgctgatac tggggttcta aggccgagtc ttatgagcag
     6181 cgggccgctg tcctgagcgt ccgggcggaa ggatcaggac gctcgctgcg cccttcgtct
     6241 gacgtggcag cgctcgccgt gaggaggggg gcgcccgcgg gaggcgccaa aacccggcgc
     6301 ggaggccaac ttttctatac aaagttgata tctgcagaat tccaccacac tggacgataa
     6361 aattttgaat tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
     6421 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaagttaa
     6481 ccgataccgt cgagatccgt tcactaatcg aatggatctg tctctgtctc tctctccacc
     6541 ttcttcttct attccttcgg gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa
     6601 cgataatggt gaatatccct gcctaactct attcactata gaaagtacag caaaaactat
     6661 tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt
     6721 tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc
     6781 attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt
     6841 gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt
     6901 attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc
     6961 ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg
     7021 gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct
     7081 cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag
     7141 cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc
     7201 tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc
     7261 tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc
     7321 cctcatatct cctcctccag gtctgaagat cagcggccgc ttgctgtgcg gtggtcttac
     7381 ttttgttttg ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc
     7441 tttgatgcac acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc
     7501 ctgtctgaag ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta
     7561 acaggccagg attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta
     7621 atttatattt tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc
     7681 tcccccgctt aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca
     7741 aaatttttgg cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa
     7801 gccgagtcct gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg
     7861 ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
     7921 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
     7981 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
     8041 gagagacccg tttatgtatc gagctaggca cttaaataca atatctctgc aatgcggcaa
     8101 ttcagtggtt cgtccaatcc atgtcagacc cgtctgttgc cttcctaata aggcacgatc
     8161 gtaccacctt acttccacca atcggcatgc acggtgcttt ttctctcctt gtaaggcatg
     8221 ttgctaactc atcgttacca tgttgcaaga ctacaagagt attgcataag actacattaa
     8281 gcttgcagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     8341 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8401 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8461 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8521 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8581 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8641 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8701 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8761 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8821 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8881 ctgccgctta ccggatacct gtccrccttt ctcccttcgg gaagcgtggc gctttctcat
     8941 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9001 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagt
//
