LOCUS       sequence-374768-        9553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1
            constant region with CS1 capture sequence in stem loop, and UCOE 
            EF1alpha driving PURO-BFP marker expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9553)
  AUTHORS   Replogle JM, Bonnar JL, Pogson AN, Liem CR, Maier NK, Ding Y, 
            Russell BJ, Wang X, Leng K, Guna A, Norman TM, Pak RA, Ramos DM, 
            Ward ME, Gilbert LA, Kampmann M, Weissman JS, Jost M
  TITLE     Maximizing CRISPRi efficacy and accessibility with dual-sgRNA 
            libraries and optimal effectors.
  JOURNAL   eLife. 2022 Dec 28;11:e81856. doi: 10.7554/eLife.81856.
  PUBMED    36576240
REFERENCE   2  (bases 1 to 9553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2022-12-28"; volume: "11:e81856. doi"; pages: " 
            10.7554/eLife.81856"
COMMENT     Sequence Label: pJR100
FEATURES             Location/Qualifiers
     source          1..9553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..82)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        254..633
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        635..833
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     788..808
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             851..1031
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1078..1203
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1700..1933
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2118..2162
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2460..2577
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2830..2863)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2867..2887
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        3789..4967
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4020..4958
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4915..4935
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4979..5575
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4979..4998)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5475..5495
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5585..5638
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5651..6349
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     protein_bind    complement(6369..6402)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6458..7046
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6511..6531)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6929..6940)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7049..7065)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7050..7066)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7575..7755
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7817..8405)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7897..7916)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8576..9367,9368..9436))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9199..9218
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9437..9541)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
       61 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
      121 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
      181 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
      241 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
      301 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      361 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      421 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      481 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      541 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      601 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      661 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      721 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      781 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt
      841 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      901 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
      961 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     1021 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     1081 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     1141 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     1201 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     1261 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     1321 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     1381 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     1441 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     1501 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca
     1561 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     1621 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     1681 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     1741 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     1801 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     1861 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     1921 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     1981 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     2041 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     2101 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     2161 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     2221 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     2281 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     2341 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     2401 tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat
     2461 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     2521 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     2581 gtttattaca gggacagcag agatccagtt tggttagtac cgggcccgct ctagagatcc
     2641 gacgcgccat ctctaggccc gcgccggccc cctcgcacgg acttgtggga gaagctcggc
     2701 tactcccctg ccccggttaa tttgcatata atatttccta gtaactatag aggcttaatg
     2761 tgcgataaaa gacagataat ctgttctttt taatactagc tacattttac atgataggct
     2821 tggatttcta taacttcgta tagcatacat tatacgaagt tataaacagc acaaaaggaa
     2881 actcacccta actgtaaagt aattgtgtgt tttgagacta taagtatccc ttggagaacc
     2941 accttgttgg accaggatgg gcaccacccg tttaagagct aagctggaaa cagcatagca
     3001 agtttaaata aggctagtcc gttatcaact tggccgcttt aaggccggtc ctagcaaggc
     3061 caagtggcac cgagtcggtg ctttttttct cgagtactag gatccattag gcggccgccg
     3121 ggaggtggtc cctgcagtta cgccaatgat aacccccgcc agaaaaatct tagtagcctt
     3181 ccctttttgt tttccgtgcc ccaactcggc ggattgactc ggccccttcc ggaaacaccc
     3241 gaatcaactt ctagtcaaat tattgttcac gccgcaatga cccacccctg gcccgcgtct
     3301 gtggaactga cccctggtgt acaggagagt tcgctgctga aagtggtccc aaaggggtac
     3361 tagtttttaa gctcccaact ccccctcccc cagcgtctgg aggattccac accctcgcac
     3421 cgcaggggcg aggaagtggg cggagtccgg ttttggcgcc agccgctgag gctgccaagc
     3481 agaaaagcca ccgctgagga gactccggtc actgtcctcg ccccgcctcc cccttccctc
     3541 cccttgggga ccaccgggcg ccacgccgcg aacggtaagt gccgcggtcg tcggcgcctc
     3601 cgccctcccc ctagggcccc aattcccagc gggcgcggcg cgcggcccct ccccccgccg
     3661 ggcgcgcgcc cgctgccccg cccttcgtgg ccgcccggcg tgggcggtgc cacccctccc
     3721 cccggcggcc ccgcgcgcag ctcccggctc cctccccctt cggatgtggc ttgagctgta
     3781 ggcgcggaga tgcatgtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     3841 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     3901 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     3961 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     4021 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
     4081 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
     4141 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
     4201 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
     4261 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
     4321 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
     4381 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
     4441 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
     4501 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
     4561 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
     4621 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     4681 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     4741 acctcgatta gttctcgcgc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     4801 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     4861 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     4921 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagct agcccaccat
     4981 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     5041 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     5101 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     5161 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     5221 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     5281 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     5341 cgcgtggttc ctggccaccg tcggcgtgtc gcccgaccac cagggcaagg gtctgggcag
     5401 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     5461 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     5521 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccggatc
     5581 gggagagggc agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccacc
     5641 ggtcgccacc agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac
     5701 cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
     5761 ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
     5821 ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga
     5881 cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga
     5941 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
     6001 cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact
     6061 cggctgggag gccttcaccg agaccctgta ccccgctgac ggcggcctgg aaggcagaaa
     6121 cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacatca agaccacata
     6181 tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag
     6241 actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt
     6301 ggccagatac tgcgacctcc ctagcaaact ggggcacaag cttaattaag aattcgtcga
     6361 gggacctaat aacttcgtat agcatacatt atacgaagtt atacatgttt aagggttccg
     6421 gttccactag gtacaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     6481 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     6541 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     6601 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     6661 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     6721 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     6781 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6841 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     6901 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6961 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     7021 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgatcga gacctagaaa
     7081 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     7141 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     7201 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     7261 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     7321 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
     7381 ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag
     7441 gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     7501 aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc
     7561 atccggactg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7621 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7681 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7741 ggaaaatctc tagcagcatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7801 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7861 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7921 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7981 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8041 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8101 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8161 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8221 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8281 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8341 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8401 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8461 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8521 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8581 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8641 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8701 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8761 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8821 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8881 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8941 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9001 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9061 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9121 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9181 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9241 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9301 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9361 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9421 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9481 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9541 gcacatttcc ccg
//
