LOCUS       sequence-374770-        9790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yTRAP sensor of [PSI+], detects aggregation of Sup35.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9790)
  AUTHORS   Newby GA, Kiriakov S, Hallacli E, Kayatekin C, Tsvetkov P, Mancuso 
            CP, Bonner JM, Hesse WR, Chakrabortee S, Manogaran AL, Liebman SW, 
            Lindquist S, Khalil AS
  TITLE     A Genetic Tool to Track Protein Aggregates and Control Prion 
            Inheritance.
  JOURNAL   Cell. 2017 Nov 2;171(4):966-979.e18. doi: 
            10.1016/j.cell.2017.09.041. Epub 2017 Oct 19.
  PUBMED    29056345
REFERENCE   2  (bases 1 to 9790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.041"; journalName: "Cell"; date: "2017-11-2-
            2"; volume: "171"; issue: "4"; pages: "966-979.e18"
COMMENT     Sequence Label: pGAN200
FEATURES             Location/Qualifiers
     source          1..9790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1510..1534
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2295..2319)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2357..2377
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2981..3070
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             3077..3166
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     regulatory      4303..4312
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      4779..4966
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4834..4858)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            5041..6172
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     CDS             5391..5963
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      5975..6172
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     6735..6751
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6735..6751
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6765..6784)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6766..6784)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6791..6808)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6791..6807)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6800..6822)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7107..7126)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7239..7260
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7414..7433)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7533..7555
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7593..7611)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7679..7783
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7784..7852,7853..8644)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8002..8021)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8815..9403
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9304..9323
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9557..9574
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9691..9712
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    9767..9783
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(9772..9790,1..4)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca ctagtccgag gcctcgagat ccgatatcgc
       61 cgtggcggcc gcaattatcc tgggcacgag tgaaacaaag ctaaaacctt tatttagcat
      121 ggccattgaa tgtaacaatt atatatatcg caagcacaaa aaatcaagga gagagaacta
      181 ccactttgtt catgtgtaca atgttcatta tctccataag caaaaaaaaa aaaatagaaa
      241 acatatgcta taaggttgat attctcacga gtaagcggca cttgctactt attgacattg
      301 cagatttttg gctacagaaa tagtatatta gagattataa ttgctaatca aatcaaaata
      361 taaaattagt aaaccaaacc atttataccc ttccttagta gttatggatt gttttttaat
      421 gatatttctg caaaccaaag aaagattgtt atccagatag aatttagttt tgatattcat
      481 ttttttgttg aagattgaac gccatatctg ggcctcataa ttcaaaagac ggtgccatta
      541 tcggtagcgt ttcgcattgt actggatttc agaaatttca cagttgatga atcgaaaaga
      601 atggtctcat tgcaacacgt aaggttaaga tgtccctttt taccattata ggcaataaat
      661 gaatcataaa acgaccgtat actggtgaaa tagtagggag aacgagtacc tgtagtaaaa
      721 agtataaatc atagttaatc gggcaatgtc cctcgatcaa ggagtattgt gtcatgttcg
      781 agacaaacgc caacattttt gtttcttttg gacaaatgtt gtttgcattt atgatccgtt
      841 atattttgat ctaatgtaga gttgcacgta gttcttactg gcaaagaaat cgatgcatac
      901 caaaaaagaa taaaggtgat atttgatctt taccgtttag ttccaacgta aaattgtgcc
      961 tttggactta aaatggcgtc gtacgctgca ccgagtgcaa ccacacaaaa atcatacaac
     1021 gaatggtata tgcttcattt ctttgtttcg cattagctgc gctatttgac tcaaattatt
     1081 attttttact aagacgacgc gtcacagtgt tcgagtctgt gtcatttctt ttgtaattct
     1141 cttaaaccac ttcataaagt tgtgaagttc atagcaaaat tcttccgcaa aaagatgaat
     1201 cttagttctc agcccaccaa aagaggtaca tgctaagatc atacagaagt tattgtcact
     1261 tcttaccttg ctcttaaatg tacattacaa ccgggtatta tatcttacat catcgtataa
     1321 tatgatcttt ctttatggag aaaatttttt tttcactcga ccaaagctcc cattgcttct
     1381 gaagagtgta gtgtatattg gtacatcttc tcttgaaaga ctccattgta ctgtaacaaa
     1441 aagcggtttc ttcatcgact tgctcggaat aacatctata tctgcccact agcaacagct
     1501 agccccatca caagtttgta caaaaaagca ggctatgtcg gattcaaacc aaggcaacaa
     1561 tcagcaaaac taccagcaat acagccagaa cggtaaccaa caacaaggta acaacagata
     1621 ccaaggttat caagcttaca atgctcaagc ccaacctgca ggtgggtact accaaaatta
     1681 ccaaggttat tctgggtacc aacaaggtgg ctatcaacag tacaatcccg acgccggtta
     1741 ccagcaacag tataatcctc aaggaggcta tcaacagtac aatcctcaag gcggttatca
     1801 gcagcaattc aatccacaag gtggccgtgg aaattacaaa aacttcaact acaataacaa
     1861 tttgcaagga tatcaagctg gtttccaacc acagtctcaa ggtatgtctt tgaacgactt
     1921 tcaaaagcaa caaaagcagg ccgctcccaa accaaagaag actttgaagc ttgtctccag
     1981 ttccggtatc aagttggcca atgctaccaa gaaggttggc acaaaacctg ccgaatctga
     2041 taagaaagag gaagagaagt ctgctgaaac caaagaacca actaaagagc caacaaaggt
     2101 cgaagaacca gttaaaaagg aggagaaacc agtccagact gaagaaaaga cggaggaaaa
     2161 atcggaactt ccaaaggtag aagaccttaa aatctctgaa tcaacacata ataccaacaa
     2221 tgccaatgtt accagtgctg atgccttgat caaggaacag gaagaagaag tggatgacga
     2281 agttgttaac gatgacccgg ctttcttgta caaagtggtg atgggctgca ggaattcgat
     2341 atcaagctta atggctccaa agaagaagag aaaggttggt attcatggtg ttccagttga
     2401 tggtggttta caagctccac caactgatgt ttctttgggt gatgaattgc atttggatgg
     2461 tgaagatgtt gctatggctc atgctgatgc tttggatgat tttgatttgg atatgttggg
     2521 tgacggtgat tctccaggtc caggttttac tccacatgat tctgctccat atggtgcttt
     2581 agatatggct gactttgaat tcgaacaaat gttcaccgat gccttgggta ttgatgaata
     2641 tggtggtggt gctagaccag gtgaaagacc atttcaatgt agaatctgca tgagaaactt
     2701 ctccagacaa gatagattgg atagacatac cagaactcat actggtgaaa agccattcca
     2761 atgcagaata tgtatgagaa atttttccca aaaagaacac ttggccggtc atttgagaac
     2821 acacacaggt gaaaaacctt ttcaatgcag aatatgcatg agaaacttta gtagaagaga
     2881 caacttgaac agacacttga aaacccattt gagaggttct gccggcgaat tggctttgaa
     2941 attggctggt ttggatatca attctggttc tgctgctaga tacccatatg atgttccaga
     3001 ttatgctggt tacccttacg atgttcctga ctacgcaggt tcttatccat acgatgtacc
     3061 tgattatgcc tcaagatatc cttatgacgt ccctgattac gccggttatc catatgacgt
     3121 accagactac gctggtagtt acccatacga cgtcccagat tacgcttctt cataatgagg
     3181 gccctcatgt aatttcttgc aaacataagt aaatgcaaac acaataatac cgatcataaa
     3241 gcattttctt ctatattaaa aaacaaggtt taataaagct gttatatata tatatatata
     3301 tatagacgta taattagttt agttcttttt gtaccatata ccataaacaa ggtaaacttc
     3361 acctctcaat atatctagaa tttcataaaa atatctagca aggtttcaac tccttcaatc
     3421 acgttttcat cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     3481 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     3541 aaaatattaa cgtttacaat ttcctgatgc ggtattttct ttaattaaag agtgaggact
     3601 cgaaaatatt aatagagtga ggactcgaaa atattaatag agtgaggact cgaaaatatt
     3661 aatagagtga ggactcgaaa atattaatat cgatagagtg aggactcgaa aatattaata
     3721 gagtgaggac tcgaaaatat taatgaattc agagtgagga ctcgaaaata ttaatagagt
     3781 gaggactcga aaatattaat ggccggccca gatccgccag gcgtgtatat atagcgtgga
     3841 tggccaggca actttagtgc tgacacatac aggcatatat atatgtgtgc gacgacacat
     3901 gatcatatgg catgcatgtg ctctgtatgt atataaaact cttgttttct tcttttctct
     3961 aaatattctt tccttataca ttaggacctt tgcagcataa attactatac ttctatagac
     4021 acacaaacac aaatacacac actgtttaaa caaaaaatgg gcgaggagga taacatggcc
     4081 tctctcccag cgacacatga gttacacatc tttggctcca tcaacggtgt ggactttgac
     4141 atggtgggtc agggcaccgg caatccaaat gatggttatg aggagttaaa cctgaagtcc
     4201 accaagggtg acctccagtt ctccccctgg attctggtcc ctcatatcgg gtatggcttc
     4261 catcagtacc tgccctaccc tgacgggatg tcgcctttcc aggccgccat ggtagatggc
     4321 tccggatacc aagtccatcg cacaatgcag tttgaagatg gtgcctccct tactgttaac
     4381 taccgctaca cctacgaggg aagccacatc aaaggagagg cccaggtgaa ggggactggt
     4441 ttccctgctg acggtcctgt gatgaccaac tcgctgaccg ctgcggactg gtgcaggtcg
     4501 aagaagactt accccaacga caaaaccatc atcagtacct ttaagtggag ttacaccact
     4561 ggaaatggca agcgctaccg gagcactgcg cggaccacct acacctttgc caagccaatg
     4621 gcggctaact atctgaagaa ccagccgatg tacgtgttcc gtaagacgga gctcaagcac
     4681 tccaagaccg agctcaactt caaggagtgg caaaaggcct ttaccgatgt gatgggcatg
     4741 gacgagctgt acaagtaagg cgcgccactt ctaaataagc gaatttctta tgatttatga
     4801 tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt
     4861 aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt
     4921 ctcaggtata gtatgaggtc gctcttattg accacacctc taccggcaga tccgctaggg
     4981 ataacagggt aatatagatc tgtttagctt gcctcgtccc cgccgggtca cccggccagc
     5041 gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga
     5101 tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata
     5161 cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag
     5221 cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga
     5281 aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc
     5341 ttgctaggat acagttctca catcacatcc gaacatcaat tgaaacaacc atgggtacca
     5401 ctcttgacga cacggcttac cggtaccgca ccagtgtccc gggggacgcc gaggccatcg
     5461 aggcactgga tgggtccttc accaccgaca ccgtcttccg cgtcaccgcc accggggacg
     5521 gcttcaccct gcgggaggtg ccggtggacc cgcccctgac caaggtgttc cccgacgacg
     5581 aatcggacga cgaatcggac gacggggagg acggcgaccc ggactcccgg acgttcgtcg
     5641 cgtacgggga cgacggcgac ctggcgggct tcgtggtcgt ctcgtactcc ggctggaacc
     5701 gccggctgac cgtcgaggac atcgaggtcg ccccggagca ccgggggcac ggggtcgggc
     5761 gcgcgttgat ggggctcgcg acggagttcg cccgcgagcg gggcgccggg cacctctggc
     5821 tggaggtcac caacgtcaac gcaccggcga tccacgcgta ccggcggatg gggttcaccc
     5881 tctgcggcct ggacaccgcc ctgtacgacg gcaccgcctc ggacggcgag caggcgctct
     5941 acatgagcat gccctgcccc taaagatctt cagtactgac aataaaaaga ttcttgtttt
     6001 caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt
     6061 tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc
     6121 gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat
     6181 tcgatactaa cgccgccatc cagtgtcgaa aacgagctcg aattcctggg ggaacaactt
     6241 cacagaatgt tttgtcatat tgtcgaagtg gtcacaaaac aagagaagtt ccgccaatta
     6301 taaaaaggga acccgtatat ttcagcttca cggatgattt ccagggtgag agtactgtat
     6361 atgggcttac gatagaaggc cataaaaatt tcttgcttgg caacaaaata gaagtgaaat
     6421 catgtcgagg ctgctgtgtg ggagaacagc ataaaatatc acaaaaaaag aatctaaaac
     6481 actgtgttgc ttgtcccaga aagggaatca agtattttta taaagattgg agtggtaaaa
     6541 atcgagtatg tgctagatgc tatggaagat acaaattcag cggtcatcac tgtataaatt
     6601 gcaagtatgt accagaagca cgtgaagtga aaaaggcaaa agacaaaggc gaaaaattgg
     6661 gcattacgcc cgaaggtttg ccagttaaag gaccagagtg tataaaatgt ggcggaatct
     6721 tacagtgcgg ccgctctaga actagtggat cgatccccaa ttcgccctat agtgagtcgt
     6781 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     6841 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     6901 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
     6961 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     7021 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     7081 tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     7141 gttcgccggc tttccccgtc aagctctaaa tcggggtggg ccatcgccct gatagacggt
     7201 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     7261 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
     7321 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     7381 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7441 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     7501 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     7561 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     7621 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     7681 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7741 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7801 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7861 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7921 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7981 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     8041 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     8101 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     8161 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     8221 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8281 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     8341 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8401 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8461 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8521 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8581 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8641 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8701 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8761 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8821 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8881 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8941 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     9001 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9061 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9121 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9181 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9241 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9301 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9361 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9421 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     9481 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     9541 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     9601 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     9661 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     9721 ccaggcttta cactttatgc ttccgcggct cgtatgttgt gtggaattgt gagcggataa
     9781 caatttcaca
//
