LOCUS       sequence-374778-         493 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for Gal4-induced Cre recombination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Yoshihara M, Nishino T, Sambe N, Nayakama T, Radtke F, Mizuno S, 
            Takahashi S
  TITLE     Generation of a Gal4-dependent gene recombination and illuminating 
            mouse.
  JOURNAL   Exp Anim. 2022 Aug 5;71(3):385-390. doi: 10.1538/expanim.21-0202. 
            Epub 2022 Apr 21.
  PUBMED    35444103
REFERENCE   2  (bases 1 to 493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1538/expanim.21-0202"; journalName: "Exp Anim"; date: 
            "2022-08-5- 5"; volume: "71"; issue: "3"; pages: "385-390"
COMMENT     Sequence Label: UAS-Cre-T2A-miRFP670
FEATURES             Location/Qualifiers
     source          1..493
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..164
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        183..421
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     368..389
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     regulatory      441..450
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tacctgagct cgctagcctc gaggatatca agatctggcc tcggcggcca agcttgcatg
       61 cctgcaggtc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
      121 tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggaga ctctagcgag
      181 cgccggagta taaatagagg cgcttcgtct acggagcgac aattcaattc aaacaagcaa
      241 agtgaacacg tcgctaagcg aaagctaagc aaataaacaa gcgcagctga acaagctaaa
      301 caatctgcag taaagtgcaa gttaaagtga atcaattaaa agtaaccagc aaccaagtaa
      361 atcaactgca actactgaaa tctgccaaga agtaattatt gaatacaaga agagaactct
      421 gaatagggaa ttgggaattc gccaccatgg ccaatttact gaccgtacac caaaatttgc
      481 ctgcattacc ggt
//
