LOCUS       sequence-37478-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Subach FV, Subach OM, Gundorov IS, Morozova KS, Piatkevich KD, 
            Cuervo AM, Verkhusha VV
  TITLE     Monomeric fluorescent timers that change color from blue to red 
            report on cellular trafficking.
  JOURNAL   Nat Chem Biol. 2009 Feb;5(2):118-26. Epub 2009 Jan 11.
  PUBMED    19136976
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol."; date: "2009-02"; volume: "5(2)"; pages: "118-26. Epub 2009 
            Jan 11"
COMMENT     Sequence Label: pSlow-FT-N1
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..98
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..816
                     /codon_start=1
                     /product="blue-to-red fluorescent timer derivative of 
                     mCherry (Subach et al., 2009)"
                     /label=Slow-FT
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHVEGSVNGHEFEIVGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSRAYVKHPADIPDYWKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDVEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(251..269)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     519..538
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt
       61 ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt gagcaagggc
      121 gaggaggata acatggccat catcaaggag ttcatgcgtt tcaaggtgca cgtggagggc
      181 tccgtgaacg gccacgagtt cgagatcgtg ggcgagggcg agggccgccc ctacgagggc
      241 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      301 ctgtcccctc agttcatgta cggctccagg gcctacgtga agcaccccgc cgacatcccc
      361 gactactgga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      421 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      481 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      541 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
      601 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgttgaggt caagaccacc
      661 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
      721 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
      781 cactccaccg gcggcatgga cgagctgtac aagtaagcgg ccgcgactct agatcataat
      841 cagccatacc acatttgta
//
