LOCUS       sequence-37482-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Morozova KS, Piatkevich KD, Gould TJ, Zhang J, Bewersdorf J, 
            Verkhusha VV
  TITLE     Far-red fluorescent protein excitable with red lasers for flow 
            cytometry and superresolution STED nanoscopy.
  JOURNAL   Biophys J. 2010 Jul 21;99(2):L13-5.
  PUBMED    20643047
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biophys 
            J."; date: "2010-07-21"; volume: "99(2)"; pages: "L13-5"
COMMENT     Sequence Label: pTagRFP657-N1
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    33..113
                     /label=MCS
                     /note="multiple cloning site"
     CDS             121..822
                     /codon_start=1
                     /product="far-red monomeric fluorescent protein with an 
                     emission peak at 657 nm (Morozova et al., 2010)"
                     /label=TagRFP657
                     /note="derived from mKate"
                     /translation="MSELITENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTQRIKV
                     VEGGPLPFAFDILATSFMYGSHTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEAHTEMLYPADGGLEGRTALALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDYRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 tagcagagct ggtttagtga accgtcagat ccgctagcgc taccggactc agatctcgag
       61 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc
      121 atgagcgagc tgatcaccga gaacatgcac atgaagctgt acatggaggg caccgtgaac
      181 aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
      241 cagagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc
      301 agcttcatgt acggcagtca caccttcatc aaccacaccc agggcatccc cgacttctgg
      361 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
      421 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
      481 atcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg
      541 gaggcccaca ccgagatgct gtaccccgct gacggcggcc tggaaggcag aaccgcgctg
      601 gccctgaagc tcgtgggcgg gggccacctg atctgcaact tcaagaccac atacagatcc
      661 aagaaacccg ctaagaacct caagatgccc ggcgtctact atgtggacta cagactggaa
      721 agaatcaagg aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga
      781 tactgcgacc tccctagcaa actggggcac aagcttaatt aaagcggccg cgactctaga
      841 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
      901 tccccctgaa cctgaaacat aaaatgaatg c
//
