LOCUS       sequence-374822-        9527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant 
            region, and UCOE EF1alpha driving PURO-BFP marker expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Replogle JM, Bonnar JL, Pogson AN, Liem CR, Maier NK, Ding Y, 
            Russell BJ, Wang X, Leng K, Guna A, Norman TM, Pak RA, Ramos DM, 
            Ward ME, Gilbert LA, Kampmann M, Weissman JS, Jost M
  TITLE     Maximizing CRISPRi efficacy and accessibility with dual-sgRNA 
            libraries and optimal effectors.
  JOURNAL   eLife. 2022 Dec 28;11:e81856. doi: 10.7554/eLife.81856.
  PUBMED    36576240
REFERENCE   2  (bases 1 to 9527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2022-12-28"; volume: "11:e81856. doi"; pages: " 
            10.7554/eLife.81856"
COMMENT     Sequence Label: pJR103
FEATURES             Location/Qualifiers
     source          1..9527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(71..90)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        262..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        643..841
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             859..1039
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1086..1211
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1708..1941
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2126..2170
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2468..2585
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2838..2871)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2875..2895
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        3771..4949
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4002..4940
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4897..4917
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4961..5557
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4961..4980)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5457..5477
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5567..5620
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5633..6331
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     protein_bind    complement(6351..6384)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6440..7028
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6493..6513)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6911..6922)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7031..7047)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7032..7048)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7557..7737
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7799..8387)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7879..7898)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8558..9349,9350..9418))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9181..9200
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9419..9523)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
       61 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
      121 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      181 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      241 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      781 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
      901 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
      961 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     1021 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     1081 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     1141 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     1201 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     1261 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     1321 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     1381 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     1441 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     1501 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct
     1561 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     1621 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     1681 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     1741 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     1801 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     1861 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     1921 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     1981 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     2041 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     2101 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     2161 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     2221 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     2281 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     2341 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     2401 tcgattagtg aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca
     2461 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     2521 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     2581 attttcgggt ttattacagg gacagcagag atccagtttg gttagtaccg ggcccgctct
     2641 agagatccga cgcgccatct ctaggcccgc gccggccccc tcgcacggac ttgtgggaga
     2701 agctcggcta ctcccctgcc ccggttaatt tgcatataat atttcctagt aactatagag
     2761 gcttaatgtg cgataaaaga cagataatct gttcttttta atactagcta cattttacat
     2821 gataggcttg gatttctata acttcgtata gcatacatta tacgaagtta taaacagcac
     2881 aaaaggaaac tcaccctaac tgtaaagtaa ttgtgtgttt tgagactata agtatccctt
     2941 ggagaaccac cttgttggac caggatgggc accacccgtt taagagctaa gctggaaaca
     3001 gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     3061 tgcttttttt ctcgagtact aggatccatt aggcggccgc cgggaggtgg tccctgcagt
     3121 tacgccaatg ataacccccg ccagaaaaat cttagtagcc ttcccttttt gttttccgtg
     3181 ccccaactcg gcggattgac tcggcccctt ccggaaacac ccgaatcaac ttctagtcaa
     3241 attattgttc acgccgcaat gacccacccc tggcccgcgt ctgtggaact gacccctggt
     3301 gtacaggaga gttcgctgct gaaagtggtc ccaaaggggt actagttttt aagctcccaa
     3361 ctccccctcc cccagcgtct ggaggattcc acaccctcgc accgcagggg cgaggaagtg
     3421 ggcggagtcc ggttttggcg ccagccgctg aggctgccaa gcagaaaagc caccgctgag
     3481 gagactccgg tcactgtcct cgccccgcct cccccttccc tccccttggg gaccaccggg
     3541 cgccacgccg cgaacggtaa gtgccgcggt cgtcggcgcc tccgccctcc ccctagggcc
     3601 ccaattccca gcgggcgcgg cgcgcggccc ctccccccgc cgggcgcgcg cccgctgccc
     3661 cgcccttcgt ggccgcccgg cgtgggcggt gccacccctc cccccggcgg ccccgcgcgc
     3721 agctcccggc tccctccccc ttcggatgtg gcttgagctg taggcgcgga gatgcatgtg
     3781 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     3841 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     3901 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     3961 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
     4021 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
     4081 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
     4141 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
     4201 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
     4261 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
     4321 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
     4381 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
     4441 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
     4501 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     4561 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     4621 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     4681 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcgc
     4741 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     4801 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     4861 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     4921 gtttttttct tccatttcag gtgtcgtgag ctagcccacc atgaccgagt acaagcccac
     4981 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt
     5041 cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac
     5101 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     5161 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     5221 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     5281 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     5341 cgtcggcgtg tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     5401 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     5461 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     5521 accgcgcacc tggtgcatga cccgcaagcc cggtgccgga tcgggagagg gcagaggaag
     5581 tctgctaaca tgcggtgacg tcgaggagaa tcctggccca ccggtcgcca ccagcgagct
     5641 gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt
     5701 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     5761 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta
     5821 cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt
     5881 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
     5941 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     6001 gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac
     6061 cgagaccctg taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct
     6121 cgtgggcggg agccatctga tcgcaaacat caagaccaca tatagatcca agaaacccgc
     6181 taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga
     6241 ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct
     6301 ccctagcaaa ctggggcaca agcttaatta agaattcgtc gagggaccta ataacttcgt
     6361 atagcataca ttatacgaag ttatacatgt ttaagggttc cggttccact aggtacaatt
     6421 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6481 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6541 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6601 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6661 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6721 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6781 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     6841 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6901 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6961 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7021 ctccccgcat cgataccgtc gacctcgatc gagacctaga aaaacatgga gcaatcacaa
     7081 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     7141 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     7201 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7261 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     7321 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     7381 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     7441 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     7501 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     7561 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     7621 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     7681 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca
     7741 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7801 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7861 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7921 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7981 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8041 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8101 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8161 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8221 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8281 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8341 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8401 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8461 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8521 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8581 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8641 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     8701 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8761 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     8821 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     8881 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     8941 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9001 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9061 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9121 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9181 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9241 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9301 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9361 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9421 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9481 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcaca
//
