LOCUS       sequence-374825-        9844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 
            constant region, and UCOE EF1alpha driving PURO-GFP marker 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9844)
  AUTHORS   Replogle JM, Bonnar JL, Pogson AN, Liem CR, Maier NK, Ding Y, 
            Russell BJ, Wang X, Leng K, Guna A, Norman TM, Pak RA, Ramos DM, 
            Ward ME, Gilbert LA, Kampmann M, Weissman JS, Jost M
  TITLE     Maximizing CRISPRi efficacy and accessibility with dual-sgRNA 
            libraries and optimal effectors.
  JOURNAL   eLife. 2022 Dec 28;11:e81856. doi: 10.7554/eLife.81856.
  PUBMED    36576240
REFERENCE   2  (bases 1 to 9844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2022-12-28"; volume: "11:e81856. doi"; pages: " 
            10.7554/eLife.81856"
COMMENT     Sequence Label: pJR107
FEATURES             Location/Qualifiers
     source          1..9844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    88..109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(124..129,130..147,148..154)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    162..178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     167..189
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     186..202
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     186..202
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     221..241
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        223..241
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        269..495
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             496..676
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    723..848
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1341..1574
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1759..1803
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1984..2101
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2843..4021
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3074..4012
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3969..3989
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     complement(4033..4052)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             4639..4692
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    5470..6058
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5523..5543)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5941..5952)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6414..6447)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     6451..6471
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     LTR             6699..6879
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6957..7078
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6994..7013)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7048..7067
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7097..7232
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7252..7271)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7253..7271)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7281..7298)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7281..7297)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7290..7312)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7439..7894
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7526..7545)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7736..7757
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7920..8024
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8025..8093,8094..8885)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8243..8262)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9056..9644
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9545..9564
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9798..9815
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
       61 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
      121 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
      181 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
      241 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
      301 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
      361 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
      421 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
      481 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
      541 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
      601 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
      661 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
      721 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
      781 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
      841 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
      901 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
      961 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     1021 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     1081 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     1141 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     1201 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     1261 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     1321 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     1381 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     1441 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     1501 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     1561 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     1621 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     1681 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     1741 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     1801 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     1861 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     1921 aggcagggat attcaccatt atcgtttcag aatcttgaga caaatggcag tattcatcca
     1981 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2041 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     2101 tcgggtttat tacagggaca gcagagatcc actttggcgc cggccgagta ctaggatcca
     2161 ttaggcggcc gccgggaggt ggtccctgca gttacgccaa tgataacccc cgccagaaaa
     2221 atcttagtag ccttcccttt ttgttttccg tgccccaact cggcggattg actcggcccc
     2281 ttccggaaac acccgaatca acttctagtc aaattattgt tcacgccgca atgacccacc
     2341 cctggcccgc gtctgtggaa ctgacccctg gtgtacagga gagttcgctg ctgaaagtgg
     2401 tcccaaaggg gtactagttt ttaagctccc aactccccct cccccagcgt ctggaggatt
     2461 ccacaccctc gcaccgcagg ggcgaggaag tgggcggagt ccggttttgg cgccagccgc
     2521 tgaggctgcc aagcagaaaa gccaccgctg aggagactcc ggtcactgtc ctcgccccgc
     2581 ctcccccttc cctccccttg gggaccaccg ggcgccacgc cgcgaacggt aagtgccgcg
     2641 gtcgtcggcg cctccgccct ccccctaggg ccccaattcc cagcgggcgc ggcgcgcggc
     2701 ccctcccccc gccgggcgcg cgcccgctgc cccgcccttc gtggccgccc ggcgtgggcg
     2761 gtgccacccc tccccccggc ggccccgcgc gcagctcccg gctccctccc ccttcggatg
     2821 tggcttgagc tgtaggcgcg gagatgcatg tgcccgtcag tgggcagagc gcacatcgcc
     2881 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg
     2941 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     3001 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     3061 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
     3121 tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga
     3181 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
     3241 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
     3301 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
     3361 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
     3421 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     3481 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     3541 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     3601 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     3661 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     3721 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     3781 ggcgccgtcc aggcacctcg attagttctc gcgcttttgg agtacgtcgt ctttaggttg
     3841 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     3901 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     3961 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     4021 agctagccca ccatgaccga gtacaagccc accgttcgcc tggccacaag agatgacgtg
     4081 cctcgcgccg tgagaacttt ggctgcggcg ttcgccgatt atccagcaac caggcacaca
     4141 gtggaccctg atagacacat cgagcgagtg actgagctcc aggaactgtt tctgacacgg
     4201 gttggtctgg atatcggaaa agtgtgggtc gctgacgatg gggccgccgt ggcagtctgg
     4261 acaaccccag aatccgtgga ggccggagca gtattcgccg agataggccc ccgcatggcg
     4321 gagctttcgg ggtcaaggct ggctgcacag cagcaaatgg agggcctact cgctccacac
     4381 cggcctaaag aacccgcctg gttcctagcc actgttggcg tgtcaccgga ccatcaggga
     4441 aaagggctgg gctctgctgt ggtgctgcct ggtgtcgagg ctgcggagag ggccggagtg
     4501 ccggcttttc ttgaaactag cgcacctagg aacttacctt tctacgagcg tctgggattt
     4561 accgtcacag ccgacgtgga ggttccagag gggccccgga cttggtgtat gacacggaaa
     4621 ccgggcgcag gttctgggga gggtaggggg tcacttctga cgtgcgggga tgtggaagaa
     4681 aaccccggcc caccagtagc aacgatggtc agtaagggcg aggaactttt tacaggggtc
     4741 gttcccattc tggttgaatt ggatggcgat gtgaacggac ataaatttag cgtgtccggc
     4801 gaaggagaag gtgatgctac ctacggaaag cttacgttga agtttatttg cacgaccggt
     4861 aagctgcccg taccatggcc aacattggtg accactctga cctatggagt gcagtgtttc
     4921 agccggtacc ccgaccacat gaaacaacac gacttcttca agtctgccat gcctgaaggt
     4981 tatgtccagg aacgtaccat cttttttaaa gacgacggaa attataagac ccgagccgag
     5041 gtaaagtttg agggcgacac tctcgtcaac cgcatagaat tgaaggggat tgacttcaaa
     5101 gaagatggca acattctagg ccataaatta gagtataatt acaacagcca caacgtttac
     5161 atcatggctg acaaacagaa gaatggcatc aaggcaaatt tcaagataag acacaatatc
     5221 gaggacggga gtgtccaact ggccgaccat tatcagcaga atacacctat tggcgacgga
     5281 cctgtcctcc tccccgacaa tcattacctg agtactcaat ccgcactctc caaagatccc
     5341 aacgagaaga gagatcacat ggtattactg gagttcgtga cagctgccgg gatcaccctc
     5401 ggcatggatg aactctacaa gtgagaattc gtcgagggac ctaataacta ttaaacgcgt
     5461 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5521 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5581 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5641 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5701 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5761 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     5821 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     5881 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5941 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6001 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     6061 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6121 aggggggact ggaagggcta attcactccc aacgaagaca agatttcacc attatcgttt
     6181 cagacccacc tcccaacccc gaggggaccc agatctagag atccgacgcg ccatctctag
     6241 gcccgcgccg gccccctcgc acggacttgt gggagaagct cggctactcc cctgccccgg
     6301 ttaatttgca tataatattt cctagtaact atagaggctt aatgtgcgat aaaagacaga
     6361 taatctgttc tttttaatac tagctacatt ttacatgata ggcttggatt tctataactt
     6421 cgtatagcat acattatacg aagttataaa cagcacaaaa ggaaactcac cctaactgta
     6481 aagtaattgt gtgttttgag actataagta tcccttggag aaccaccttg ttggaccagg
     6541 atgggcacca cccgtttaag agctaagctg gaaacagcat agcaagttta aataaggcta
     6601 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttctcg agaagcttgg
     6661 cgtaactaga tcttgagaca ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     6721 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6781 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6841 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     6901 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     6961 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7021 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7081 atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7141 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     7201 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     7261 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     7321 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     7381 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     7441 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     7501 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     7561 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7621 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     7681 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7741 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     7801 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7861 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     7921 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7981 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     8041 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     8101 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     8161 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     8221 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     8281 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     8341 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     8401 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     8461 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     8521 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     8581 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     8641 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     8701 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     8761 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     8821 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     8881 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8941 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     9001 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     9061 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     9121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     9181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     9241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     9301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     9361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     9421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     9481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     9661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     9721 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     9781 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     9841 aacc
//
