LOCUS       sequence-374833-       10279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A-to-G base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10279)
  AUTHORS   Abdullah, Wang P, Han T, Liu W, Ren W, Wu Y, Xiao Y
  TITLE     Adenine Base Editing System for Pseudomonas and Prediction Workflow 
            for Protein Dysfunction via ABE.
  JOURNAL   ACS Synth Biol. 2022 Apr 15;11(4):1650-1657. doi: 
            10.1021/acssynbio.2c00066. Epub 2022 Apr 7.
  PUBMED    35389616
REFERENCE   2  (bases 1 to 10279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00066"; journalName: "ACS Synth Biol"; date: 
            "2022-04-15- 15"; volume: "11"; issue: "4"; pages: "1650-1657"
COMMENT     Sequence Label: pnxABE-flgn
FEATURES             Location/Qualifiers
     source          1..10279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..23,24..30)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     misc_RNA        51..126
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             918..5018
                     /codon_start=1
                     /product="catalytically dead Streptococcus pyogenes Cas9 
                     mutant with broad PAM compatibility (Hu et al., 2018)"
                     /label=dxCas9(3.7)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFIQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             918..5018
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 mutant with broad PAM
                     compatibility (Hu et al., 2018)"
                     /label=xCas9-3.7
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFIQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     promoter        join(5143..5148,5149..5165,5166..5171)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5360..5893
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      6191..6542
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      7560..7654
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      7742..8330
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8231..8250
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8421..8440
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(join(8479..9813,9814..9900))
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
ORIGIN
        1 ttgacaatta atcatccggc tcgtataatg cagcaccttg ctgcggcgtt gttttagagc
       61 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
      121 cggtgctttt tttgagatct gtccataccc atggtctaga atgctcgagg catcaacgac
      181 aagccccggc tatcccgata gccggggctt tgtcttgacg gtgtacaaga acctctttac
      241 tattttgtac cccgaaaatt ggcagggcgc acgctctgtc atttcgtttg tgtgtcgtaa
      301 gacaatcagt ggagctatag acgtctgagg tggagttttc ccacgagtac tggatgagac
      361 atgccctgac cctggccaag agggcacggg atgagaggga ggtgcctgtg ggagccgtgc
      421 tggtgctgaa caatagagtg atcggcgagg gctggaacag agccatcggc ctgcacgacc
      481 caacagccca tgccgaaatt atggccctga gacagggcgg cctggtcatg cagaactaca
      541 gactgattga cgccaccctg tacgtgacat tcgagccttg cgtgatgtgc gccggcgcca
      601 tgatccactc taggatcggc cgcgtggtgt ttggcgtgag gaactcaaaa agaggcgccg
      661 caggctccct gatgaacgtg ctgaactacc ccggcatgaa tcaccgcgtc gaaattaccg
      721 agggaatcct ggcagatgaa tgtgccgccc tgctgtgcga tttctatcgg atgcctagac
      781 aggtgttcaa tgctcagaag aaggcccaga gctccatcaa ctccggagga tctagcggag
      841 gctcctctgg ctctgagaca cctggcacaa gcgagagcgc aacacctgaa agcagcgggg
      901 gcagcagcgg ggggtcagac aagaagtact ccattgggct cgctatcggc acaaacagcg
      961 tcggctgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc aaagttctgg
     1021 gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg ttcgactccg
     1081 gggagaccgc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat acccgcagaa
     1141 agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag gtggatgact
     1201 ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag cacgagcgcc
     1261 acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac ccaaccatat
     1321 atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg ttgatctatc
     1381 tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg gacctgaacc
     1441 cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac aatcagcttt
     1501 tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg agcgctaggc
     1561 tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag aagaagaacg
     1621 gcctgtttgg taatcttatc gccctgtccc tcgggctgac ccccaacttt aaatctaact
     1681 tcgacctggc cgaagatacc aagcttcaac tgagcaaaga cacctacgat gatgatctcg
     1741 acaatctgct ggcccagatc ggcgaccagt acgcagacct ttttttggcg gcaaagaacc
     1801 tgtcagacgc cattctgctg agtgatattc tgcgagtgaa cacggagatc accaaagctc
     1861 cgctgagcgc tagtatgatc aagctctatg atgagcacca ccaagacttg actttgctga
     1921 aggcccttgt cagacagcaa ctgcctgaga agtacaagga aattttcttc gatcagtcta
     1981 aaaatggcta cgccggatac attgacggcg gagcaagcca ggaggaattt tacaaattta
     2041 ttaagcccat cttggaaaaa atggacggca ccgaggagct gctggtaaag cttaacagag
     2101 aagatctgtt gcgcaaacag cgcactttcg acaatggaat catcccccac cagattcacc
     2161 tgggcgaact gcacgctatc ctcaggcggc aagaggattt ctaccccttt ttgaaagata
     2221 acagggaaaa gattgagaaa atcctcacat ttcggatacc ctactatgta ggccccctcg
     2281 cccggggaaa ttccagattc gcgtggatga ctcgcaaatc agaagagacc atcactccct
     2341 ggaacttcga gaaagtcgtg gataaggggg cctctgccca gtccttcatc gaaaggatga
     2401 ctaactttga taaaaatctg cctaacgaaa aggtgcttcc taaacactct ctgctgtacg
     2461 agtacttcac agtttataac gagctcacca aggtcaaata cgtcacagaa gggatgagaa
     2521 agccagcatt cctgtctgga gatcagaaga aagctattgt ggacctcctc ttcaagacga
     2581 accggaaagt taccgtgaaa cagctcaaag aagactattt caaaaagatt gaatgtttcg
     2641 actctgttga aatcagcgga gtggaggatc gcttcaacgc atccctggga acgtatcacg
     2701 atctcctgaa aatcattaaa gacaaggact tcctggacaa tgaggagaac gaggacattc
     2761 ttgaggacat tgtcctcacc cttacgttgt ttgaagatag ggagatgatt gaagaacgct
     2821 tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa gcagctcaag aggcgccgat
     2881 atacaggatg ggggcggctg tcaagaaaac tgatcaatgg gatccgagac aagcagagtg
     2941 gaaagacaat cctggatttt cttaagtccg atggatttgc caaccggaac ttcattcagt
     3001 tgatccatga tgactctctc acctttaagg aggacatcca gaaagcacaa gtttctggcc
     3061 agggggacag tcttcacgag cacatcgcta atcttgcagg tagcccagct atcaaaaagg
     3121 gaatactgca gaccgttaag gtcgtggatg aactcgtcaa agtaatggga aggcataagc
     3181 ccgagaatat cgttatcgag atggcccgag agaaccaaac cacccagaag ggacagaaga
     3241 acagtaggga aaggatgaag aggattgaag agggtataaa agaactgggg tcccaaatcc
     3301 ttaaggaaca cccagttgaa aacacccagc ttcagaatga gaagctctac ctgtactacc
     3361 tgcagaacgg cagggacatg tacgtggatc aggaactgga catcaatcgg ctctccgact
     3421 acgacgtgga ccatatcgtg ccccagtctt ttctcaaaga tgattctatt gataataaag
     3481 tgttgacaag atccgataaa aacagaggga agagtgataa cgtcccctca gaagaagttg
     3541 tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc caaactgatc acacaacgga
     3601 agttcgataa tctgactaag gctgaacgag gtggcctgtc tgagttggat aaagccggtt
     3661 tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa gcacgtggcc caaattctcg
     3721 attcacgcat gaacaccaag tacgatgaaa atgacaaact gattcgagag gtgaaagtta
     3781 ttactctgaa gtctaagctg gtctcagatt tcagaaagga ctttcagttt tataaggtga
     3841 gagagatcaa caattaccac catgcgcatg atgcctacct gaatgcagtg gtaggcactg
     3901 cacttatcaa aaaatatccc aagcttgaat ctgaatttgt ttacggagac tataaagtgt
     3961 acgatgttag gaaaatgatc gcaaagtctg agcaggaaat aggcaaggcc accgctaagt
     4021 acttctttta cagcaatatt atgaattttt tcaagaccga gattacactg gccaatggag
     4081 agattcggaa gcgaccactt atcgaaacaa acggagaaac aggagaaatc gtgtgggaca
     4141 agggtaggga tttcgcgaca gtccggaagg tcctgtccat gcctcaggtg aacatcgtta
     4201 aaaagaccga agtacagacc ggaggtttct ccaaggaaag tatcctcccg aaaaggaaca
     4261 gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa gaaatacggc ggattcgatt
     4321 ctcctacagt cgcttacagt gtactggttg tggccaaagt ggagaaaggg aagtctaaaa
     4381 aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat ggagcgatca agcttcgaaa
     4441 aaaaccccat cgactttctc gaggcgaaag gatataaaga ggtcaaaaaa gacctcatca
     4501 ttaagcttcc caagtactct ctctttgagc ttgaaaacgg ccggaaacga atgctcgcta
     4561 gtgcgggcgt gctgcagaaa ggtaacgagc tggcactgcc ctctaaatac gttaatttct
     4621 tgtatctggc cagccactat gaaaagctca aagggtctcc cgaagataat gagcagaagc
     4681 agctgttcgt ggaacaacac aaacactacc ttgatgagat catcgagcaa ataagcgaat
     4741 tctccaaaag agtgatcctc gccgacgcta acctcgataa ggtgctttct gcttacaata
     4801 agcacaggga taagcccatc agggagcagg cagaaaacat tatccacttg tttactctga
     4861 ccaacttggg cgcgcctgca gccttcaagt acttcgacac taccatagac agaaagcggt
     4921 acacctctac aaaggaggtc ctggacgcca cactgattca tcagtcaatt acggggctct
     4981 atgaaacaag aatcgacctc tctcagctcg gtggagactg atggctggtt ggcgtactgt
     5041 tgtggtaaat acccatgggt atggacagtt ttcccgaata ggaacttcgg aataggaact
     5101 tcaagatccc ctgattccct ttgtcaacag caatggatcg aattgacata agcctgttcg
     5161 gttcgtaaac tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc
     5221 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
     5281 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     5341 atgttatgga gcagcaacga tgttacgcag cagcaacgat gttacgcagc agggcagtcg
     5401 ccctaaaaca aagttaggtg gctcaagtat gggcatcatt cgcacatgta ggctcggccc
     5461 tgaccaagtc aaatccatgc gggctgctct tgatcttttc ggtcgtgagt tcggagacgt
     5521 agccacctac tcccaacatc agccggactc cgattacctc gggaacttgc tccgtagtaa
     5581 gacattcatc gcgcttgctg ccttcgacca agaagcggtt gttggcgctc tcgcggctta
     5641 cgttctgccc aagtttgagc agccgcgtag tgagatctat atctatgatc tcgcagtctc
     5701 cggcgagcac cggaggcagg gcattgccac cgcgctcatc aatctcctca agcatgaggc
     5761 caacgcgctt ggtgcttatg tgatctacgt gcaagcagat tacggtgacg atcccgcagt
     5821 ggctctctat acaaagttgg gcatacggga agaagtgatg cactttgata tcgacccaag
     5881 taccgccacc taacaattcg ttcaagccga gatcagcttc ccggccgcgg agttgttcgg
     5941 taaattgtca caacgccgcg gccaattcga gagttttcgt tccactgagc gtcagacccc
     6001 aattacacgc cactggctgt gcttgctggg gtgacggtgg caacggtggc ggccttgctg
     6061 ggctatcgcg ttggaaagaa acgagggaaa ggggactgat aaaccggtct tagcccctcc
     6121 ccttggtgtc caaccgctct gtaggcctct caggcgccgc tggtgccgct ggttggacgc
     6181 caagggtgaa tccgcctcga taccctgatt actcgcttcc tgcgccctct caggcggcga
     6241 taggggactg gtaaaacggg gattgcccag acgcctcccc cgccccttca ggggcacaaa
     6301 tgcggcccca acggggccac gtagtggtgc gttttttgcg tttccaccct tttcttcctt
     6361 ttccctttta aaccttttag gacgtctaca ggccacgtaa tccgtggcct gtagagttta
     6421 aaaagggacg gatttgttgc cattaaggga cggatttgtt gttaagaagg gacggatttg
     6481 ttgttgtaaa gggacggatt tgttgtattg tgggacgcag atacagtgtc cccttataca
     6541 caaggaatgt cgaacgtggc ctcaccccca atggtttaca aaagcaatgc cctggtcgag
     6601 gccgcgtatc gcctcagtgt tcaggaacag cggatcgttc tggcctgtat tagccaggtg
     6661 aagaggagcg agcctgtcac cgatgaagtg atgtattcag tgacggcgga ggacatagcg
     6721 acgatggcgg gtgtccctat cgaatcttcc tacaaccagc tcaaagaagc ggccctgcgc
     6781 ctgaaacggc gggaagtccg gttaacccaa gagcccaatg gcaaggggaa aagaccgagt
     6841 gtgatgatta ccggctgggt gcaaacaatc atctaccggg agggtgaggg ccgtgtagaa
     6901 ctcaggttca ccaaagacat gctgccgtac ctgacggaac tcaccaaaca gttcaccaaa
     6961 tacgccttgg ctgacgtggc caagatggac agcacccacg cgatcaggct ttacgagctg
     7021 ctcatgcaat gggacagcat cggccagcgc gaaatagaaa ttgaccagct gcgaaagtgg
     7081 tttcaactgg aaggccggta tccctcgatc aaggacttca agttgcgagt gcttgatcca
     7141 gccgtgacgc agatcaacga gcacagcccg ctacaggtgg agtgggcgca gcgaaagacc
     7201 gggcgcaagg tcacacatct gttgttcagt tttggaccga agaagcccgc caaggcggtg
     7261 ggtaaggccc cagcgaagcg caaggccggg aagatttcag atgctgagat cgcgaaacag
     7321 gctcgccctg gtgagacatg ggaagcggcc cgcgctcgac taacccagat gccgcttgcg
     7381 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     7441 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     7501 tgctgataaa tctggagccg gtgagcgtgg cgggactctg gggttcgaga gctcgcttgg
     7561 actcctgttg atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct
     7621 cggttgccgc cgggcgtttt ttattggtga gaatccaagc actagtcatg accaaaatcc
     7681 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     7741 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7801 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     7861 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     7921 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     7981 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     8041 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     8101 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     8161 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     8221 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     8281 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     8341 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     8401 cgttgtcgac agagcccggg cggcatcaga gcagattgta ctgagagtgc accataatcg
     8461 gcattttctt ttgcgttttt atttgttaac tgttaattgt ccttgttcaa ggatgctgtc
     8521 tttgacaaca gatgttttct tgcctttgat gttcagcagg aagctaggcg caaacgttga
     8581 ttgtttgtct gcgtagaatc ctctgtttgt catatagctt gtaatcacga cattgtttcc
     8641 tttcgcttga ggtacagcga agtgtgagta agtaaaggtt acatcgttag gatcaagatc
     8701 catttttaac acaaggccag ttttgttcag cggcttgtat gggccagtta aagaattaga
     8761 aacataacca agcatgtaaa tatcgttaga cgtaatgccg tcaatcgtca tttttgatcc
     8821 gcgggagtca gtgaacagat accatttgcc gttcatttta aagacgttcg cgcgttcaat
     8881 ttcatctgtt actgtgttag atgcaatcag cggtttcatc acttttttca gtgtgtaatc
     8941 atcgtttagc tcaatcatac cgagagcgcc gtttgctaac tcagccgtgc gttttttatc
     9001 gctttgcaga agtttttgac tttcttgacg gaagaatgat gtgcttttgc catagtatgc
     9061 tttgttaaat aaagattctt cgccttggta gccatcttca gttccagtgt ttgcttcaaa
     9121 tactaagtat ttgtggcctt tatcttctac gtagtgagga tctctcagcg tatggttgtc
     9181 gcctgagctg tagttgcctt catcgatgaa ctgctgtaca ttttgatacg tttttccgtc
     9241 accgtcaaag attgatttat aatcctctac accgttgatg ttcaaagagc tgtctgatgc
     9301 tgatacgtta acttgtgcag ttgtcagtgt ttgtttgccg taatgtttac cggagaaatc
     9361 agtgtagaat aaacggattt ttccgtcaga tgtaaatgtg gctgaacctg accattcttg
     9421 tgtttggtct tttaggatag aatcatttgc atcgaatttg tcgctgtctt taaagacgcg
     9481 gccagcgttt ttccagctgt caatagaagt ttcgccgact ttttgataga acatgtaaat
     9541 cgatgtgtca tccgcatttt taggatctcc ggctaatgca aagacgatgt ggtagccgtg
     9601 atagtttgcg acagtgccgt cagcgttttg taatggccag ctgtcccaaa cgtccaggcc
     9661 ttttgcagaa gagatatttt taattgtgga cgaatcgaac tcaggaactt gatatttttc
     9721 atttttttgc tgttcaggga tttgcagcat atcatggcgt gtaatatggg aaatgccgta
     9781 tgtttcctta tatggctttt ggttcgtttc tttcgcaaac gcttgagttg cgcctcctgc
     9841 cagcagtgcg gtagtaaagg ttaatactgt tgcttgtttt gcaaactttt tgatgttcat
     9901 cgttcatgtc tcctttttta tgtactgtgt tagcggtctg cttcttccag ccctcctgtt
     9961 tgaagatggc aagttagtta cgcacaataa aaaaagacct aaaatatgta aggggtgacg
    10021 ccaaagtata cactttgccc tttacacatt ttaggtcttg cctgctttat cagtaacaaa
    10081 cccgcgcgat ttacttttcg acctcattct attagactct cgtttggatt gcaactggtc
    10141 tattttcctc ttttgtttga tagaaaatca taaaaggatt tgcagactac gggcctaaag
    10201 aactaaaaaa tctatctgtt tcttttcatt ctctgtattt tttatagttt ctgttgcatg
    10261 ggcataaagt tgccttaag
//
