LOCUS       sequence-374834-       12929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression with FACS-selectable marker (extracellular 
            part of NGFR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12929)
  AUTHORS   van_de_Vosse E, Verhard EM, de Paus RA, Platenburg GJ, van Deutekom 
            JC, Aartsma-Rus A, van Dissel JT
  TITLE     Antisense-mediated exon skipping to correct IL-12Rbeta1 deficiency 
            in T cells.
  JOURNAL   Blood. 2009 May 7;113(19):4548-55. doi: 
            10.1182/blood-2008-12-196220. Epub 2009 Mar 3.
  PUBMED    19258592
REFERENCE   2  (bases 1 to 12929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2008-12-196220"; journalName: "Blood"; date: 
            "2009-05-7- 7"; volume: "113"; issue: "19"; pages: "4548-55"
COMMENT     Sequence Label: pLZRS-IRES-deltaNGFR
FEATURES             Location/Qualifiers
     source          1..12929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             121..590
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    655..1012
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1077..1493
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1423..1445
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1461..1477
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1503..1877
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1815..1831
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     1911..1927
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1912..1928
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(1964..2538,2539..2541,2542..2550)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2131..2148)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2358..2377
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             3583..4052
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4182..4199)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4353..4941)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4433..4452)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5064..5198
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5088..5107)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5142..5161
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5428..6027)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5511..5531)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6008..6027
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(6110..6609)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6158..6177)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     6551..6574
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             7038..8963
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGXGGXGXGGGXGXGGGXGGXGGXGXGGGXGXGGGXGGXGXGGGX
                     GGXGGXGXGGGAGXGGGXGGXGXGGGXGGXGGXGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGXGGXGGXGXGGXGGXGXGGXGGXGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     rep_origin      9265..11054
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(join(11548..12339,12340..12408))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12171..12190
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12409..12513)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag
      121 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      241 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      301 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      361 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      421 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
      481 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      541 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      601 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      661 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      721 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      781 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
      841 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
      901 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
      961 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
     1021 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggcc
     1081 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1141 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1201 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1261 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1321 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1381 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1441 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     1501 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
     1561 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     1621 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     1681 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     1741 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     1801 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     1861 gaccatcctc tagactgccg gatccattta aattcgaatt cctgcaggcc tcgaggtcga
     1921 cggtatcgat aagcttgata tcgaattaat tccgcccccc ccccccntct ccctcccccc
     1981 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     2041 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     2101 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     2161 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     2221 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     2281 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     2341 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     2401 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     2461 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     2521 gaaaaacacg atgataatat ggccacaacc atgggggcag gtgccaccgg ccgcgccatg
     2581 gacgggccgc gcctgctgct gttgctgctt ctgggggtgt cccttggagg tgccaaggag
     2641 gcatgcccca caggcctgta cacacacagc ggtgagtgct gcaaagcctg caacctgggc
     2701 gagggtgtgg cccagccttg tggagccaac cagaccgtgt gtgagccctg cctggacagc
     2761 gtgacgttct ccgacgtggt gagcgcgacc gagccgtgca agccgtgcac cgagtgcgtg
     2821 gggctccaga gcatgtcggc gccgtgcgtg gaggccgacg acgccgtgtg ccgctgcgcc
     2881 tacggctact accaggatga gacgactggg cgctgcgagg cgtgccgcgt gtgcgaggcg
     2941 ggctcgggcc tcgtgttctc ctgccaggac aagcagaaca ccgtgtgcga ggagtgcccc
     3001 gacggcacgt attccgacga ggccaaccac gtggacccgt gcctgccctg caccgtgtgc
     3061 gaggacaccg agcgccagct ccgcgagtgc acacgctggg ccgacgccga gtgcgaggag
     3121 atccctggcc gttggattac acggtccaca cccccagagg gctcggacag cacagccccc
     3181 agcacccagg agcctgaggc acctccagaa caagacctca tagccagcac ggtggcaggt
     3241 gtggtgacca cagtgatggg cagctcccag cccgtggtga cccgaggcac caccgacaac
     3301 ctcatccctg tctattgctc catcctggct gctgtggttg tgggccttgt ggcctacata
     3361 gccttcaaga ggtggaacag ggggatactc tagagcggcc gccagcacag tggtcgacga
     3421 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     3481 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga
     3541 gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca
     3601 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg
     3661 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     3721 cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc
     3781 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
     3841 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
     3901 tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa
     3961 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
     4021 gtgattgact acccgtcagc gggggtcttt cacatgcagc atgtatcaaa attaatttgg
     4081 ttttttttct taagtattta cattaaatgg ccatagttgc attaatgaat cggccaacgc
     4141 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     4201 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     4261 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4321 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4381 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4441 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4501 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4561 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4621 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4681 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4741 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     4801 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4861 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     4921 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     4981 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     5041 tagatccttt tgcggccgaa ttagatccag acatgataag atacattgat gagtttggac
     5101 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     5161 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     5221 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     5281 aatgtggtat ggctgattat gatcctctag agtcggtggg cctcgggggc gggtgcgggg
     5341 tcggcggggc cgccccgggt ggcttcggtc ggagccatgg ggtcgtgcgc tcctttcggt
     5401 cgggcgctgc gggtcgtggg gcgggcgtca ggcaccgggc ttgcgggtca tgcaccaggt
     5461 gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa
     5521 ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc
     5581 ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac
     5641 gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc
     5701 catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc
     5761 ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc
     5821 gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg
     5881 cagctcggtg acccgctcga tgtggcggtc cgggtcgacg gtgtggcgcg tggcggggta
     5941 gtcggcgaac gcggcggcga gggtgcgtac ggcccggggg acgtcgtcgc gggtggcgag
     6001 gcgcaccgtg ggcttgtact cggtcatgga aggtcgtctc cttgtgaggg gtcaggggcg
     6061 tgggtcaggg gatggtggcg gcaccggtcg tggcggccga cctgcaggtc gaaaggcccg
     6121 gagatgagga agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg
     6181 ggcctccgga ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg agcccgcccc
     6241 cggacccacc ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc tgctattggc
     6301 cgctgcccca aaggcctacc cgcttccatt gctcagcggt gctgtccatc tgcacgagac
     6361 tagtgagacg tgctacttcc atttgtcacg tcctgcacga cgcgagctgc ggggcggggg
     6421 ggaacttcct gactagggga ggagtagaag gtggcgcgaa ggggccacca aagaacggag
     6481 ccggttggcg cctaccggtg gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc
     6541 gccaagtgcc cagcggggct gctaaagcgc atgctccaga ctgccttggg aaaagcgcct
     6601 cccctacccg gtagaattaa ttctcatgtt tgacagctta tcatcgataa gctgatcctc
     6661 acaggccgca cccagctttt cttccgttgc cccagtagca tctctgtctg gtgaccttga
     6721 agaggaagag gaggggtccc gagaatcccc atccctaccg tccagcaaaa agggggacga
     6781 ggaatttgag gcctggcttg aggctcagga cgcaaatctt gaggatgttc agcgggagtt
     6841 ttccgggctg cgagtaattg gtgatgagga cgaggatggt tcggaggatg gggaattttc
     6901 agacctggat ctgtctgaca gcgaccatga aggggatgag ggtggggggg ctgttggagg
     6961 gggcaggagt ctgcactccc tgtattcact gagcgtcgtc taataaagat gtctattgat
     7021 ctcttttagt gtgaatcatg tctgacgagg ggccaggtac aggacctgga aatggcctag
     7081 gagagaaggg agacacatct ggaccagaag gctccggcgg cagtggacct caaagaagag
     7141 ggggtgataa ccatggacga ggacggggaa gaggacgagg acgaggaggc ggaagaccag
     7201 gagccccggg cggctcagga tcagggccaa gacatagaga tggtgtccgg agaccccaaa
     7261 aacgtccaag ttgcattggc tgcaaaggga cccacggtgg aacaggagca ggagcaggag
     7321 cgggaggggc aggagcagga ggggcaggag caggaggagg ggcaggagca ggaggagggg
     7381 caggaggggc aggaggggsa ggaggggsag gagsaggagg aggggsagga gsaggaggag
     7441 gggsaggagg rgsaggaggr gsaggagsag gaggaggrgs aggagsagga ggaggggsag
     7501 gaggggsagg agsaggagga ggggsaggag gggsaggagg ggsaggagsa ggaggagggg
     7561 caggagsagg aggaggggsa ggaggggsag gagsaggagg aggggsagga ggggsaggag
     7621 gggsaggagc aggaggaggg gcaggagcag gaggggcagg aggggcagga ggggcaggag
     7681 caggaggggc aggagcagga ggaggggcag gaggggcagg aggggcagga gcaggagggg
     7741 caggagcagg aggggcagga gcaggagggg caggagsagg aggggsagga ggggsaggag
     7801 saggaggggn aggaggggna ggagsaggag gggsaggagg ggsaggagca ggaggagggg
     7861 caggaggggc aggagcagga ggaggggcag gaggggcagg agcaggaggg gcaggagggg
     7921 caggagcagg aggggcagga ggggcaggag caggaggggc aggaggggca ggagcaggag
     7981 gaggggcagg agcaggaggg gcaggagcag gaggtggagg ccggggtcga ggaggcagtg
     8041 gaggccgggg tcgaggaggt agtggaggcc ggggtcgagg aggtagtgga ggccgccggg
     8101 gtagaggacg tgaaagagcc agggggggaa gtcgtgaaag agccaggggg agaggtcgtg
     8161 gacgtggaga aaagaggccc aggagtccca gtagtcagtc atcatcatcc gggtctccac
     8221 cgcgcaggcc ccctccaggt agaaggccat ttttccaccc tgtaggggaa gccgattatt
     8281 ttgaatacca ccaagaaggt ggcccagatg gtgagcctga cgtgcccccg ggagcgatag
     8341 agcagggccc cgcagatgac ccaggagaag gcccaagcac tggaccccgg ggtcagggtg
     8401 atggaggcag gcgcaaaaaa ggagggtggt ttggaaagca tcgtggtcaa ggaggttcca
     8461 acccgaaatt tgagaacatt gcagaaggtt taagagctct cctggctagg agtcacgtag
     8521 aaaggactac cgacgaagga acttgggtcg ccggtgtgtt cgtatatgga ggtagtaaga
     8581 cctcccttta caacctaagg cgaggaactg cccttgctat tccacaatgt cgtcttacac
     8641 cattgagtcg tctccccttt ggaatggccc ctggacccgg cccacaacct ggcccgctaa
     8701 gggagtccat tgtctgttat ttcatggtct ttttacaaac tcatatattt gctgaggttt
     8761 tgaaggatgc gattaaggac cttgttatga caaagcccgc tcctacctgc aatatcaggg
     8821 tgactgtgtg cagctttgac gatggagtag atttgcctcc ctggtttcca cctatggtgg
     8881 aaggggctgc cgcggagggt gatgacggag atgacggaga tgaaggaggt gatggagatg
     8941 agggtgagga agggcaggag tgatgtaact tgttaggaga cgccctcaat cgtattaaaa
     9001 gccgtgtatt cccccgcact aaagaataaa tccccagtag acatcatgcg tgctgttggt
     9061 gtatttctgg ccatctgtct tgtcaccatt ttcgtcctcc caacatgggg caattgggca
     9121 tacccatgtt gtcacgtcac tcagctccgc gctcaacacc ttctcgcgtt ggaaaacatt
     9181 agcgacattt acctggtgag caatcagaca tgcgacggct ttagcctggc ctccttaaat
     9241 tcacctaaga atgggagcaa ccagcaggaa aaggacaagc agcgaaaatt cacgccccct
     9301 tgggaggtgg cggcatatgc aaaggatagc actcccactc tactactggg tatcatatgc
     9361 tgactgtata tgcatgagga tagcatatgc tacccggata cagattagga tagcatatac
     9421 tacccagata tagattagga tagcatatgc tacccagata tagattagga tagcctatgc
     9481 tacccagata taaattagga tagcatatac tacccagata tagattagga tagcatatgc
     9541 tacccagata tagattagga tagcctatgc tacccagata tagattagga tagcatatgc
     9601 tacccagata tagattagga tagcatatgc tatccagata tttgggtagt atatgctacc
     9661 cagatataaa ttaggatagc atatactacc ctaatctcta ttaggatagc atatgctacc
     9721 cggatacaga ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc
     9781 cagatataga ttaggatagc ctatgctacc cagatataaa ttaggatagc atatactacc
     9841 cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc
     9901 cagatataga ttaggatagc atatgctatc cagatatttg ggtagtatat gctacccatg
     9961 gcaacattag cccaccgtgc tctcagcgac ctcgtgaata tgaggaccaa caaccctgtg
    10021 cttggcgctc aggcgcaagt gtgtgtaatt tgtcctccag atcgcagcaa tcgcgcccct
    10081 atcttggccc gcccacctac ttatgcaggt attccccggg gtgccattag tggttttgtg
    10141 ggcaagtggt ttgaccgcag tggttagcgg ggttacaatc agccaagtta ttacaccctt
    10201 attttacagt ccaaaaccgc agggcggcgt gtgggggctg acgcgtgccc ccactccaca
    10261 atttcaaaaa aaagagtggc cacttgtctt tgtttatggg ccccattggc gtggagcccc
    10321 gtttaatttt cgggggtgtt agagacaacc agtggagtcc gctgctgtcg gcgtccactc
    10381 tctttcccct tgttacaaat agagtgtaac aacatggttc acctgtcttg gtccctgcct
    10441 gggacacatc ttaataaccc cagtatcata ttgcactagg attatgtgtt gcccatagcc
    10501 ataaattcgt gtgagatgga catccagtct ttacggcttg tccccacccc atggatttct
    10561 attgttaaag atattcagaa tgtttcattc ctacactagt atttattgcc caaggggttt
    10621 gtgagggtta tattggtgtc atagcacaat gccaccactg aaccccccgt ccaaatttta
    10681 ttctgggggc gtcacctgaa accttgtttt cgagcacctc acatacacct tactgttcac
    10741 aactcagcag ttattctatt agctaaacga aggagaatga agaagcaggc gaagattcag
    10801 gagagttcac tgcccgctcc ttgatcttca gccactgccc ttgtgactaa aatggttcac
    10861 taccctcgtg gaatcctgac cccatgtaaa taaaaccgtg acagctcatg gggtgggaga
    10921 tatcgctgtt ccttaggacc cttttactaa ccctaattcg atagcatatg cttcccgttg
    10981 ggtaacatat gctattgaat tagggttagt ctggatagta tatactacta cccgggaagc
    11041 atatgctacc cgtttagggt taacaagggg gccttataaa cactattgct aatgccctct
    11101 tgagggtccg cttatcggta gctacacagg cccctctgat tgacgttggt gtagcctccc
    11161 gtagtcttcc tgggcccctg ggaggtacat gtcccccagc attggtgtaa gagcttcagc
    11221 caagagttac acataaaggc aatgttgtgt tgcagtccac agactgcaaa gtctgctcca
    11281 ggatgaaagc cactcagtgt tggcaaatgt gcacatccat ttataaggat gtcaactaca
    11341 gtcagagaac ccctttgtgt ttggtccccc cccgtgtcac atgtggaaca gggcccagtt
    11401 ggcaagttgt accaaccaac tgaagggatt acatgcactg ccccgcgaag aaggggcaga
    11461 gatgtcgtag tcaggtttag ttcgtccggg gcggggatct ggccgcaaat caatctaaag
    11521 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    11581 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    11641 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    11701 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    11761 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    11821 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    11881 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    11941 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    12001 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    12061 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    12121 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    12181 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    12241 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    12301 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    12361 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    12421 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    12481 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccaccagc
    12541 tttgctctta ggagtttcct aatacatccc aaactcaaat atataaagca tttgacttgt
    12601 tctatgccct agggggcggg gggaagctaa gccagctttt tttaacattt aaaatgttaa
    12661 ttccatttta aatgcacaga tgtttttatt tcataagggt ttcaatgtgc atgaatgctg
    12721 caatattcct gttaccaaag ctagtataaa taaaaataga taaacgtgga aattacttag
    12781 agtttctgtc attaacgttt ccttcctcag ttgacaacat aaatgcgctg ctgagaagcc
    12841 agtttgcatc tgtcaggatc aatttcccat tatgccagtc atattaatta ctagtcaatt
    12901 agttgatttt tatttttgac atatacatg
//
