LOCUS       sequence-374849-       10431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The construct was used to express GFP tagged human ALDH1A1 in 
            mammalian cells for the analysis of the subcellular location of this
            protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Li J, Garavaglia S, Ye Z, Moretti A, Belyaeva OV, Beiser A, Ibrahim 
            M, Wilk A, McClellan S, Klyuyeva AV, Goggans KR, Kedishvili NY, 
            Salter EA, Wierzbicki A, Migaud ME, Mullett SJ, Yates NA, Camacho 
            CJ, Rizzi M, Sobol RW
  TITLE     A specific inhibitor of ALDH1A3 regulates retinoic acid biosynthesis
            in glioma stem cells.
  JOURNAL   Commun Biol. 2021 Dec 21;4(1):1420. doi: 10.1038/s42003-021-02949-7.
  PUBMED    34934174
REFERENCE   2  (bases 1 to 10431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-12-21"; volume: "4(1):1420. doi"; pages: " 
            10.1038/s42003-021-02949-7"
COMMENT     Sequence Label: pLenti-PGK-EGFP-ALDH1A1-Neo
FEATURES             Location/Qualifiers
     source          1..10431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(111..130)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      924..1512
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1413..1432
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1666..1683
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1800..1821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1836..1841,1842..1859,1860..1866)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1874..1890
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1879..1901
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1898..1914
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1898..1914
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1933..1953
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1935..1953
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1981..2207
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2208..2388
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2435..2560
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3053..3286
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3471..3515
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3782..3899
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3945..4455
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4374..4392
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    4510..4534
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4546..4555
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4552..4554,4555..4557,4558..5268)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4552..4554,4555..4557,4558..5268)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4597..4618)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4858..4877)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5205..5226
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6837..6861)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6892..7480
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6945..6965)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7363..7374)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7523..8022
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7558..7581)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7955..7974
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8036..8839
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8099..8118)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8709..8728
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             8930..9163
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9235..9356
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9272..9291)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9326..9345
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9396..9531
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9551..9570)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9552..9570)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9580..9597)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9580..9596)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9589..9611)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9738..10193
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9825..9844)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10035..10056
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10219..10323
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10324..10392,10393..10431,1..753)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
       61 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
      121 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
      181 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
      241 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
      301 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
      361 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
      421 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
      481 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
      541 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
      601 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
      661 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
      721 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
      781 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
      841 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
      901 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      961 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1021 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1081 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1141 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1201 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1261 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1321 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1381 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1441 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1501 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1561 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1621 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     1681 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     1741 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     1801 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     1861 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     1921 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     1981 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     2041 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     2101 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     2161 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     2221 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2281 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2341 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2401 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     2461 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     2521 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     2581 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     2641 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     2701 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     2761 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     2821 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     2881 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     2941 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     3001 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     3061 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     3121 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     3181 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     3241 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     3301 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     3361 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     3421 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     3481 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     3541 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     3601 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     3661 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     3721 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     3781 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     3841 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta
     3901 tcgatcacga gactagcctc gagaagcttg atatcgaatt ccacggggtt ggggttgcgc
     3961 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
     4021 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
     4081 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
     4141 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
     4201 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
     4261 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
     4321 gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc
     4381 gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc
     4441 gacctctctc cccaggggga tccaccggtt cgtcgactag tccagtgtgg tggaattctg
     4501 cagatatcaa caagtttgta caaaaaagca ggctcgctac cggtcgccac catggtgagc
     4561 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     4621 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     4681 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     4741 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     4801 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     4861 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     4921 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     4981 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     5041 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     5101 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     5161 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     5221 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc cggactcaga
     5281 tctcgagctc aagcttcgaa ttctgcagtc gacggtacca tgtcatcctc aggcacgcca
     5341 gacttacctg tcctactcac cgatttgaag attcaatata ctaagatctt cataaacaat
     5401 gaatggcatg attcagtgag tggcaagaaa tttcctgtct ttaatcctgc aactgaggag
     5461 gagctctgcc aggtagaaga aggagataag gaggatgttg acaaggcagt gaaggccgca
     5521 agacaggctt ttcagattgg atccccgtgg cgtactatgg atgcttccga gagggggcga
     5581 ctattataca agttggctga tttaatcgaa agagatcgtc tgctgctggc gacaatggag
     5641 tcaatgaatg gtggaaaact ctattccaat gcatatctga atgatttagc aggctgcatc
     5701 aaaacattgc gctactgtgc aggttgggct gacaagatcc agggccgtac aataccaatt
     5761 gatggaaatt tttttacata tacaagacat gaacctattg gtgtatgtgg ccaaatcatt
     5821 ccttggaatt tcccgttggt tatgctcatt tggaagatag ggcctgcact gagctgtgga
     5881 aacacagtgg ttgtcaaacc agcagagcaa actcctctca ctgctctcca cgtggcatct
     5941 ttaataaaag aggcagggtt tcctcctgga gtagtgaata ttgttcctgg ttatgggcct
     6001 acagcagggg cagccatttc ttctcacatg gatatagaca aagtagcctt cacaggatca
     6061 acagaggttg gcaagttgat caaagaagct gccgggaaaa gcaatctgaa gagggtgacc
     6121 ctggagcttg gaggaaagag cccttgcatt gtgttagctg atgccgactt ggacaatgct
     6181 gttgaatttg cacaccatgg ggtattctac caccagggcc agtgttgtat agccgcatcc
     6241 aggatttttg tggaagaatc aatttatgat gagtttgttc gaaggagtgt tgagcgggct
     6301 aagaagtata tccttggaaa tcctctgacc ccaggagtca ctcaaggccc tcagattgac
     6361 aaggaacaat atgataaaat actcgacctc attgagagtg ggaagaaaga aggggccaaa
     6421 ctggaatgtg gaggaggccc gtgggggaat aaaggctact ttgtccagcc cacagtgttc
     6481 tctaatgtta cagatgagat gcgcattgcc aaagaggaga tttttggacc agtgcagcaa
     6541 atcatgaagt ttaaatcttt agatgacgtg atcaaaagag caaacaatac tttctatggc
     6601 ttatcagcag gagtgtttac caaagacatt gataaagcca taacaatctc ctctgctctg
     6661 caggcaggaa cagtgtgggt gaattgctat ggcgtggtaa gtgcccagtg cccctttggt
     6721 ggattcaaga tgtctggaaa tggaagagaa ctgggagagt acggtttcca tgaatataca
     6781 gaggtcaaaa cagtcacagt gaaaatctct cagaagaact catctagata actgataccc
     6841 agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga caatcaacct
     6901 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6961 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     7021 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     7081 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     7141 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     7201 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     7261 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     7321 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7381 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7441 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattct gcagatatcc
     7501 ggactagtga tctaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     7561 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     7621 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     7681 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     7741 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     7801 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     7861 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
     7921 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     7981 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag ccaatatggg
     8041 atcggccatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     8101 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     8161 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     8221 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     8281 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     8341 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     8401 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     8461 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     8521 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     8581 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     8641 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     8701 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     8761 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     8821 tcttgacgag ttcttctgag gggatccgtc gagggcccgg tacctttaag accaatgact
     8881 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     8941 gctcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     9001 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     9061 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     9121 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     9181 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     9241 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     9301 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     9361 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     9421 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     9481 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     9541 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     9601 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     9661 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     9721 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     9781 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     9841 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     9901 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     9961 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
    10021 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
    10081 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
    10141 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
    10201 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
    10261 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
    10321 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
    10381 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga t
//
