LOCUS       sequence-374850-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The construct was used for expression of gRNA for knocking out of 
            the ALDH1A3 gene in Cas9 expressing cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Li J, Garavaglia S, Ye Z, Moretti A, Belyaeva OV, Beiser A, Ibrahim 
            M, Wilk A, McClellan S, Klyuyeva AV, Goggans KR, Kedishvili NY, 
            Salter EA, Wierzbicki A, Migaud ME, Mullett SJ, Yates NA, Camacho 
            CJ, Rizzi M, Sobol RW
  TITLE     A specific inhibitor of ALDH1A3 regulates retinoic acid biosynthesis
            in glioma stem cells.
  JOURNAL   Commun Biol. 2021 Dec 21;4(1):1420. doi: 10.1038/s42003-021-02949-7.
  PUBMED    34934174
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-12-21"; volume: "4(1):1420. doi"; pages: " 
            10.1038/s42003-021-02949-7"
COMMENT     Sequence Label: pLenti-ALDH1A3gRNA3
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(98..117)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      911..1499
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1400..1419
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1653..1670
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1787..1808
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1823..1828,1829..1846,1847..1853)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1861..1877
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1866..1888
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1885..1901
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1885..1901
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1920..1940
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1922..1940
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1968..2194
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2195..2375
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2422..2547
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3040..3273
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3458..3502
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        3680..3928
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3680..3700
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3851..3870
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        3950..4025
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4081..4198
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4337..5515
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4568..5506
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5463..5483
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5528..6127
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5528..5547)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6024..6044
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6143..6731
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6196..6216)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6614..6625)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6803..7036
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7114..7235
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7151..7170)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7205..7224
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7275..7410
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7430..7449)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7431..7449)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7459..7476)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7459..7475)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7468..7490)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7617..8072
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7704..7723)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7914..7935
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8098..8202
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8203..8271,8272..8323,1..740)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
       61 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
      121 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
      181 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
      241 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
      301 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
      361 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
      421 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
      481 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
      541 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
      601 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
      661 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
      721 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
      781 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
      841 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
      901 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      961 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1021 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1081 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1141 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1201 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1261 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1321 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1381 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1441 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1501 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     1561 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     1621 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     1681 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     1741 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     1801 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     1861 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     1921 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     1981 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     2041 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     2101 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     2161 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     2221 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     2281 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     2341 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     2401 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     2461 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     2521 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     2581 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     2641 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     2701 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     2761 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     2821 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     2881 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     2941 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     3001 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     3061 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     3121 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     3181 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     3241 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     3301 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     3361 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     3421 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     3481 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     3541 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     3601 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     3661 aaccccgagg ggacccagag agggcctatt tcccatgatt ccttcatatt tgcatatacg
     3721 atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa agatattagt
     3781 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
     3841 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
     3901 atatatcttg tggaaaggac gaaacaccgt gaacttgacc tccaggttgg ttttagagct
     3961 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     4021 ggtgcttttt taagcttggc gtaactagat cttgagacaa atggcagtat tcatccacaa
     4081 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     4141 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     4201 ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggggg cccgggtgca
     4261 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
     4321 gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     4381 ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg
     4441 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     4501 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     4561 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
     4621 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg
     4681 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
     4741 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
     4801 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
     4861 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
     4921 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
     4981 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
     5041 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
     5101 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
     5161 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
     5221 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
     5281 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga
     5341 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
     5401 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
     5461 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgacgtac
     5521 ggccaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     5581 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     5641 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     5701 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg tctggaccac
     5761 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     5821 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     5881 caaggagccc gcgtggttcc tggccaccgt cggagtctcg cccgaccacc agggcaaggg
     5941 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     6001 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     6061 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     6121 tgcctgaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     6181 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6241 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6301 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6361 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6421 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     6481 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     6541 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     6601 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     6661 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     6721 cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     6781 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     6841 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6901 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6961 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7021 tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa
     7081 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     7141 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7201 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     7261 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7321 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     7381 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     7441 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     7501 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     7561 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     7621 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7681 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7741 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     7801 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     7861 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     7921 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     7981 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     8041 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     8101 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8161 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     8221 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     8281 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cac
//
