LOCUS       sequence-374852-       10497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The construct was used to express GFP tagged human ALDH1A2 in 
            mammalian cells for the analysis of the subcellular location of this
            protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Li J, Garavaglia S, Ye Z, Moretti A, Belyaeva OV, Beiser A, Ibrahim 
            M, Wilk A, McClellan S, Klyuyeva AV, Goggans KR, Kedishvili NY, 
            Salter EA, Wierzbicki A, Migaud ME, Mullett SJ, Yates NA, Camacho 
            CJ, Rizzi M, Sobol RW
  TITLE     A specific inhibitor of ALDH1A3 regulates retinoic acid biosynthesis
            in glioma stem cells.
  JOURNAL   Commun Biol. 2021 Dec 21;4(1):1420. doi: 10.1038/s42003-021-02949-7.
  PUBMED    34934174
REFERENCE   2  (bases 1 to 10497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-12-21"; volume: "4(1):1420. doi"; pages: " 
            10.1038/s42003-021-02949-7"
COMMENT     Sequence Label: pLenti-PGK-EGFP-ALDH1A2-Neo
FEATURES             Location/Qualifiers
     source          1..10497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(106..174,175..966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(324..343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1137..1725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1626..1645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1879..1896
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2013..2034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2049..2054,2055..2072,2073..2079)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2087..2103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2092..2114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2111..2127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2111..2127
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2146..2166
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2148..2166
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2194..2420
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2421..2601
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2648..2773
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3266..3499
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3684..3728
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3995..4112
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4158..4668
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4587..4605
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    4723..4747
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4759..4768
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4765..4767,4768..4770,4771..5481)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4765..4767,4768..4770,4771..5481)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4810..4831)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5071..5090)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5418..5439
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(7116..7140)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7171..7759
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7224..7244)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7642..7653)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7802..8301
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7837..7860)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8234..8253
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8315..9118
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8378..8397)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8988..9007
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             9209..9442
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9514..9635
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9551..9570)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9605..9624
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9675..9810
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9830..9849)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9831..9849)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9859..9876)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9859..9875)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9868..9890)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10017..10472
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10104..10123)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10314..10335
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       61 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1801 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1861 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     1921 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     1981 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     2041 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     2101 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     2161 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     2221 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     2281 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     2341 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     2401 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     2461 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     2521 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     2581 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     2641 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     2701 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     2761 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     2821 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     2881 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     2941 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     3001 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     3061 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     3121 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     3181 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     3241 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     3301 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     3361 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     3421 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     3481 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     3541 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     3601 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     3661 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     3721 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     3781 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     3841 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     3901 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     3961 gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg
     4021 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     4081 tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgagaagc
     4141 ttgatatcga attccacggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg
     4201 cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct
     4261 cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc
     4321 cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg
     4381 tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc
     4441 cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca
     4501 gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta
     4561 gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg
     4621 tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccaggg ggatccaccg
     4681 gttcgtcgac tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa
     4741 gcaggctcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     4801 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     4861 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     4921 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     4981 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     5041 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     5101 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     5161 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     5221 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     5281 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     5341 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     5401 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     5461 ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc gaattctgca
     5521 gtcgacggta ccatgacttc cagcaagata gagatgcccg gcgaggtgaa ggccgacccc
     5581 gccgccctca tggcgtcgct gcacctcctg ccgtcgccca cgcccaatct cgaaattaag
     5641 tacaccaaga tctttataaa caacgagtgg cagaactcag agagtgggag agtgttccct
     5701 gtctataatc cagccacagg agaacaggtg tgtgaagttc aagaagcaga caaggcagat
     5761 atagacaaag cagtgcaggc agcccgcctg gctttctctc ttggttcagt gtggagaagg
     5821 atggatgctt cagaaagggg acgtctgttg gataagcttg cagacttggt ggaacgggac
     5881 agggcagttc ttgcaaccat ggaatcccta aatggtggca aaccattcct gcaagctttt
     5941 tatgtggatt tgcagggcgt catcaaaacc tttcgatatt acgcaggctg ggctgataaa
     6001 attcatggga tgaccattcc tgtagatgga gactatttta cctttacaag acatgaaccc
     6061 attggagtgt gtggacagat catcccatgg aacttccccc tgctgatgtt tgcctggaaa
     6121 atagctccag ctttgtgctg tggcaataca gtagttatta agccagcaga gcaaacacca
     6181 ctcagtgcac tctacatggg agccctcatc aaggaggctg gctttcctcc cggggtcatc
     6241 aatattttgc caggatatgg gccaacggct ggggcagcaa tagcttctca cattggcata
     6301 gacaagattg cattcacagg gtctactgag gttggaaagc ttatccaaga agcagctgga
     6361 agaagtaatt tgaagagagt aactctggaa cttggaggca aaagtcctaa tattattttt
     6421 gctgatgctg acttggacta tgctgtggag caggcccacc agggtgtgtt cttcaatcaa
     6481 ggtcagtgct gcactgcagg ctctcgcatc ttcgtggagg agtccatcta tgaggagttt
     6541 gtgagaagaa gcgtggagcg ggccaagagg cgcgtagtgg ggagtccctt tgaccccacc
     6601 actgagcagg gtccccagat tgataagaaa cagtacaaca agatcttgga actcatccag
     6661 agtggtgtgg ctgagggcgc caagctggaa tgtggaggca aaggactggg ccgaaagggg
     6721 tttttcattg agcccacagt gttttccaac gtcactgatg atatgcggat tgccaaggag
     6781 gagatctttg gccctgttca ggaaattttg agatttaaga cgatggatga agttatcgaa
     6841 agagccaata actcagactt tggactcgta gcagctgtct ttactaatga catcaacaag
     6901 gccctcacag tgtcttctgc aatgcaagct gggactgttt ggatcaattg ttacaatgcc
     6961 ttaaatgccc agagcccctt tgggggattc aagatgtctg gaaatgggag agaaatggga
     7021 gaatttggct tgcgggagta ctcagaagtt aagacggtga cagtaaagat cccccagaag
     7081 aactcctaag gatccaccgg atctagataa ctgataccca gctttcttgt acaaagtggt
     7141 tgatatccag cacagtggcg gccgctcgac aatcaacctc tggattacaa aatttgtgaa
     7201 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7261 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7321 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7381 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7441 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7501 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7561 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7621 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7681 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     7741 ctttgggccg cctccccgcc tggaattctg cagatatccg gactagtgat ctaattctac
     7801 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     7861 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     7921 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     7981 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     8041 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     8101 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     8161 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     8221 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     8281 tcctcatctc cgggcctttc gacctgcagc caatatggga tcggccattg aacaagatgg
     8341 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     8401 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     8461 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     8521 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     8581 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     8641 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     8701 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     8761 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     8821 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     8881 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     8941 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     9001 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     9061 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagg
     9121 ggatccgtcg agggcccggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     9181 agccactttt taaaagaaaa ggggggactg gaagggctag ctcactccca acgaagacaa
     9241 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     9301 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     9361 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     9421 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     9481 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     9541 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     9601 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     9661 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     9721 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     9781 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     9841 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     9901 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     9961 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
    10021 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    10081 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    10141 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
    10201 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
    10261 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
    10321 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
    10381 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
    10441 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtg
//
