LOCUS       sequence-374855-        9127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9127)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 9127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag ADRBK2
FEATURES             Location/Qualifiers
     source          1..9127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    805..1004
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1205..1621
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1551..1573
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1589..1605
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1639..1648
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1645..1686
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1711..1734
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1741..1765
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2927..2951)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3010..3471
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3066..3083)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3293..3312
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3476..4270
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3530..3549)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4140..4159
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             4457..4926
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        4962..5066
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5067..5135,5136..5927)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5285..5304)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7693..8281
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8182..8201
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8435..8452
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8556..8983)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8666..8687)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8878..8897
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgcg
      601 ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag
      661 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac
      721 ccgtcagcgg gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg
      781 accaccgacc caccaccggg aggcaagctg gccagcaact tatctgtgtc tgtccgattg
      841 tctagtgtct atgactgatt ttatgcgcct gcgtcggtac tagttagcta actagctctg
      901 tatctggcgg acccgtggtg gaactgacga gttctgaaca cccggccgca accctgggag
      961 acgtcccagg gactttgggg gccgtttttg tggcccgacc tgaggaaggg agtcgatgtg
     1021 gaatccgacc ccgtcaggat atgtggttct ggtaggagac gagaacctaa aacagttccc
     1081 gcctccgtct gaatttttgc tttcggtttg gaaccgaagc cgcgcgtctt gtctgctgca
     1141 gcgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa
     1201 ttagggccag actgttacca ctcccttaag tttgacctta ggtcactgga aagatgtcga
     1261 gcggatcgct cacaaccagt cggtagatgt caagaagaga cgttgggtta ccttctgctc
     1321 tgcagaatgg ccaaccttta acgtcggatg gccgcgagac ggcaccttta accgagacct
     1381 catcacccag gttaagatca aggtcttttc acctggcccg catggacacc cagaccaggt
     1441 cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg tcaagccctt
     1501 tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc
     1561 tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt ctctaggcgc
     1621 cggccggatc cagaattcgc caccatgggg tcttcaaaat ctaaaccaaa ggaccccagc
     1681 cagcgccggc gcaggatccg aggttacctt gactacaaag acgatgacga caagcaattg
     1741 acaagtttgt acaaaaaagt tggcatggcg gacctggagg ctgtgctggc cgatgtcagt
     1801 tacctgatgg ccatggagaa gagcaaggcg accccggccg cccgcgccag caagaggatc
     1861 gtcctgccgg agcccagtat ccggagtgtg atgcagaagt accttgcaga gagaaatgaa
     1921 ataacctttg acaagatttt caatcagaaa attggtttct tgctatttaa agatttttgt
     1981 ttgaatgaaa ttaatgaagc tgtacctcag gtgaagtttt atgaagagat aaaggaatat
     2041 gaaaaacttg ataatgagga agaccgcctt tgcagaagtc gacaaattta tgatgcctac
     2101 atcatgaagg aacttctttc ctgttcacat cctttctcaa agcaagctgt agaacacgta
     2161 caaagtcatt tatccaagaa acaagtgaca tcaactcttt ttcagccata catagaagaa
     2221 atttgtgaaa gccttcgagg tgacattttt caaaaattta tggaaagtga caagttcact
     2281 agattttgtc agtggaaaaa cgttgaatta aatatccatt tgaccatgaa tgagttcagt
     2341 gtgcatagga ttattggacg aggaggattc ggggaagttt atggttgcag gaaagcagac
     2401 actggaaaaa tgtatgcaat gaaatgctta gataagaaga ggatcaaaat gaaacaagga
     2461 gaaacattag ccttaaatga aagaatcatg ttgtctcttg tcagcacagg agactgtcct
     2521 ttcattgtat gtatgaccta tgccttccat accccagata aactctgctt catcctggat
     2581 ctgatgaacg ggggcgattt gcactaccac ctttcacaac acggtgtgtt ctctgagaag
     2641 gagatgcggt tttatgccac tgaaatcatt ctgggtctgg aacacatgca caatcggttt
     2701 gttgtctaca gagatttgaa gccagcaaat attctcttgg atgaacatgg acacgcaaga
     2761 atatcagatc ttggtcttgc ctgcgatttt tccaaaaaga agcctcatgc gagtgttggc
     2821 acccatgggt acatggctcc cgaggtgctg cagaagggga cggcctatga cagcagtgcc
     2881 gactggttct ccctgggctg catgcttttc aaacttctga gaggatgccc aactttcttg
     2941 tacaaagtgg tttgatctag tcgaccaatt ccggttattt tccaccatat tgccgtcttt
     3001 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     3061 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     3121 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     3181 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     3241 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     3301 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     3361 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaacgtcta
     3421 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata ataccatgat
     3481 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     3541 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     3601 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     3661 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     3721 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     3781 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     3841 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     3901 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     3961 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     4021 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     4081 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     4141 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     4201 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     4261 gttcttctga gcgggactct ggggttcgca tcgataaaat aaaagatttt atttagtctc
     4321 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg
     4381 ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca
     4441 ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     4501 cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
     4561 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc
     4621 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
     4681 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
     4741 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt
     4801 gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg
     4861 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt
     4921 ctttcacagg ccagtggggc cgcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4981 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5041 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5101 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5161 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5221 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5281 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5341 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5401 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5461 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5521 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5581 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5641 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5701 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5761 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5821 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5881 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5941 tttactcata tatactttag attgatttgc ggccgcgaaa aggacaagca gcgaaaattc
     6001 acgccccctt gggaggtggc ggcatatgca aaggatagca ctcccactct actactgggt
     6061 atcatatgct gactgtatat gcatgaggat agcatatgct acccggatac agattaggat
     6121 agcatatact acccagatat agattaggat agcatatgct acccagatat agattaggat
     6181 agcctatgct acccagatat aaattaggat agcatatact acccagatat agattaggat
     6241 agcatatgct acccagatat agattaggat agcctatgct acccagatat agattaggat
     6301 agcatatgct atccagatat ttgggtagta tatgctaccc atggcaacat tagcccaccg
     6361 tgctctcagc gacctcgtga atatgaggac caacaaccct gtgcttggcg ctcaggcgca
     6421 agtgtgtgta atttgtcctc cagatcgcag caatcgcgcc cctatcttgg cccgcccacc
     6481 tacttatgca ggtattcccc ggggtgccat tagtggtttt gtgggcaagt ggtttgaccg
     6541 cagtggttag cggggttaca atcagccaag ttattacacc cttattttac agtccaaaac
     6601 cgcagggcgg cgtgtggggg ctgacgcgtg cccccactcc acaatttcaa aaaaaagagt
     6661 ggccacttgt ctttgtttat gggccccatt ggcgtggagc cccgtttaat tttcgggggt
     6721 gttagagaca accagtggag tccgctgctg tcggcgtcca ctctctttcc ccttgttaca
     6781 aatagagtgt aacaacatgg ttcacctgtc ttggtccctg cctgggacac atcttaataa
     6841 ccccagtatc atattgcact aggattatgt gttgcccata gccataaatt cgtgtgagat
     6901 ggacatccag tctttacggc ttgtccccac cccatggatt tctattgtta aagatattca
     6961 gaatgtttca ttcctacact agtatttatt gcccaagggg tttgtgaggg ttatattggt
     7021 gtcatagcac aatgccacca ctgaaccccc cgtccaaatt ttattctggg ggcgtcacct
     7081 gaaaccttgt tttcgagcac ctcacataca ccttactgtt cacaactcag cagttattct
     7141 attagctaaa cgaaggagaa tgaagaagca ggcgaagatt caggagagtt cactgcccgc
     7201 tccttgatct tcagccactg cccttgtgac taaaatggtt cactaccctc gtggaatcct
     7261 gaccccatgt aaataaaacc gtgacagctc atggggtggg agatatcgct gttccttagg
     7321 acccttttac taaccctaat tcgatagcat atgcttcccg ttgggtaaca tatgctattg
     7381 aattagggtt agtctggata gtatatacta ctacccggga agcatatgct acccgtttag
     7441 ggttaacaag ggggccttat aaacactatt gctaatgccc tcttgagggt ccgcttatcg
     7501 gtagctacac aggcccctct gattgacgtt ggtgtagcct cccgtagtct tcctgggccc
     7561 ctgggaggta catgtcggcc gcaaaaggat ctaggtgaag atcctttttg ataatctcat
     7621 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7681 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7741 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7801 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7861 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7921 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7981 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8041 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8101 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8161 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8221 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8281 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8341 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8401 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8461 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcaacta
     8521 tggttggcgc gttgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     8581 cagctcattt tttaaccaat agaccgaaat cggcaaaatc ccttataaat caaaagaata
     8641 gcccgagata gagttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     8701 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     8761 atcacccaaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     8821 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     8881 gaagaaagcg aaaggagcgg gcgctaaggc gctggcaagt gtagcggtca cgctgcgcgt
     8941 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgccgatct tcaatattgg
     9001 ccattagcca tattattcat tggttatata gcataaatca atattggcta ttggccattg
     9061 catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg
     9121 ccatgtt
//
