LOCUS       sequence-374860-        8878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sgRNA targeting enhancer 1 of MYC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8878)
  AUTHORS   Lin X, Liu Y, Liu S, Zhu X, Wu L, Zhu Y, Zhao D, Xu X, Chemparathy 
            A, Wang H, Cao Y, Nakamura M, Noordermeer JN, La Russa M, Wong WH, 
            Zhao K, Qi LS
  TITLE     Nested epistasis enhancer networks for robust genome regulation.
  JOURNAL   Science. 2022 Aug 11:eabk3512. doi: 10.1126/science.abk3512.
  PUBMED    35951677
REFERENCE   2  (bases 1 to 8878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2022 Aug 11:eabk3512. doi: 10.1126/science.abk3512."
COMMENT     Sequence Label: pSLQ1373_mU6_sgRNA targeting e1
FEATURES             Location/Qualifiers
     source          1..8878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2..18)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             527..707
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(769..1357)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(849..868)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1528..2319,2320..2388))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2151..2170
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2389..2493)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2568..2587)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2759..3138
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3140..3338
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3293..3313
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3356..3536
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3583..3708
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4205..4438
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4623..4667
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4965..5082
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(5335..5368)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     5372..5392
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        5638..5849
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          5850..6788
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6745..6765
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6809..7405
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6809..6828)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7305..7325
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             7415..7468
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7481..8179
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     protein_bind    complement(8199..8232)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    8288..8876
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8341..8361)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8759..8770)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgataccgtc gacctcgatc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
       61 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
      121 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
      181 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
      241 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
      301 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
      361 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
      421 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
      481 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
      541 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
      601 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
      661 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca tgtgagcaaa
      721 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      781 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      841 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      901 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      961 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1021 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1081 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1141 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1201 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1261 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1321 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1381 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1441 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1501 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     1561 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     1621 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     1681 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     1741 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     1801 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     1861 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     1921 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     1981 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     2041 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     2101 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     2161 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     2221 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     2281 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     2341 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     2401 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     2461 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     2521 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     2581 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     2641 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     2701 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     2761 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     2821 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     2881 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     2941 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     3001 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3061 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3121 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     3181 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     3241 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     3301 ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc
     3361 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     3421 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     3481 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     3541 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     3601 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     3661 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     3721 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     3781 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     3841 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     3901 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     3961 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     4021 aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag
     4081 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     4141 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     4201 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     4261 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     4321 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     4381 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     4441 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     4501 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     4561 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     4621 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     4681 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     4741 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     4801 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     4861 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     4921 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     4981 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     5041 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     5101 agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc gccatctcta
     5161 ggcccgcgcc ggccccctcg cacggacttg tgggagaagc tcggctactc ccctgccccg
     5221 gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga taaaagacag
     5281 ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat ttctataact
     5341 tcgtatagca tacattatac gaagttataa acagcacaaa aggaaactca ccctaactgt
     5401 aaagtaattg tgtgttttga gactataagt atcccttgga gaaccacctt gttggaacct
     5461 tcagggaaaa ggcttgttta agagctaagc tggaaacagc atagcaagtt taaataaggc
     5521 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttct cgagtactag
     5581 gatccattag gcggccgcgt ggataaccgt attaccgcca tgcatgtgcc cgtcagtggg
     5641 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     5701 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     5761 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     5821 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     5881 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     5941 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     6001 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     6061 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     6121 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     6181 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     6241 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     6301 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     6361 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     6421 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     6481 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     6541 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgcgc ttttggagta
     6601 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     6661 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     6721 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     6781 catttcaggt gtcgtgagct agcccaccat gaccgagtac aagcccacgg tgcgcctcgc
     6841 cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     6901 cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga
     6961 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     7021 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     7081 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     7141 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     7201 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     7261 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     7321 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     7381 gtgcatgacc cgcaagcccg gtgccggatc gggagagggc agaggaagtc tgctaacatg
     7441 cggtgacgtc gaggagaatc ctggcccacc ggtcgccacc agcgagctga ttaaggagaa
     7501 catgcacatg aagctgtaca tggagggcac cgtggacaac catcacttca agtgcacatc
     7561 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg
     7621 cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg gcagcaagac
     7681 cttcatcaac cacacccagg gcatccccga cttcttcaag cagtccttcc ctgagggctt
     7741 cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
     7801 cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcacatc
     7861 caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg agacgctgta
     7921 ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag
     7981 ccatctgatc gcaaacatca agaccacata tagatccaag aaacccgcta agaacctcaa
     8041 gatgcctggc gtctactatg tggactacag actggaaaga atcaaggagg ccaacaacga
     8101 gacctacgtc gagcagcacg aggtggcagt ggccagatac tgcgacctcc ctagcaaact
     8161 ggggcacaag cttaattaag aattcgtcga gggacctaat aacttcgtat agcatacatt
     8221 atacgaagtt atacatgttt aagggttccg gttccactag gtacaattcg atatcaagct
     8281 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     8341 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     8401 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     8461 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     8521 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     8581 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     8641 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     8701 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     8761 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     8821 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcat
//
