LOCUS       sequence-374865-        5306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmids for Teal Fluorescent Genetically 
            Encoded Calcium Indicator T-GECO1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Aggarwal A, Sunil S, Bendifallah I, Moon M, Drobizhev M, Zarowny L, 
            Zheng J, Wu SY, Lohman AW, Tebo AG, Emiliani V, Podgorski K, Shen Y,
            Campbell RE
  TITLE     Blue-shifted genetically encoded Ca(2+) indicator with enhanced 
            two-photon absorption.
  JOURNAL   Neurophotonics. 2024 Apr;11(2):024207. doi: 
            10.1117/1.NPh.11.2.024207.
  PUBMED    38577628
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neurophotonics."; date: "2024-04"; volume: "11(2):024207. doi"; 
            pages: " 10.1117/1.NPh.11.2.024207"
COMMENT     Sequence Label: pBAD-T-GECO1
FEATURES             Location/Qualifiers
     source          1..5306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      39..66
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        85..176
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(177..245,246..1037)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(395..414)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1208..1796
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1697..1716
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1950..1967
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1982..2122
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2048..2067)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2208..2230
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2386..3264)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3291..3575
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3498..3517
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             3621..3638
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3642..3674
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(3678..3686,3687..3701)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             4638..4694
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RHKWNKTGHAVRAIGRLSS"
     primer_bind     complement(4997..5014)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      5167..5253
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg
       61 cctttttgcg tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc
      121 cgctcatgag acaataaccc tgataaatgc tttaataata ttgaaaaagg aagagtatga
      181 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      241 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      301 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      361 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
      421 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      481 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
      541 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
      601 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
      661 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
      721 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
      781 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
      841 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
      901 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
      961 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1021 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1081 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1141 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1201 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaacaaccac
     1261 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1321 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1381 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1441 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1501 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1561 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1621 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1681 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1741 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1801 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1861 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1921 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     1981 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     2041 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     2101 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     2161 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     2221 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagcagatca attcgcgcgc
     2281 gaaggcgaag cggcatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac
     2341 cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac
     2401 ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt
     2461 gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac
     2521 ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc
     2581 ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga
     2641 cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg
     2701 atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc
     2761 gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc
     2821 cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg
     2881 cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt
     2941 aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg
     3001 agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc
     3061 cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag
     3121 attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt
     3181 ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag
     3241 gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa
     3301 ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac
     3361 caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac
     3421 aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca
     3481 cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg
     3541 acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc taacaggagg
     3601 aattaaccat ggggggttct catcatcatc atcatcatgg tatggctagc atgactggtg
     3661 gacagcaaat gggtcgggat ctgtacgacg atgacgataa ggatccgagc tcgagaatgg
     3721 tgagcaaggg cgatgagacc acaatgggcg taatcaagcc cgacatgaag atcaagctga
     3781 agatggaggg caacgtgaat ggccacgcct tcgtgatcga gggcgagggc gagggcaagc
     3841 cctacgacgg cacccacacc atcaacctgg aggtgaagga gggagccccc ctgcccttct
     3901 cctacgacat cctgaccacc gcgttcgcct acggcaacag ggccttcacc aagtaccccg
     3961 acgacatccc cgactacttc aagcagtcct tccccgaggg ctactcttgg gagcgcatca
     4021 tgaccttcga ggacaagggc atcgtgaagg tgaagtccga catctcgatg gaggagggct
     4081 ccttcatcta cgaaatatac ctcaagggcg agaacttccc ccccaacggt cccgtgatgc
     4141 agaagaagac caccggctgg cggaatcgtg atcaactgac tgaagagcag atcgcagaat
     4201 ttaaagaggc tttctcccta tttgataagg acggggatgg aacaataaca accgaggagc
     4261 tggggacggt gatgcggtct ctggggcaga accccacaga agcagagctg caggacatga
     4321 tcaatgtagt agatgccgac ggtgacggcg caatcgactt ccctgagttc ctgacaatga
     4381 tgtcaagaaa aatgaactac agggacacgg aagaggaaat tagagaagcg ttcggtgtgt
     4441 ttgataagga tggcaatggc tacatcagtg cagcagagct tcaccgcgtg atgacaaacc
     4501 ttggagaaaa gttatcagat gaagaggttg atgatgtgat cagggaagca gacatcgatg
     4561 gggatggtct ggtaaactac gaagagtttg tacaaatgat gacagcgaag ggtggcggag
     4621 gttctgtcga ctcatcacgt cataagtgga ataagacagg tcacgcagtc agagctatag
     4681 gtcggctgag ctcaattggg ggtgagaaga tgtacgtgcg cgacggcgtg ctgaagggcg
     4741 acgtcgagca cgagctgctg ctgaagggcg gcggccacca ccgcgttgac ttcaagacca
     4801 tctacagggc caagaaggcg gtgaagctgc ccgactatca ctttgtggac caccacatcg
     4861 agatcctgaa ccacgacaag gactacaaca aggtgaccgt ctacgagagc gccgcggccc
     4921 gcaactcccg taggggcatg gacgagctgt acaagtaaaa gcttggctgt tttggcggat
     4981 gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac
     5041 agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag
     5101 tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc
     5161 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     5221 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg
     5281 aagcaacggc ccggagggtg gcgggc
//
