LOCUS       sequence-374893-        3494 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive T7 RNAP-mediated expression of LuxR transcription 
            factor for Lux quorum sensing system. LuxR sequence is codon 
            optimized for E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Gonzales DT, Suraritdechachai S, Zechner C, Tang T-YD
  TITLE     Bidirectional communication between droplet interface bilayers 
            driven by cell-free quorum sensing gene circuits
  JOURNAL   ChemSystemsChem 2023
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "ChemSystemsChem 2023"
COMMENT     Sequence Label: pEXP5-NT/pT7 LuxR
FEATURES             Location/Qualifiers
     source          1..3494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(22..40)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      36..83
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(128..146)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        214..318
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(319..387,388..1179)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(537..556)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1350..1938
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1839..1858
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2092..2109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2189..2208)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2567..2586
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2567..2585
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2617..2639
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2658..2675
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2688..2708
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
       61 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggataa ttcttgaaga
      121 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      181 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      241 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      301 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      361 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
      421 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
      481 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
      541 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
      601 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
      661 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
      721 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
      781 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
      841 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
      901 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
      961 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     1021 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     1081 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     1141 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     1201 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1261 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1321 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1381 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1441 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1501 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1561 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1621 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1681 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1741 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1801 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1861 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     1921 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     1981 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2041 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2101 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     2161 atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     2221 agtatacact ccgctatcgc tacgcttcgt taatacagat gtaggtgttc cacagggtag
     2281 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt
     2341 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga
     2401 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc
     2461 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac
     2521 ccgtggccag gacccaacgc tgcccgagat ctcgatcccg cgaaattaat acgactcact
     2581 atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat
     2641 ataccatgtc tggttctcat catcatcatc atcatggtag cagcggcgaa aacctgtatt
     2701 ttcagtccat gaaaaacatc aatgccgacg acacctaccg cattatcaat aaaatcaaag
     2761 cgtgtcgcag caataatgac atcaatcaat gcctgtctga tatgaccaaa atggtgcatt
     2821 gtgaatatta tctgctggcg attatttatc cgcattctat ggtgaaatct gatattagca
     2881 ttctggataa ttacccgaaa aaatggcgtc aatattatga tgacgcgaac cttatcaaat
     2941 atgacccgat tgtggattat tctaactcca atcatagccc aatcaattgg aatatttttg
     3001 aaaacaatgc ggtgaataaa aaatctccga atgttatcaa agaagcgaaa accagcggtc
     3061 tgattacggg ctttagtttc ccaattcata cggcgaacaa tggcttcggt atgctgagtt
     3121 ttgcacatag cgaaaaagac aactatattg atagtctgtt tctgcacgcg tgtatgaaca
     3181 ttccgctgat tgttccgtct ctggttgata attatcgcaa aatcaatatt gcaaataata
     3241 aaagcaacaa cgacctgacc aaacgcgaaa aagaatgtct ggcgtgggcg tgcgaaggca
     3301 aatcgtcttg ggatattagc aaaattctgg gttgcagtga acgcaccgtc accttccatc
     3361 tgaccaatgc ccaaatgaaa ctgaatacca ccaaccgctg ccaaagtatt tctaaagcaa
     3421 ttctgacggg tgcaattgat tgcccgtatt tcaaaaacta aagggtgatc cggctgctaa
     3481 caaagcccga aagg
//
