LOCUS       sequence-37491-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Subach FV, Patterson GH, Manley S, Gillette JM, Lippincott-Schwartz 
            J, Verkhusha VV
  TITLE     Photoactivatable mCherry for high-resolution two-color fluorescence 
            microscopy.
  JOURNAL   Nat Methods. 2009 Feb;6(2):153-9. Epub 2009 Jan 25.
  PUBMED    19169259
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2009-02"; volume: "6(2)"; pages: "153-9. Epub 2009
            Jan 25"
COMMENT     Sequence Label: pPAmCherry2-C1
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..740
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein"
                     /label=PAmCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHLEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWETLSERMYPEDGALK
                     GELKARTKLKDGGHYDTEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGLHSTGGMDELYK"
     primer_bind     complement(178..196)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     446..465
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    741..806
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gtgaaccgtc agatccgcta gcggtcgcca ccatggtgag caagggcgag gaggataaca
       61 tggccatcat caaggagttc atgcgcttca aggtgcacct ggaggggtcc gtgaacggcc
      121 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      181 agctgaaggt gaccaagggt ggccccttgc ccttcgcctg ggacatcctg tcccctcagt
      241 tcatgtacgg ctccaatgcc tacgtgaagc accccgccga catccccgac tactttaagc
      301 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaagac ggcggcgtgg
      361 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
      421 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggaga
      481 ccctctccga gcggatgtac cccgaggacg gcgccctgaa gggagagctc aaggcgagga
      541 cgaagctgaa ggacggcggc cactatgaca ctgaggtcaa gaccacctac aaggccaaga
      601 agcccgtgca gttgcccggc gcctacaacg tcaaccgcaa gttggatatc acctcccaca
      661 acgaggacta caccatcgtg gaacagtacg aacgtgccga gggcctccac tccaccggcg
      721 gcatggacga gctgtacaag tccggactca gatctcgagc tcaagcttcg aattctgcag
      781 tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata
      841 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
      901 aacataaaat g
//
