LOCUS       sequence-37498-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Peterson TR, Sengupta SS, Harris TE, Carmack AE, Kang SA, Balderas 
            E, Guertin DA, Madden KL, Carpenter AE, Finck BN, Sabatini DM
  TITLE     mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP 
            Pathway.
  JOURNAL   Cell. 2011 Aug 5;146(3):408-20.
  PUBMED    21816276
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-08-5"; volume: "146(3)"; pages: "408-20"
COMMENT     Sequence Label: pLKO-puro human lipin 1-1 shRNA
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     130..149
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    305..422
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     900..918
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 cgatcaggct gttgagagat aattggaatt aatttgactg taaacacaaa gatattagta
       61 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
      121 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
      181 tatatcttgt ggaaaggacg aaacaccggc ctcagacaga aatgcagttt ctcgagaaac
      241 tgcatttctg tctgaggttt tttgaattct cgacctcgag acaaatggca gtattcatcc
      301 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
      361 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
      421 ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg
      481 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
      541 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
      601 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
      661 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
      721 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
      781 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
      841 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
      901 tgttccgcat tctgcaagcc tccggag
//
