LOCUS       sequence-374991-       14472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP-LacI-p300 fusion plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14472)
  AUTHORS   Pfisterer M, Schmitz ML
  TITLE     Testing the Effect of Histone Acetyltransferases on Local Chromatin 
            Compaction.
  JOURNAL   Methods Mol Biol. 2023;2589:361-376. doi: 
            10.1007/978-1-0716-2788-4_24.
  PUBMED    36255637
REFERENCE   2  (bases 1 to 14472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2023"; volume: "2589:361-376. doi"; pages: " 
            10.1007/978-1-0716-2788-4_24"
COMMENT     Sequence Label: GFP-LacI-p300
FEATURES             Location/Qualifiers
     source          1..14472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             117..911
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(171..190)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     781..800
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1085..1206
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1122..1141)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1176..1195
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1255..1271)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1255..1271)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1268..1290)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1279..1295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1303..1309,1310..1327,1328..1333))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1348..1369
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1486..1503)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1657..2245)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1737..1756)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2416..3207,3208..3276))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3039..3058
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3277..3381)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3456..3475)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3647..4026
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4027..4230
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4181..4201
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4275..4294
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4275..4293
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4343..4352
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4349..4351,4352..4354,4355..5065)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4349..4351,4352..4354,4355..5065)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4394..4415)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4655..4674)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5002..5023
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5103..5122)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             9320..11170
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      10934..10943
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(13475..13492)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    13481..13705
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      13751..14179
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13838..13857)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14048..14069
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(14188..14208)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(14193..14472,1..50)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(14373..14472,1..36)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     14435..14454
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
       61 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
      121 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
      181 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
      241 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
      301 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
      361 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
      421 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
      481 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
      541 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
      601 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
      661 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
      721 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
      781 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
      841 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
      901 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
      961 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     1021 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     1081 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     1141 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     1201 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     1261 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     1321 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     1381 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     1441 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     1501 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1561 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1621 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1681 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1741 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1801 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1861 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1921 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1981 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2041 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     2101 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2161 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2221 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2281 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2341 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     2401 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     2461 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     2521 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     2581 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     2641 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     2701 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     2761 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     2821 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     2881 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     2941 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3001 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     3061 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3121 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3181 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3241 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3301 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     3361 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     3421 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     3481 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     3541 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     3601 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     3661 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     3721 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     3781 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     3841 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     3901 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     3961 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     4021 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     4081 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     4141 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     4201 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac
     4261 tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta gcgctaccgg
     4321 ggccgctcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
     4381 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     4441 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     4501 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     4561 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     4621 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     4681 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     4741 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     4801 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     4861 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     4921 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     4981 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     5041 tctcggcatg gacgagctgt acaagtccgg actcagatcc atggtgaaac cagtaacgtt
     5101 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
     5161 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa
     5221 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt
     5281 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
     5341 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
     5401 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
     5461 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
     5521 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
     5581 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
     5641 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
     5701 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
     5761 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
     5821 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
     5881 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt
     5941 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
     6001 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
     6061 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6121 aatgcagctg gcacgacagg tttcccgaag cagcctgagg gggcgtcgac tgggtaccat
     6181 ggccgagaat gtggtggaac cggggccgcc ttcagccaag cggcctaaac tctcatctcc
     6241 ggccctctcg gcgtccgcca gcgatggcac agattttggc tctctatttg acttggagca
     6301 cgacttacca gatgaattaa tcaactctac agaattggga ctaaccaatg gtggtgatat
     6361 taatcagctt cagacaagtc ttggcatggt acaagatgca gcttctaaac ataaacagct
     6421 gtcagaattg ctgcgatctg gtagttcccc taacctcaat atgggagttg gtggcccagg
     6481 tcaagtcatg gccagccagg cccaacagag cagtcctgga ttaggtttga taaatagcat
     6541 ggtcaaaagc ccaatgacac aggcaggctt gacttctccc aacatgggga tgggcactag
     6601 tggaccaaat cagggtccta cgcagtcaac aggtatgatg aacagtccag taaatcagcc
     6661 tgccatggga atgaacacag ggatgaatgc gggcatgaat cctggaatgt tggctgcagg
     6721 caatggacaa gggataatgc ctaatcaagt catgaacggt tcaattggag caggccgagg
     6781 gcgacagaat atgcagtacc caaacccagg catgggaagt gctggcaact tactgactga
     6841 gcctcttcag cagggctctc cccagatggg aggacaaaca ggattgagag gcccccagcc
     6901 tcttaagatg ggaatgatga acaaccccaa tccttatggt tcaccatata ctcagaatcc
     6961 tggacagcag attggagcca gtggccttgg tctccagatt cagacaaaaa ctgtactatc
     7021 aaataactta tctccatttg ctatggacaa aaaggcagtt cctggtggag gaatgcccaa
     7081 catgggtcaa cagccagccc cgcaggtcca gcagccaggt ctggtgactc cagttgccca
     7141 agggatgggt tctggagcac atacagctga tccagagaag cgcaagctca tccagcagca
     7201 gcttgttctc cttttgcatg ctcacaagtg ccagcgccgg gaacaggcca atggggaagt
     7261 gaggcagtgc aaccttcccc actgtcgcac aatgaagaat gtcctaaacc acatgacaca
     7321 ctgccagtca ggcaagtctt gccaagtggc acactgtgca tcttctcgac aaatcatttc
     7381 acactggaag aattgtacaa gacatgattg tcctgtgtgt ctccccctca aaaatgctgg
     7441 tgataagaga aatcaacagc caattttgac tggagcaccc gttggacttg gaaatcctag
     7501 ctctctaggg gtgggtcaac agtctgcccc caacctaagc actgttagtc agattgatcc
     7561 cagctccata gaaagagcct atgcagctct tggactaccc tatcaagtaa atcagatgcc
     7621 gacacaaccc caggtgcaag caaagaacca gcagaatcag cagcctgggc agtctcccca
     7681 aggcatgcgg cccatgagca acatgagtgc tagtcctatg ggagtaaatg gaggtgtagg
     7741 agttcaaacg ccgagtcttc tttctgactc aatgttgcat tcagccataa attctcaaaa
     7801 cccaatgatg agtgaaaatg ccagtgtgcc ctccctgggt cctatgccaa cagcagctca
     7861 accatccact actggaattc ggaaacagtg gcacgaagat attactcagg atcttcgaaa
     7921 tcatcttgtt cacaaactcg tccaagccat atttcctacg ccggatcctg ctgctttaaa
     7981 agacagacgg atggaaaacc tagttgcata tgctcggaaa gttgaagggg acatgtatga
     8041 atctgcaaac aatcgagcgg aatactacca ccttctagct gagaaaatct ataagatcca
     8101 gaaagaacta gaagaaaaac gaaggaccag actacagaag cagaacatgc taccaaatgc
     8161 tgcaggcatg gttccagttt ccatgaatcc agggcctaac atgggacagc cgcaaccagg
     8221 aatgacttct aatggccctc tacctgaccc aagtatgatc cgtggcagtg tgccaaacca
     8281 gatgatgcct cgaataactc cacaatctgg tttgaatcaa tttggccaga tgagcatggc
     8341 ccagccccct attgtacccc ggcaaacccc tcctcttcag caccatggac agttggctca
     8401 acctggagct ctcaacccgc ctatgggcta tgggcctcgt atgcaacagc cttccaacca
     8461 gggccagttc cttcctcaga ctcagttccc atcacaggga atgaatgtaa caaatatccc
     8521 tttggctccg tccagcggtc aagctccagt gtctcaagca caaatgtcta gttcttcctg
     8581 cccggtgaac tctcctataa tgcctccagg gtctcagggg agccacattc actgtcccca
     8641 gcttcctcaa ccagctcttc atcagaattc accctcgcct gtacctagtc gtacccccac
     8701 ccctcaccat actcccccaa gcataggggc tcagcagcca ccagcaacaa caattccagc
     8761 ccctgttcct acacctcctg ccatgccacc tgggccacag tcccaggctc tacatccccc
     8821 tccaaggcag acacctacac caccaacaac acaacttccc caacaagtgc agccttcact
     8881 tcctgctgca ccttctgctg accagcccca gcagcagcct cgctcacagc agagcacagc
     8941 agcgtctgtt cctaccccaa cagcaccgct gcttcctccg cagcctgcaa ctccactttc
     9001 ccagccagct gtaagcattg aaggacaggt atcaaatcct ccatctacta gtagcacaga
     9061 agtgaattct caggccattg ctgagaagca gccttcccag gaagtgaaga tggaggccaa
     9121 aatggaagtg gatcaaccag aaccagcaga tactcagccg gaggatattt cagagtctaa
     9181 agtggaagac tgtaaaatgg aatctaccga aacagaagag agaagcactg agttaaaaac
     9241 tgaaataaaa gaggaggaag accagccaag tacttcagct acccagtcat ctccggctcc
     9301 aggacagtca aagaaaaaga ttttcaaacc agaagaacta cgacaggcac tgatgccaac
     9361 tttggaggca ctttaccgtc aggatccaga atcccttccc tttcgtcaac ctgtggaccc
     9421 tcagctttta ggaatccctg attactttga tattgtgaag agccccatgg atctttctac
     9481 cattaagagg aagttagaca ctggacagta tcaggagccc tggcagtatg tcgatgatat
     9541 ttggcttatg ttcaataatg cctggttata taaccggaaa acatcacggg tatacaaata
     9601 ctgctccaag ctctctgagg tctttgaaca agaaattgac ccagtgatgc aaagccttgg
     9661 atactgttgt ggcagaaagt tggagttctc tccacagaca ctgtgttgct acggcaaaca
     9721 gttgtgcaca atacctcgtg atgccactta ttacagttac cagaacaggt atcatttctg
     9781 tgagaagtgt ttcaatgaga tccaagggga gagcgtttct ttgggggatg acccttccca
     9841 gcctcaaact acaataaata aagaacaatt ttccaagaga aaaaatgaca cactggatcc
     9901 tgaactgttt gttgaatgta cagagtgcgg aagaaagatg catcagatct gtgtccttca
     9961 ccatgagatc atctggcctg ctggattcgt ctgtgatggc tgtttaaaga aaagtgcacg
    10021 aactaggaaa gaaaataagt tttctgctaa aaggttgcca tctaccagac ttggcacctt
    10081 tctagagaat cgtgtgaatg actttctgag gcgacagaat caccctgagt caggagaggt
    10141 cactgttaga gtagttcatg cttctgacaa aaccgtggaa gtaaaaccag gcatgaaagc
    10201 aaggtttgtg gacagtggag agatggcaga atcctttcca taccgaacca aagccctctt
    10261 tgcctttgaa gaaattgatg gtgttgacct gtgcttcttt ggcatgcatg ttcaagagta
    10321 tggctctgac tgccctccac ccaaccagag gagagtatac atatcttacc tcgatagtgt
    10381 tcatttcttc cgtcctaaat gcttgaggac tgcagtctat catgaaatcc taattggata
    10441 tttagaatat gtcaagaaat taggttacac aacagggcat atttgggcat gtccaccaag
    10501 tgagggagat gattatatct tccattgcca tcctcctgac cagaagatac ccaagcccaa
    10561 gcgactgcag gaatggtaca aaaaaatgct tgacaaggct gtatcagagc gtattgtcca
    10621 tgactacaag gatattttta aacaagctac tgaagataga ttaacaagtg caaaggaatt
    10681 gccttatttc gagggtgatt tctggcccaa tgttctggaa gaaagcatta aggaactgga
    10741 acaggaggaa gaagagagaa aacgagagga aaacaccagc aatgaaagca cagatgtgac
    10801 caagggagac agcaaaaatg ctaaaaagaa gaataataag aaaaccagca aaaataagag
    10861 cagcctgagt aggggcaaca agaagaaacc cgggatgccc aatgtatcta acgacctctc
    10921 acagaaacta tatgccacca tggagaagca taaagaggtc ttctttgtga tccgcctcat
    10981 tgctggccct gctgccaact ccctgcctcc cattgttgat cctgatcctc tcatcccctg
    11041 cgatctgatg gatggtcggg atgcgtttct cacgctggca agggacaagc acctggagtt
    11101 ctcttcactc cgaagagccc agtggtccac catgtgcatg ctggtggagc tgcacacgca
    11161 gagccaggac cgctttgtct acacctgcaa tgaatgcaag caccatgtgg agacacgctg
    11221 gcactgtact gtctgtgagg attatgactt gtgtatcacc tgctataaca ctaaaaacca
    11281 tgaccacaaa atggagaaac taggccttgg cttagatgat gagagcaaca accagcaggc
    11341 tgcagccacc cagagcccag gcgattctcg ccgcctgagt atccagcgct gcatccagtc
    11401 tctggtccat gcttgccagt gtcggaatgc caattgctca ctgccatcct gccagaagat
    11461 gaagcgggtt gtgcagcata ccaagggttg caaacggaaa accaatggcg ggtgccccat
    11521 ctgcaagcag ctcattgccc tctgctgcta ccatgccaag cactgccagg agaacaaatg
    11581 cccggtgccg ttctgcctaa acatcaagca gaagctccgg cagcaacagc tgcagcaccg
    11641 actacagcag gcccaaatgc ttcgcaggag gatggccagc atgcagcgga ctggtgtggt
    11701 tgggcagcaa cagggcctcc cttcccccac tcctgccact ccaacgacac caactggcca
    11761 acagccaacc accccgcaga cgccccagcc cacttctcag cctcagccta cccctcccaa
    11821 tagcatgcca ccctacttgc ccaggactca agctgctggc cctgtgtccc agggtaaggc
    11881 agcaggccag gtgacccctc caacccctcc tcagactgct cagccacccc ttccagggcc
    11941 cccacctgca gcagtggaaa tggcaatgca gattcagaga gcagcggaga cgcagcgcca
    12001 gatggcccac gtgcaaattt ttcaaaggcc aatccaacac cagatgcccc cgatgactcc
    12061 catggccccc atgggtatga acccacctcc catgaccaga ggtcccagtg ggcatttgga
    12121 gccagggatg ggaccgacag ggatgcagca acagccaccc tggagccaag gaggattgcc
    12181 tcagccccag caactacagt ctgggatgcc aaggccagcc atgatgtcag tggcccagca
    12241 tggtcaacct ttgaacatgg ctccacaacc aggattgggc caggtaggta tcagcccact
    12301 caaaccaggc actgtgtctc aacaagcctt acaaaacctt ttgcggactc tcaggtctcc
    12361 cagctctccc ctgcagcagc aacaggtgct tagtatcctt cacgccaacc cccagctgtt
    12421 ggctgcattc atcaagcagc gggctgccaa gtatgccaac tctaatccac aacccatccc
    12481 tgggcagcct ggcatgcccc aggggcagcc agggctacag ccacctacca tgccaggtca
    12541 gcagggggtc cactccaatc cagccatgca gaacatgaat ccaatgcagg cgggcgttca
    12601 gagggctggc ctgccccagc agcaaccaca gcagcaactc cagccaccca tgggagggat
    12661 gagcccccag gctcagcaga tgaacatgaa ccacaacacc atgccttcac aattccgaga
    12721 catcttgaga cgacagcaaa tgatgcaaca gcagcagcaa cagggagcag ggccaggaat
    12781 aggccctgga atggccaacc ataaccagtt ccagcaaccc caaggagttg gctacccacc
    12841 acagcagcag cagcggatgc agcatcacat gcaacagatg caacaaggaa atatgggaca
    12901 gataggccag cttccccagg ccttgggagc agaggcaggt gccagtctac aggcctatca
    12961 gcagcgactc cttcagcaac agatggggtc ccctgttcag cccaacccca tgagccccca
    13021 gcagcatatg ctcccaaatc aggcccagtc cccacaccta caaggccagc agatccctaa
    13081 ttctctctcc aatcaagtgc gctctcccca gcctgtccct tctccacggc cacagtccca
    13141 gcccccccac tccagtcctt ccccaaggat gcagcctcag ccttctccac accacgtttc
    13201 cccacagaca agttccccac atcctggact ggtagctgcc caggccaacc ccatggaaca
    13261 agggcatttt gccagcccgg accagaattc aatgctttct cagcttgcta gcaatccagg
    13321 catggcaaac ctccatggtg caagcgccac ggacctggga ctcagcaccg ataactcaga
    13381 cttgaattca aacctctcac agagtacact agacatacac ctcgaggatc gattaagtag
    13441 ctaactagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
    13501 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
    13561 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
    13621 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
    13681 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
    13741 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
    13801 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
    13861 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
    13921 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
    13981 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
    14041 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
    14101 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
    14161 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
    14221 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
    14281 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
    14341 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
    14401 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    14461 ctgcctctga gc
//
