LOCUS       sequence-375010-        7749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses red chromoprotein on a broad host range RSF1010 origin 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Elston KM, Phillips LE, Leonard SP, Young E, Holley JC, Ahsanullah 
            T, McReynolds B, Moran NA, Barrick JE
  TITLE     The Pathfinder plasmid toolkit for genetically engineering newly 
            isolated bacteria enables the study of Drosophila-colonizing 
            Orbaceae.
  JOURNAL   ISME Commun. 2023 May 24;3(1):49. doi: 10.1038/s43705-023-00255-3.
  PUBMED    37225918
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ISME 
            Commun."; date: "2023-05-24"; volume: "3(1):49. doi"; pages: " 
            10.1038/s43705-023-00255-3"
COMMENT     Sequence Label: pBTK1006 (pSL5)
FEATURES             Location/Qualifiers
     source          1..7749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            1479..1566
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      1908..2302
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             2965..3642
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      3662..3733
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3749..3776
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(4830..5681)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(5668..6507)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(join(7018..7749,1..240))
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
ORIGIN
        1 gctgaggtcg tccaccagca ccagaccatg ccgctcctgc tcggcgggcc tgatatacac
       61 gtcattgccc tgggcattca tccgcttgag ccatggcgtg ttctggagca cttcggcggc
      121 tgaccattcc cggttcatca tctggccggt ggtggcgtcc ctgacgccga tatcgaagcg
      181 ctcacagccc atggccttga gctgtcggcc tatggcctgc aaagtcctgt cgttcttcat
      241 cgggccacca agcgcagcca gatcgagccg tcctcggttg tcagtggcgt caggtcgagc
      301 aagagcaacg atgcgatcag cagcaccacc gtaggcatca tggaagccag catcacggtt
      361 agccatagct tccagtgcca cccccgcgac gcgctccggg cgctctgcgc ggcgctgctc
      421 acctcggcgg ctacctcccg caactctttg gccagctcca cccatgccgc ccctgtctgg
      481 cgctgggctt tcagccactc cgccgcctgc gcctcgctgg cctgctgggt ctggctcatg
      541 acctgccggg cttcgtcggc cagtgtcgcc atgctctggg ccagcggttc gatctgctcc
      601 gctaactcgt tgatgcctct ggatttcttc actctgtcga ttgcgttcat ggtctattgc
      661 ctcccggtat tcctgtaagt cgatgatctg ggcgttggcg gtgtcgatgt tcagggccac
      721 gtctgcccgg tcggtgcgga tgccccggcc ttccatctcc accacgttcg gccccaggtg
      781 aacaccgggc aggcgctcga tgccctgcgc ctcaagtgtt ctgtggtcaa tgcgggcgtc
      841 gtggccagcc cgctctaatg cccggttggc atggtcggcc catgcctcgc gggtctgctc
      901 aagccatgcc ttgggcttga gcgcttcggt cttctgtgcc ccgcccttct ccggggtctt
      961 gccgttgtac cgcttgaacc actgagcggc gggccgctcg atgccgtcat tgatccgctc
     1021 ggagatcatc aggtggcagt gcgggttctc gccgccaccg gcatggatgg ccagcgtata
     1081 cggcaggcgc tcggcaccgg tcaggtgctg ggcgaactcg gacgccagcg ccttctgctg
     1141 gtcgagggtc agctcgaccg gcagggcaaa ttcgacctcc ttgaacagcc gcccattggc
     1201 gcgttcatac aggtcggcag catcccagta gtcggcgggc cgctcgacga actccggcat
     1261 gtgcccggat tcggcgtgca agacttcatc catgtcgcgg gcatacttgc cttcgcgctg
     1321 gatgtagtcg gccttggccc tggccgattg gccgcccgac ctgctgccgg ttttcgccgt
     1381 aaggtgataa atcgccatgc tgcctcgctg ttgcttttgc ttttcggctc catgcaatgg
     1441 ccctcggaga gcgcaccgcc cgaagggtgg ccgttaggcc agtttctcga agagaaaccg
     1501 gtaagtgcgc cctcccctac aaagtagggt cgggattgcc gccgctgtgc ctccatgata
     1561 gcctacgaga cagcacatta acaatggggt gtcaagatgg ttaaggggag caacaaggcg
     1621 gcggatcggc tggccaagct cgaagaacaa cgagcgcgaa tcaatgccga aattcagcgg
     1681 gtgcgggcaa gggaacagca gcaagagcgc aagaacgaaa caaggcgcaa ggtgctggtg
     1741 ggggccatga ttttggccaa ggtgaacagc agcgagtggc cggaggatcg gctcatggcg
     1801 gcaatggatg cgtaccttga acgcgaccac gaccgcgcct tgttcggtct gccgccacgc
     1861 cagaaggatg agccgggctg aatgatcgac cgagacaggc cctgcggggc tgcacacgcg
     1921 cccccaccct tcgggtaggg ggaaaggccg ctaaagcggc taaaagcgct ccagcgtatt
     1981 tctgcggggt ttggtgtggg gtttagcggg ctttgcccgc ctttccccct gccgcgcagc
     2041 ggtggggcgg tgtgtagcct agcgcagcga atagaccagc tatccggcct ctggccgggc
     2101 atattgggca agggcagcag cgccccacaa gggcgctgat aaccgcgcct agtggattat
     2161 tcttagataa tcatggatgg atttttccaa caccccgcca gcccccgccc ctgctgggtt
     2221 tgcaggtttg ggggcgtgac agttattgca ggggttcgtg acagttattg caggggggcg
     2281 tgacagttat tgcaggggtt cgtgacagtt agtacgggag tgacgggcac tggctggcaa
     2341 tgtctagcaa cggcaggcat ttcggctgag ggtaaaagaa ctttccgcta agcgatagac
     2401 tgtatgtaaa cacagtattg caaggacgcg gaacatgcct catgtggcgg ccaggacggc
     2461 cagccgggat cgggatactg gtcgttacca gagccaccga cccgagcaaa cccttctcta
     2521 tcagatcgtt gacgagtatt acccggcatt cgctgcgctt atggcagagc agggaaagga
     2581 attgccgggc tatgtgcaac gggaatttga agaatttctc caatgcgggc ggctggagca
     2641 tggctttcta cgggttcgct gcgagtcttg ccacgccgag cacctggtcg ctttcagaaa
     2701 tcaatctaaa gtatatatga gtaaacttgg tctgacaggc cccttgagac cagctttggc
     2761 agtttattct tgacatgtag tgagggggct ggtataatca catagtactg ttatacagaa
     2821 acagaggaga tattacatat ggcttcctcc gaagacgtta tcaggcccct tgagaccagc
     2881 tttggcagtt tattcttgac atgtagtgag ggggctggta taatcacata gtactgttat
     2941 acagaaacag aggagatatt acatatggct tcctccgaag acgttatcaa agagttcatg
     3001 cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa
     3061 ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt
     3121 ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac
     3181 gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa
     3241 tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc
     3301 ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac
     3361 ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg
     3421 gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac
     3481 tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct
     3541 tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa
     3601 cagtacgaac gtgctgaagg tcgtcactcc accggcgctt aatataagga tctccaggca
     3661 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     3721 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     3781 taagtcggtc tcaccgagcg gccgcttatt tcctcccgtt aaataataga taactattaa
     3841 aaatagacaa tacttgctca taagtaacgg tacttaaatt gtttactttg gcgtgtttca
     3901 ttgcttgatg aaactgattt ttagtaaaca gttgacgata ttctcgattg acccattttg
     3961 aaacaaagta cgtatatagc ttccaatatt tatctggaac atctgtggta tggcgggtaa
     4021 gttttattaa gacactgttt acttttggtt taggatgaaa gcattccgct ggcagcttaa
     4081 gcaattgctg aatcgagact tgagtgtgca agagcaaccc tagtgttcgg tgaatatcca
     4141 aggtacgctt gtagaatcct tcttcaacaa tcagatagat gtcagacgca tggctttcaa
     4201 aaaccacttt tttaataatt tgtgtgctta aatggtaagg aatactccca acaattttat
     4261 acctctgttt gttagggaat tgaaactgta gaatatcttg gtgaattaaa gtgacacgag
     4321 tattcagttt taatttttct gacgataagt tgaatagatg actgtctaat tcaatagacg
     4381 ttacctgttt acttatttta gccagtttcg tcgttaaatg ccctttacct gttccaattt
     4441 cgtaaacggt atcggtttct tttaaattca attgttttat tatttggttg agtacttttt
     4501 cactcgttaa aaagttttga gaatatttta tatttttgtt catgtaatca ctccttctta
     4561 attacaaatt tttagcatct aatttaactt caattcctat tatacaaaat tttaagatac
     4621 tgcactatca acacactctt aagtttgctt ctaagtctta tttccataac ttcttttacg
     4681 tttccgccat tctttgctgt ttcgattttt atgatatggt gcaagtcagc acgaacacga
     4741 accgtcttat ctcccattat atcttttttt gcactgattg gtgtatcatt tcgtttttct
     4801 ttttcatgac caaaatccct taacgtgagt cagcctgccg ccttgggccg ggtgatgtcg
     4861 tacttgcccg ccgcgaactc ggttaccgtc cagcccagcg cgaccagctc cggcaacgcc
     4921 tcgcgcaccc gctggcggcg cttgcgcatg gtcgaaccac tggcctctga cggccagaca
     4981 tagccgcaca aggtatctat ggaagccttg ccggttttgc cggggtcgat ccagccacac
     5041 agccgctggt gcagcaggcg ggcggtttcg ctgtccagcg cccgcacctc gtccatgctg
     5101 atgcgcacat gctggccgcc acccatgacg gcctgcgcga tcaaggggtt cagggccacg
     5161 tacaggcgcc cgtccgcctc gtcgctggcg tactccgaca gcagccgaaa cccctgccgc
     5221 ttgcggccat tctgggcgat gatggatacc ttccaaaggc gctcgatgca gtcctgtatg
     5281 tgcttgagcg ccccaccact atcgacctct gccccgattt cctttgccag cgcccgatag
     5341 ctacctttga ccaccatggc atcagcggtg acggcctccc acttgggttc caggaacagc
     5401 cggagctgcc gtccgccttc ggtcttgggt tccgggccaa gcactaggcc attaggccca
     5461 gccatggcca ccagcccttg caggatgcgc agatcatcag cgcccagcgg ctccgggccg
     5521 ctgaactcga tccgcttgcc gtcgccgtag tcatacgtca cgtccagctt gctgcgcttg
     5581 cgctcgcccc gcttgagggc acggaacagg ccgggggcca gacagtgcgc cgggtcgtgc
     5641 cggacgtggc tgaggctgtg cttgttctta ggcttcacca cggggcaccc ccttgctctt
     5701 gcgctgcctc tccagcacgg cgggcttgag caccccgccg tcatgccgcc tgaaccaccg
     5761 atcagcgaac ggtgcgccat agttggcctt gctcacaccg aagcggacga agaaccggcg
     5821 ctggtcgtcg tccacacccc attcctcggc ctcggcgctg gtcatgctcg acaggtagga
     5881 ctgccagcgg atgttatcga ccagtaccga gctgccccgg ctggcctgct gctggtcgcc
     5941 tgcgcccatc atggccgcgc ccttgctggc atggtgcagg aacacgatag agcacccggt
     6001 atcggcggcg atggcctcca tgcgaccgat gacctgggcc atggggccgc tggcgttttc
     6061 ttcctcgatg tggaaccggc gcagcgtgtc cagcaccatc aggcggcggc cctcggcggc
     6121 gcgcttgagg ccgtcgaacc actccggggc catgatgttg ggcaggctgc cgatcagcgg
     6181 ctggatcagc aggccgtcag ccacggcttg ccgttcctcg gcgctgaggt gcgccccaag
     6241 ggcgtgcagg cggtgatgaa tggcggtggg cgggtcttcg gcgggcaggt agatcaccgg
     6301 gccggtgggc agttcgccca cctccagcag atccggcccg cctgcaatct gtgcggccag
     6361 ttgcagggcc agcatggatt taccggcacc accgggcgac accagcgccc cgaccgtacc
     6421 ggccaccatg ttgggcaaaa cgtagtccag cggtggcggc gctgctgcga acgcctccag
     6481 aatattgata ggcttatggg tagccattga ttgcctcctt tgcaggcagt tggtggttag
     6541 gcgctggcgg ggtcactacc cccgccctgc gccgctctga gttcttccag gcactcgcgc
     6601 agcgcctcgt attcgtcgtc ggtcagccag aacttgcgct gacgcatccc tttggccttc
     6661 atgcgctcgg catatcgcgc ttggcgtaca gcgtcagggc tggccagcag gtcgccggtc
     6721 tgcttgtcct tttggtcttt catatcagtc accgagaaac ttgccggggc cgaaaggctt
     6781 gtcttcgcgg aacaaggaca aggtgcagcc gtcaaggtta aggctggcca tatcagcgac
     6841 tgaaaagcgg ccagcctcgg ccttgtttga cgtataacca aagccaccgg gcaaccaata
     6901 gcccttgtca cttttgatca ggtagaccga ccctgaagcg cttttttcgt attccataaa
     6961 acccccttct gtgcgtgagt actcatagta taacaggcgt gagtaccaac gcaagcacta
     7021 catgctgaaa tctggcccgc ccctgtccat gcctcgctgg cggggtgccg gtgcccgtgc
     7081 cagctcggcc cgcgcaagct ggacgctggg cagacccatg accttgctga cggtgcgctc
     7141 gatgtaatcc gcttcgtggc cgggcttgcg ctctgccagc gctgggctgg cctcggccat
     7201 ggccttgccg atttcctcgg cactgcggcc ccggctggcc agcttctgcg cggcgataaa
     7261 gtcgcacttg ctgaggtcat caccgaagcg cttgaccagc ccggccatct cgctgcggta
     7321 ctcgtccagc gccgtgcgcc ggtggcggct aagctgccgc tcgggcagtt cgaggctggc
     7381 cagcctgcgg gccttctcct gctgccgctg ggcctgctcg atctgctggc cagcctgctg
     7441 caccagcgcc gggccagcgg tggcggtctt gcccttggat tcacgcagca gcacccacgg
     7501 ctgataaccg gcgcgggtgg tgtgcttgtc cttgcggttg gtgaagcccg ccaagcggcc
     7561 atagtggcgg ctgtcggcgc tggccgggtc ggcgtcgtac tcgctggcca gcgtccgggc
     7621 aatctgcccc cgaagttcac cgcctgcggc gtcggccacc ttgacccatg cctgatagtt
     7681 cttcgggctg gtttccacta ccagggcagg ctcccggccc tcggctttca tgtcatccag
     7741 gtcaaactc
//
