LOCUS       sequence-37504-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Subach FV, Patterson GH, Renz M, Lippincott-Schwartz J, Verkhusha VV
  TITLE     Bright monomeric photoactivatable red fluorescent protein for 
            two-color super-resolution sptPALM of live cells.
  JOURNAL   J Am Chem Soc. 2010 May 12;132(18):6481-91.
  PUBMED    20394363
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc."; date: "2010-05-12"; volume: "132(18)"; pages: "6481-91"
COMMENT     Sequence Label: pPATagRFP-N1
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..98
                     /label=MCS
                     /note="multiple cloning site"
     CDS             106..807
                     /codon_start=1
                     /product="photoactivatable variant of the monomeric red 
                     fluorescent protein TagRFP (Subach et al., 2010)"
                     /label=PA-TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSSTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMKKKTLGWEPSTEKLKPADGGLEGRVDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDRRLEIIKEADKETYWEQHEVAVARYS
                     DLPSKLGHKLN"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt
       61 ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatgag cgagctgatt
      121 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag
      181 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg
      241 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc
      301 agcagcacct tcatcaacca cacccagggc atccccgact tctggaagca gtccttccct
      361 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc
      421 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
      481 ttcccatcca acggccctgt gatgaagaag aaaacactcg gctgggaacc gagcaccgag
      541 aaactgaaac ccgctgacgg cggcctggaa ggcagggtcg acatggccct gaagctcgtg
      601 ggcgggggcc acctgatctg caacttcaag accacataca gatccaagaa acccgctaag
      661 aacctcaaga tgcccggcgt ctactatgtg gaccgcagac tggaaatcat caaggaggcc
      721 gacaaagaga cctactggga gcagcacgag gtggctgtgg ccagatactc cgacctccct
      781 agcaaactgg ggcacaagct taattaaagc ggccgcgact ctagatcata atcagccata
      841 ccacatttgt aga
//
