LOCUS       sequence-375046-        6217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Polycistronic human LIN28 and NANOG were cloned into the pMXs 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Wang L, Su Y, Huang C, Yin Y, Chu A, Knupp A, Tang Y
  TITLE     NANOG and LIN28 dramatically improve human cell reprogramming by 
            modulating LIN41 and canonical WNT activities.
  JOURNAL   Biol Open. 2019 Dec 5;8(12). pii: 8/12/bio047225. doi: 
            10.1242/bio.047225.
  PUBMED    31806618
REFERENCE   2  (bases 1 to 6217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Open."; date: "2019-12-5"; volume: "8(12). pii: 8/12/bio047225. 
            doi"; pages: " 10.1242/bio.047225"
COMMENT     Sequence Label: pMXs-LN
FEATURES             Location/Qualifiers
     source          1..6217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..594
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    657..1014
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1073..1489
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1419..1441
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1457..1473
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1499..1873
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1811..1827
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             2538..2594
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             3701..4170
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4300..4317)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4471..5059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4551..4570)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5223..6014,6015..6083))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5846..5865
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6084..6188)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaaat acataactga gaatagaaaa gttcagatca aggtcaggaa cagatggaac
      121 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
      181 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
      241 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
      301 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
      361 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
      421 ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgattgact gagtcgcccg
      481 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
      541 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
      601 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
      661 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc
      721 ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
      781 gagttcggaa cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt
      841 tgtggcccga cctgagtcca aaaatcccga tcgttttgga ctctttggtg caccccccta
      901 ataggaggga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
      961 tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat
     1021 cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt agggccagac
     1081 tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca
     1141 caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc
     1201 aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt
     1261 taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt
     1321 gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa
     1381 gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac
     1441 cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccc ccatatggcc
     1501 atatgagatc ttatatgggg cacccccgcc ccttgtaaac ttccctgacc ctgacatgac
     1561 aagagttact aacagcccct ctctccaagc tcacttacag gctctctact tagtccagca
     1621 cgaagtctgg agacctctgg cggcagccta ccaagaacaa ctggaccgac cggtggtacc
     1681 tcacccttac cgagtcggcg acacagtgtg ggtccgccga caccagacta agaacctaga
     1741 acctcgctgg aaaggacctt acacagtcct gctgaccacc cccaccgccc tcaaagtaga
     1801 cggcatcgca gcttggatac acgccgccca cgtgaaggct gccgaccccg ggggtggacc
     1861 atcctctaga ctgccggatc tagctagtta attaaggatc catgggctcc gtgtccaacc
     1921 agcagtttgc aggtggctgc gccaaggcgg cagaagaggc gcccgaggag gcgccggagg
     1981 acgcggcccg ggcggcggac gagcctcagc tgctgcacgg tgcgggcatc tgtaagtggt
     2041 tcaacgtgcg catggggttc ggcttcctgt ccatgaccgc ccgcgccggg gtcgcgctcg
     2101 accccccagt ggatgtcttt gtgcaccaga gtaagctgca catggaaggg ttccggagct
     2161 tgaaggaggg tgaggcagtg gagttcacct ttaagaagtc agccaagggt ctggaatcca
     2221 tccgtgtcac cggacctggt ggagtattct gtattgggag tgagaggcgg ccaaaaggaa
     2281 agagcatgca gaagcgcaga tcaaaaggag acaggtgcta caactgtgga ggtctagatc
     2341 atcatgccaa ggaatgcaag ctgccacccc agcccaagaa gtgccacttc tgccagagca
     2401 tcagccatat ggtagcctca tgtccgctga aggcccagca gggccctagt gcacagggaa
     2461 agccaaccta ctttcgagag gaagaagaag aaatccacag ccctaccctg ctcccggagg
     2521 cacagaatgg atctggagca acaaacttct cactactcaa acaagcaggt gacgtggagg
     2581 agaatcccgg ccctatgagt gtggatccag cttgtcccca aagcttgcct tgctttgaag
     2641 catccgactg taaagaatct tcacctatgc ctgtgatttg tgggcctgaa gaaaactatc
     2701 catccttgca aatgtcttct gctgagatgc ctcacacgga gactgtctct cctcttccct
     2761 cctccatgga tctgcttatt caggacagcc ctgattcttc caccagtccc aaaggcaaac
     2821 aacccacttc tgcagagaat agtgtcgcaa aaaaggaaga caaggtccca gtcaagaaac
     2881 agaagaccag aactgtgttc tcttccaccc agctgtgtgt actcaatgat agatttcaga
     2941 gacagaaata cctcagtctc cagcagatgc aagaactctc caacatcctg aacctcagct
     3001 acaaacaggt gaagacctgg ttccagaacc agagaatgaa atctaagagg tggcagaaaa
     3061 acaactggcc gaagaatagc aatggtgtga cgcagaaggc ctcagcacct acctacccca
     3121 gcctctactc ttcctaccac cagggatgcc tggtgaaccc gactgggaac cttccaatgt
     3181 ggagcaacca gacctggaac aattcaacct ggagcaacca gacccagaac atccagtcct
     3241 ggagcaacca ctcctggaac actcagacct ggtgcaccca atcctggaac aatcaggcct
     3301 ggaacagtcc cttctataac tgtggagagg aatctctgca gtcctgcatg cagttccagc
     3361 caaattctcc tgccagtgac ttggaggctg ctttggaagc tgctggggaa ggccttaatg
     3421 taatacagca gaccactagg tattttagta ctccacaaac catggattta ttcctaaact
     3481 actccatgaa catgcaacct gaagacgtgt gagcggccgc cagcacagtg gtcgacgata
     3541 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
     3601 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga
     3661 atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg
     3721 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa
     3781 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     3841 gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca
     3901 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
     3961 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
     4021 actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac
     4081 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt
     4141 gattgactac ccgtcagcgg gggtctttca catgcagcat gtatcaaaat taatttggtt
     4201 ttttttctta agtatttaca ttaaatggcc atagttgcat taatgaatcg gccaacgcgc
     4261 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     4321 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     4381 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     4441 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     4501 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     4561 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     4621 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     4681 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4741 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4801 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4861 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     4921 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4981 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     5041 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5101 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     5161 gatccttttg cggccggccg caaatcaatc taaagtatat atgagtaaac ttggtctgac
     5221 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5281 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5341 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     5401 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5461 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     5521 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5581 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5641 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5701 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5761 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5821 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5881 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5941 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6001 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     6061 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     6121 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6181 gttccgcgca catttccccg aaaagtgcca cctgcat
//
