LOCUS       sequence-375052-        9922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9922)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 9922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-CMV-Galpha-i3 ONE-GO
FEATURES             Location/Qualifiers
     source          1..9922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(679..681,682..684,685..1395)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(724..745)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(985..1004)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1332..1353
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2135..2709,2710..2712,2713..2721)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2302..2319)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2529..2548
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2732..2752
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             2858..3373
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    3387..3975
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3440..3460)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3858..3869)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4182..4815
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4943..4959)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4943..4959)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4956..4978)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4967..4983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4991..4997,4998..5015,5016..5021))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5036..5057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5174..5191)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5345..5933)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5425..5444)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6104..6895,6896..6964))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6727..6746
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6965..7069)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7117..7251
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7154..7173)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7208..7227
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7419..8052
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8099..8224
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8721..8954
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9139..9183
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9446..9561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        9619..9922
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgactctact agaggatcta tttccggtga
      301 attcctcgag actagttcta gagcggccgc ggatccatgg gctgcacgtt gagcgccgag
      361 gacaaggcgg cggtggagcg gagtaagatg atcgaccgca acttgcggga ggacggagag
      421 aaagcggcca aagaagtgaa gctgctgctg ctcggcgctg gagaatctgg taaaagtact
      481 attgtgaaac agatgaaaat cattcatgag gatggctatt ccgaggacga gtgtaagcag
      541 tataaagttg tcgtctacag caataccatt cagtccatca ttgcaatcat aagagccatg
      601 ggacggttga agattgattt tggggaagct gccagagcgg atgatgcccg acagttattt
      661 gttttagctg gcagcgctat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      721 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      781 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      841 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc
      901 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      961 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1021 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1081 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     1141 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     1201 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1261 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
     1321 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1381 gacgagctgt acaagagcgc tgaggaagga gtcatgactt cagaactagc aggcgtgatt
     1441 aaacgtttat ggcgagatgg cggggtgcag gcatgcttca gcaggtccag ggaatatcag
     1501 ctcaatgatt ctgcttcata ttacctaaat gatttggata gaatatccca gaccaactac
     1561 attccaactc agcaagatgt tcttcggacg agagtgaaga ctacaggcat tgtggagacc
     1621 cacttcacct tcaaggagct ctacttcaaa atgtttgatg taggtggcca aagatccgaa
     1681 cggaaaaagt ggatccactg ttttgaggga gtgacagcaa ttatcttttg tgtggctctc
     1741 agtgattacg accttgttct ggctgaggac gaggaaatga accgaatgca tgaaagcatg
     1801 aaattgtttg acagcatttg taacaacaaa tggtttacag acacttcaat cattctcttc
     1861 cttaataaga aagacctttt tgaggaaaaa ataaagagga gtccattaac aatctgttat
     1921 ccagaataca caggttccaa tacgtacgaa gaggcagctg cttacatcca gtgccagttt
     1981 gaagatctga accgaagaaa ggacaccaag gaggtctaca ctcactttac ctgtgccact
     2041 gacaccaaaa acgtgcagtt tgtttttgat gctgttacag atgtcatcat taaaaacaac
     2101 ttaaaggaat gtgggcttta ctgagcggcc gccgcccctc tccctccccc ccccctaacg
     2161 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     2221 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     2281 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     2341 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     2401 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     2461 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     2521 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     2581 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     2641 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     2701 gatgataagc ttgccacaac ccgtaccaaa gatggggagt agcaagagca agacgtcgaa
     2761 ttccggaatc aagcttggtg gatcttctcg aggttactac catggtattt gggtgggtga
     2821 agaaggtcga ctttctcgag gtggcaccgg tggatccatg gtcttcacac tcgaagattt
     2881 cgttggggac tggcgacaga cagccggcta caacctggac caagtccttg aacagggagg
     2941 tgtgtccagt ttgtttcaga atctcggggt gtccgtaact ccgatccaaa ggattgtcct
     3001 gagcggtgaa aatgggctga agatcgacat ccatgtcatc atcccgtatg aaggtctgag
     3061 cggcgaccaa atgggccaga tcgaaaaaat ttttaaggtg gtgtaccctg tggatgatca
     3121 tcactttaag gtgatcctgc actatggcac actggtaatc gacggggtta cgccgaacat
     3181 gatcgactat ttcggacggc cgtatgaagg catcgccgtg ttcgacggca aaaagatcac
     3241 tgtaacaggg accctgtgga acggcaacaa aattatcgac gagcgcctga tcaaccccga
     3301 cggctccctg ctgttccgag taaccatcaa cggagtgacc ggctggcggc tgtgcgaacg
     3361 cattctggcg taaacgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat
     3421 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     3481 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     3541 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     3601 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     3661 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     3721 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     3781 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     3841 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     3901 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     3961 gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat
     4021 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     4081 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     4141 agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc
     4201 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     4261 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     4321 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     4381 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     4441 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     4501 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     4561 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     4621 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     4681 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     4741 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     4801 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     4861 agaaatgaat atcagagagt gagaggcctt gacattgcta gcgtttaccg tcgacctcta
     4921 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     4981 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     5041 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     5101 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     5161 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     5221 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     5281 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5341 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5401 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5461 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5521 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5581 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5641 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5701 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5761 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5821 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5881 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5941 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6001 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6061 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6121 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     6181 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     6241 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6301 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6361 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     6421 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6481 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6541 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6601 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6661 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6721 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6781 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6841 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6901 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6961 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7021 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7081 gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat
     7141 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7201 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
     7261 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
     7321 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
     7381 aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca
     7441 aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
     7501 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
     7561 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
     7621 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
     7681 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
     7741 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
     7801 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
     7861 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7921 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7981 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8041 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     8101 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     8161 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     8221 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     8281 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     8341 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     8401 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     8461 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     8521 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc
     8581 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     8641 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     8701 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     8761 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     8821 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     8881 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     8941 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     9001 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     9061 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     9121 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     9181 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     9241 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     9301 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     9361 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     9421 gtgaacggat ctcgacggta tcgcctttaa aagaaaaggg gggattgggg ggtacagtgc
     9481 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     9541 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttatcg
     9601 ataagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     9661 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     9721 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     9781 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     9841 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     9901 attagtcatc gctattacca tg
//
