LOCUS       sequence-375056-       10366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human KIF1C with P176L mutation and mCherry 
            and FRB tags..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10366)
  AUTHORS   Siddiqui N, Roth D, Toleikis A, Zwetsloot AJ, Cross RA, Straube A
  TITLE     Force generation of KIF1C is impaired by pathogenic mutations.
  JOURNAL   Curr Biol. 2022 Sep 12;32(17):3862-3870.e6. doi: 
            10.1016/j.cub.2022.07.029. Epub 2022 Aug 11.
  PUBMED    35961316
REFERENCE   2  (bases 1 to 10366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2022.07.029"; journalName: "Curr Biol"; date: 
            "2022-09-12- 12"; volume: "32"; issue: "17"; pages: "3862-3870.e6"
COMMENT     Sequence Label: pKIF1C_P176L-mCherry-FRB
FEATURES             Location/Qualifiers
     source          1..10366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1069..1091
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      3411..3420
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4503..4512
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4509..5216
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4654..4672)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4922..4941
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5223..5501
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     polyA_signal    5700..5834
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5737..5756)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5791..5810
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5848..5864)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5848..5864)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5861..5883)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5872..5888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5896..5902,5903..5920,5921..5926))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5941..5962
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6079..6096)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6250..6838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6330..6349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7009..7800,7801..7869))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7632..7651
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7870..7974)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8042..8060
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8098..8120)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8220..8239
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8433..8455
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8447..8464
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8448..8464
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gggcggggsg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga
       61 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
      121 cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc
      181 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
      241 tctcctccgg gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg
      301 cgtgaaagcc ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg
      361 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
      421 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
      481 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
      541 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc
      601 ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg
      661 cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg
      721 gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga
      781 gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg
      841 agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc
      901 gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg
      961 gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg
     1021 gctgtccgca gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct
     1081 ggcgtgtgac cggcggggtt tatatcttcc cttctctgtt cctccgcagc ccccaagcta
     1141 gcgctaccgg actcagatct cgagctcaag cttcgaattc tggagctatg gctggtgcct
     1201 cggtgaaagt ggcagtgagg gttcggccct ttaacgcccg tgagaccagc caggatgcca
     1261 agtgtgtggt cagcatgcag ggcaacacca cctccatcat caatcctaaa cagagcaagg
     1321 atgcccccaa aagcttcacc tttgactact cctactggtc acacacttcg acggaggacc
     1381 cccagtttgc atctcagcag caagtgtatc gggacatcgg agaagagatg ctgctccacg
     1441 cctttgaagg ctacaacgtg tgcatctttg cctatgggca gaccggggct gggaaatcct
     1501 ataccatgat ggggcgacag gagccagggc agcagggcat cgtgccccag ctctgtgagg
     1561 acctcttctc tcgcgttagt gagaaccaga gtgctcagct atcctactct gtggaggtgt
     1621 cttacatgga aatctactgt gagcgggtac gagacctctt gaaccccaag agtcggggtt
     1681 ctctgcgggt ccgggagcac cccatcctgg gcctgtacgt gcaggacctg tccaaattgg
     1741 ctgtgacctc ctacgcagac attgctgacc tcatggactg tggaaataaa gcacggactg
     1801 tggctgccac caacatgaat gagaccagca gccgttccca tgccgtcttt accatcgtct
     1861 tcacacagcg ctgccatgac cagctcacgg ggctggactc ggagaaggtc agtaagatca
     1921 gtttggtgga ccttgctggg agtgagcgag ccgactcctc aggggcccgg ggcatgcgcc
     1981 tgaaggaagg agccaacatc aataagtccc tgactacact agggaaagtg atctcggccc
     2041 ttgcagatat gcaatcaaag aagcgaaagt cggattttat cccctacagg gactctgtgc
     2101 tcacctggct gctcaaggaa aatttggggg ggaactcacg cacagccatg attgcagccc
     2161 tgagccctgc tgacatcaat tacgaggaga ctctcagcac cctcaggtat gctgaccgca
     2221 ccaagcaaat ccgctgcaat gccatcatca acgaggaccc taatgcccgg ctgattagag
     2281 agctgcagga ggaagtagcc cggctgcggg aactgctgat ggctcaggga ctgtcagcct
     2341 ctgctctgga aggcctgaag acggaagaag ggagtgtcag aggcgccctg ccagctgtgt
     2401 catctccccc agctccagtt tcaccctcat cacccaccac acataatggg gagctggagc
     2461 cgtcattctc ccccaacacg gagtcccaga ttgggcctga ggaagccatg gagaggctgc
     2521 aggagacaga gaagattata gctgagctga acgagacatg ggaggagaag ctacgcaaga
     2581 cagaagccct gaggatggag agagaagcat tgctggctga gatgggggtg gccgtccggg
     2641 aggatggggg aactgtgggc gtcttctctc caaagaagac tccccacctg gtgaacctga
     2701 acgaagaccc tctgatgtct gagtgtctgc tctaccacat caaagatggc gtcaccaggg
     2761 tcggccaagt agatatggac atcaagctga ccggacagtt cattcgggag caacactgtc
     2821 tgttccggag catcccccag ccagatggag aagtggtggt cactctggag ccttgtgaag
     2881 gagctgagac atatgtgaat gggaagcttg tgacggagcc gctggtgctg aagtcaggga
     2941 ataggattgt gatgggcaag aaccacgttt tccgcttcaa ccacccggag caggcaaggc
     3001 tggaacggga acgaggggtc cccccacccc caggaccgcc ctctgagcca gtcgactgga
     3061 actttgccca gaaggaactg ctggagcagc aaggcatcga cataaagctg gaaatggaga
     3121 agaggctgca ggatctggag aatcagtacc ggaaagaaaa ggaagaagcc gatcttctgc
     3181 tggagcagca gcgactgtat gcagactcgg acagcgggga tgactctgac aagcgctctt
     3241 gtgaagagag ctggaggctc atctcctcct tgcgggagca gctgccgccc accacggtcc
     3301 agaccattgt caaacgctgt ggtctgccca gcagtggcaa gcgcagggcc cctcgcaggg
     3361 tttatcagat cccccagcga cgcaggctgc agggcaaaga cccccgctgg gccaccatgg
     3421 ctgacctgaa gatgcaggcg gtgaaggaga tctgctacga ggtggccctg gctgacttcc
     3481 gccacgggcg ggctgagatt gaggccctgg ccgccctcaa gatgcgggag ctgtgtcgca
     3541 cctatggcaa gccagacggc cccggagacg cctggagggc tgtggcccgg gatgtctggg
     3601 acactgtagg cgaggaggaa ggaggtggag ctggcagtgg tggtggcagt gaggagggag
     3661 cccgaggggc ggaggtggag gacctccggg cccacatcga caagctgacg gggattctgc
     3721 aggaggtgaa gctgcagaac agcagcaagg accgggagct gcaggccctg cgggaccgca
     3781 tgctccgcat ggagagggtc atccccctgg cccaggatca tgaggatgag aatgaagaag
     3841 gtggtgaggt cccctgggcc ccgcctgaag gatcagaggc agcagaggag gcagccccca
     3901 gtgaccgcat gccgtcagcc cggcccccct cgccaccact gtcaagctgg gagcgggtgt
     3961 cacggctcat ggaggaggac cctgccttcc gtcgtggtcg tcttcgctgg ctcaagcagg
     4021 agcagctacg gctgcaggga ctgcagggct ctgggggccg gggcgggggg ctgcgcaggc
     4081 ccccagcccg ctttgtgccc cctcacgact gcaagctacg cttccccttc aagagcaacc
     4141 cccagcaccg ggagtcttgg ccagggatgg ggagcgggga ggctccaact ccgctccaac
     4201 cccctgagga ggtcactccc catccagcca cccctgcccg ccggcctccg agtccccgaa
     4261 ggtcccacca tccccgcagg aactccctgg atggaggggg ccgatcccgg ggagcgggtt
     4321 ctgcacagcc tgaaccccag cacttccagc ccaaaaagca caactcttat ccccagccac
     4381 cccaacccta cccagcccag cggcccccag ggccccgcta ccccccatac actactcccc
     4441 cacgaatgag acggcagcgt tctgcccctg acctcaagga gagtggggca gctgtggatc
     4501 ccgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     4561 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     4621 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     4681 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     4741 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     4801 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     4861 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     4921 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     4981 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     5041 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     5101 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     5161 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagacta
     5221 gtatcctctg gcatgagatg tggcatgaag gcctggaaga ggcatctcgt ttgtactttg
     5281 gggaaaggaa cgtgaaaggc atgtttgagg tgctggagcc cttgcatgct atgatggaac
     5341 ggggccccca gactctgaag gaaacatcct ttaatcaggc ctatggtcga gatttaatgg
     5401 aggcccaaga gtggtgcagg aagtacatga aatcagggaa tgtcaaggac ctcctccaag
     5461 cctgggacct ctattatcat gtgttccgac gaatctcaaa gtaagcggcc gccatatgca
     5521 tcctaggcct attaatattc cggagtatac gtagccggct aacgttaaca accggtacct
     5581 ctagataagt aatgatcata atcagccata tcacatctgt agaggtttta cttgctttaa
     5641 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     5701 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5761 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5821 atcatgtctg gatcaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     5881 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5941 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6001 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6061 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6121 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6181 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6241 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6301 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6361 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6421 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6481 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6541 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6601 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6661 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6721 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6781 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6841 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6901 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6961 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7021 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7081 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7141 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7201 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7261 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7321 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7381 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7441 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7501 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7561 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7621 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7681 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7741 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7801 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7861 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7921 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7981 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     8041 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     8101 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     8161 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     8221 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     8281 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     8341 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     8401 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     8461 cagtgccaag ttgggatctt tgcattggcc cacggctctc aggatgggga tgctcccctt
     8521 cagcacccgg ttccccttgg aaactgatgg tcctggctct gtggcatggc agtggcactg
     8581 tgaggagccc cctaccagca gcacacagtg gggttggcac tgccacgctc cggatgccgc
     8641 gctctgatcc aaccccataa tcaagggaac ccgaattgcc ccatcattgc ccccaccacc
     8701 cccatcctgc cgggccctca caccccacgc tgccttctgg tgacattccc cagcccaaac
     8761 ccacggcttc atggctaccg cggggcattt cccattgccg ccccattatc agctctgcac
     8821 acctcccgct gtacccatgc ctcgtggctg cccttctttg acgtataatc ttctaattaa
     8881 tacccggcct tgtcaaagtg gagcacaaac gttaattaat tccccagcag gcaggtaatt
     8941 aacagtgtga ctcccttttt gctgcgagtg gggctgatac agagagatgt ggcactatgg
     9001 agcccacggg gtcctggcac tgggtgccca cggaggtccc catgtgctgc agtgtcaccg
     9061 cctccgagga gacagtattg tccctgcggt gtccctgcag ctcagctctg tccacagggc
     9121 cacctccagt ttggagggga cacaatgcag ccccgatgca acccatcctc gcagcatccc
     9181 agggacaaag accccactgc aagaccgcac acagggctgg gtcccgctcc cctaatatct
     9241 acagtgcttc ttgcatggcc ccttaatcaa tgcagttaat cagcatgcgc tcatgcaccg
     9301 ctctggagct gcaaagcccc tcgcagcgct gctcaccaac accgcgcacc gccccggccc
     9361 agcctgcagc acgcgctgca aacaggaaag aaacaaaata ttgcccaaat gtaggcaaag
     9421 gcattcggct gccttgacct ccgccgggcc gggccctgcc tgactcagct ccttactcag
     9481 cgctcgcttc ctccctccgg ctgccaccgc cgcagcgcac accctgacaa agagtggccc
     9541 ttaacgggct ctgaggtgca cccagcagtg cactcagcag tccaagggcc ggcctggagg
     9601 tttgcaccgc tacgtgctga cattagcatt gaacttggcc ctgggtagtg ctgcaggccg
     9661 ggcggggtgg gtgtagagag tgcagcgcgc gttgcacccg gtgccccttc ccctcccttg
     9721 catcccagca ggctgcaccc cagcaccagg cccgtgcatg catgctcctg gtgttattgc
     9781 agcctggtgc atgcatgcgt cttgatggtg cagcgctgtg catgcatcct ccttggtgtg
     9841 tagcagtcta gtgcatgcat acccctcggt gttattgctg ctctgtgcac gcacgctcat
     9901 tgtatcactt catcccagtg catgcactca cactggagcg attgctgctc ggtgcacgca
     9961 cactcattgt atcactgcag ctcagtggct gcacgcacac cggtgttatt gctgctcggt
    10021 gcgtgcatgc acatcagtgt cgctgcagct cagtgcatgc acgctcattg cccatcgcta
    10081 tccctgcctc tcctgctggc gctccccggg aggtgacttc aaggggaccg caggaccacc
    10141 tcgggggtgg ggggagggct gcacacgcgg accccgctcc ccctccccaa caaagcactg
    10201 tggaatcaaa aaggggggag gggggatgga ggggcgcgtc acacccccgc cccacaccct
    10261 cacctcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
    10321 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgg
//
