LOCUS       sequence-375064-        8255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TdgA vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8255)
  AUTHORS   Ye L, Park JJ, Peng L, Yang Q, Chow RD, Dong MB, Lam SZ, Guo J, Tang
            E, Zhang Y, Wang G, Dai X, Du Y, Kim HR, Cao H, Errami Y, Clark P, 
            Bersenev A, Montgomery RR, Chen S
  TITLE     A genome-scale gain-of-function CRISPR screen in CD8 T cells 
            identifies proline metabolism as a means to enhance CAR-T therapy.
  JOURNAL   Cell Metab. 2022 Apr 5;34(4):595-614.e14. doi: 
            10.1016/j.cmet.2022.02.009. Epub 2022 Mar 10.
  PUBMED    35276062
REFERENCE   2  (bases 1 to 8255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2022.02.009"; journalName: "Cell Metab"; date: 
            "2022-04-5- 5"; volume: "34"; issue: "4"; pages: "595-614.e14"
COMMENT     Sequence Label: 
            pSC014_pLKO-U6-BsmBI-MS2sgRNA-EFS-Thy11-2A-MS2-p65-HSF1
FEATURES             Location/Qualifiers
     source          1..8255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1717..1965
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1717..1737
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1888..1907
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2003..2021
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        2073..2091
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_feature    2184..2301
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2415..2626
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2652..3137
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     CDS             3147..3200
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3204..3590
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             3645..3665
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3681..4223
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             4248..4619
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     polyA_signal    4635..4683
                     /note="synthetic polyadenylation signal"
     LTR             4772..5005
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5083..5204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5120..5139)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5174..5193
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5244..5379
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5399..5418)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5400..5418)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5428..5445)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5428..5444)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5437..5459)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5586..6041
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5673..5692)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5883..5904
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6067..6171
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6172..6240,6241..7032)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6390..6409)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7203..7791
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7692..7711
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7945..7962
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8079..8100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8115..8120,8121..8138,8139..8145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8153..8169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8158..8180
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8177..8193
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8177..8193
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8212..8232
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8214..8232
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagagagg gcctatttcc catgattcct
     1741 tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta
     1801 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     1861 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     1921 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccggaga cgggataccg
     1981 tctctgtttt agagctaggc caacatgagg atcacccatg tctgcagggc ctagcaagtt
     2041 aaaataaggc tagtccgtta tcaacttggc caacatgagg atcacccatg tctgcagggc
     2101 caagtggcac cgagtcggtg ctttttttgg atccaagctt ggcgtaacta gatcttgaga
     2161 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     2221 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     2281 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc
     2341 cggctcgagg gggcccgggg aattcgctag ctaggtcttg aaaggagtgg gaattggctc
     2401 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     2461 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     2521 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     2581 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggacc ggttctagac
     2641 gtacggccac catgaaccca gccatcagcg tcgctctcct gctctcagtc ttgcaggtgt
     2701 cccgagggca gaaggtgacc agcctgacag cctgcctggt gaaccaaaac cttcgcctgg
     2761 actgccgcca tgagaataac accaaggata actccatcca gcatgagttc agcctgaccc
     2821 gagagaagag gaagcacgtg ctctcaggca cccttgggat acccgagcac acgtaccgct
     2881 cccgcgtcac cctctccaac cagccctata tcaaggtcct taccctagcc aacttcacca
     2941 ccaaggatga gggcgactac ttttgtgagc ttcgcgtgtc gggcgcgaat cccatgagct
     3001 ccaataaaag tatcagtgtg tatagagaca agctggtcaa gtgtggcggc ataagcctgc
     3061 tggttcagaa cacatcctgg atgctgctgc tgctgctttc cctctccctc ctccaagccc
     3121 tggacttcat ttctctgggc agtggagagg gcagaggaag tctgctaaca tgcggtgacg
     3181 tcgaggagaa tcctggccca atggcttcaa actttactca gttcgtgctc gtggacaatg
     3241 gtgggacagg ggatgtgaca gtggctcctt ctaatttcgc taatggggtg gcagagtgga
     3301 tcagctccaa ctcacggagc caggcctaca aggtgacatg cagcgtcagg cagtctagtg
     3361 cccagaagag aaagtatacc atcaaggtgg aggtccccaa agtggctacc cagacagtgg
     3421 gcggagtcga actgcctgtc gccgcttgga ggtcctacct gaacatggag ctcactatcc
     3481 caattttcgc taccaattct gactgtgaac tcatcgtgaa ggcaatgcag gggctcctca
     3541 aagacggtaa tcctatccct tccgccatcg ccgctaactc aggtatctac agcgctggag
     3601 gaggtggaag cggaggagga ggaagcggag gaggaggtag cggacctaag aaaaagagga
     3661 aggtggcggc cgctggatcc ccttcagggc agatcagcaa ccaggccctg gctctggccc
     3721 ctagctccgc tccagtgctg gcccagacta tggtgccctc tagtgctatg gtgcctctgg
     3781 cccagccacc tgctccagcc cctgtgctga ccccaggacc accccagtca ctgagcgctc
     3841 cagtgcccaa gtctacacag gccggcgagg ggactctgag tgaagctctg ctgcacctgc
     3901 agttcgacgc tgatgaggac ctgggagctc tgctggggaa cagcaccgat cccggagtgt
     3961 tcacagatct ggcctccgtg gacaactctg agtttcagca gctgctgaat cagggcgtgt
     4021 ccatgtctca tagtacagcc gaaccaatgc tgatggagta ccccgaagcc attacccggc
     4081 tggtgaccgg cagccagcgg ccccccgacc ccgctccaac tcccctggga accagcggcc
     4141 tgcctaatgg gctgtccgga gatgaagact tctcaagcat cgctgatatg gactttagtg
     4201 ccctgctgtc acagatttcc tctagtgggc agggaggagg tggaagcggc ttcagcgtgg
     4261 acaccagtgc cctgctggac ctgttcagcc cctcggtgac cgtgcccgac atgagcctgc
     4321 ctgaccttga cagcagcctg gccagtatcc aagagctcct gtctccccag gagcccccca
     4381 ggcctcccga ggcagagaac agcagcccgg attcagggaa gcagctggtg cactacacag
     4441 cgcagccgct gttcctgctg gaccccggct ccgtggacac cgggagcaac gacctgccgg
     4501 tgctgtttga gctgggagag ggctcctact tctccgaagg ggacggcttc gccgaggacc
     4561 ccaccatctc cctgctgaca ggctcggagc ctcccaaagc caaggacccc actgtctcct
     4621 aatgtacaag cgctaataaa agatctttat tttcattaga tctgtgtgtt ggttttttgt
     4681 gtggtaactc tagacgtgcg gtcgacttta agaccaatga cttacaaggc agctgtagat
     4741 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     4801 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     4861 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     4921 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     4981 tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag
     5041 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     5101 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5161 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     5221 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5281 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     5341 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     5401 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     5461 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     5521 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     5581 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5641 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5701 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5761 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     5821 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5881 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     5941 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6001 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     6061 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6121 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6181 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6241 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6301 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6361 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     6421 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6481 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6541 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6601 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6661 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6721 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6781 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6841 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6901 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6961 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7021 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7081 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7141 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7201 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7261 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7321 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     7381 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7441 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7501 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7561 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7621 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7681 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7741 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7801 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7861 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7921 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     7981 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     8041 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     8101 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     8161 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     8221 cctcactaaa gggaacaaaa gctggagctg caagc
//
