LOCUS       sequence-375065-       13339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13339)
  AUTHORS   Harpavat S, Cepko CL
  TITLE     RCAS-RNAi: a loss-of-function method for the developing chick 
            retina.
  JOURNAL   BMC Dev Biol. 2006 Jan 22;6:2. doi: 10.1186/1471-213X-6-2.
  PUBMED    16426460
REFERENCE   2  (bases 1 to 13339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2006-01-22"; volume: "6:2. doi"; pages: " 
            10.1186/1471-213X-6-2"
COMMENT     Sequence Label: G-RCAS (A)
FEATURES             Location/Qualifiers
     source          1..13339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        434..695
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     protein_bind    7697..7821
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7846..7851,7852..7869,7870..7876)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7930..8589
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7996..8015)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8931..9236
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9115..9134
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(9277..9401)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        9542..9803
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(10393..10415)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10404..10420
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10428..10434,10435..10452,10453..10458))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(10501..10519)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10587..10691
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10692..10760,10761..11552)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10910..10929)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11723..12311
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12212..12231
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12465..12482
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12497..12637
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12563..12582)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12723..12745
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(12739..12930)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc
       61 caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg
      121 gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct
      181 ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg
      241 tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcga
      301 tgcgatgtac gggccagata tacgcgtatc tgaggggact agggtgtgtt taggcgaaaa
      361 gcggggcttc ggttgtacgc ggttaggagt cccctcagga tatagtagtt tcgcttttgc
      421 atagggaggg ggaaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
      481 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
      541 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
      601 gaaccactga attccgcatt gcagagatat tgtatttaag tgcctagctc gatacaataa
      661 acgccatttg accattcacc acattggtgt gcacctgggt tgatggccgg accgttgatt
      721 ccctgacgac tacgagcacc tgcatgaagc agaaggcttc atttggtgac cccgacgtga
      781 tagttaggga atagtggtcg gccacagacg gcgtggcgat cctgtctcca tccgtctcgt
      841 ctatcgggag gcgagttcga tgaccctggt ggagggggct gcggcttagg gaggcagaag
      901 ctgagtaccg tcggagggag ctccagggcc cggagcgact gacccctgcc gagaactcag
      961 agggtcgtcg gaagacggag agtgagcccg acgaccaccc caggcacgtc tttggtcggc
     1021 ctgcggatca agcatggaag ccgtcattaa ggtgatttcg tccgcgtgta aaacctattg
     1081 cgggaaaatc tctccttcta agaaggaaat aggggccatg ttgtccctgt tacaaaagga
     1141 agggttgctt atgtctccct cagatttata ttctccgggg tcctgggatc ccatcactgc
     1201 ggcgctctcc cagcgggcaa tggtacttgg aaaatcggga gagttaaaaa cctggggatt
     1261 ggttttgggg gcattgaagg cggctcgaga ggaacaggtt acatctgagc aagcaaagtt
     1321 ttggttggga ttagggggag ggagggtctc tcccccaggt ccggagtgca tcgagaaacc
     1381 agctacggag cggcgaatcg acaaagggga ggaggtggga gaaacaactg tgcagcgaga
     1441 tgcgaagatg gcgccagagg aagcggccac acctaaaacc gttggcacat cctgctatca
     1501 ttgcggaaca gctgttggct gcaattgcgc caccgccaca gcctcggccc ctcctccccc
     1561 ttatgtgggg agtggtttgt atccttccct ggcgggggtg ggagagcagc agggccaggg
     1621 agataacacg tctcgggggg cggagcagcc aagggaggag ccagggcacg cgggtcaggc
     1681 ccctgggccg gccctgactg actgggcaag ggtaagggag gagcttgcga gtactggtcc
     1741 gcccgtggtg gccatgcctg tagtgattaa gacagaggga cccgcctgga cccctctgga
     1801 gccaaaattg atcacaagac tggctgatac ggtcaggacc aagggcttac gatccccgat
     1861 cactatggca gaagtggaag cgctcatgtc ctccccgttg ctgccgcatg acgtcacgaa
     1921 tctaatgaga gtgattttag gacctgcccc atatgcctta tggatggacg cttggggagt
     1981 ccaactccag acggttatag cggcagccac tcgcgacccc cgacacccag cgaacggtca
     2041 agggcggggg gaacggacta acttggatcg attaaagggc ttagctgatg ggatggtggg
     2101 caacccacag ggtcaggccg cattattaag accgggggaa ttggttgcta ttacggcgtc
     2161 ggctctccag gcgtttagag aagttgcccg gctggcggaa cctgcaggtc catgggcgga
     2221 catcacgcag ggaccatctg agtcctttgt tgattttgcc aatcggctta taaaggcggt
     2281 tgaggggtca gatctcccgc cttccgcgcg ggctccggtg atcattgact gctttaggca
     2341 gaagtcacag ccagatattc agcagcttat acgggcagca ccctccacgc tgaccacccc
     2401 aggagagata atcaaatatg tgctagacag gcagaagatt gcccctctta cggatcaagg
     2461 catagccgcg gccatgtcgt ctgctatcca gcccttagtt atggcagtag tcaatagaga
     2521 gagggatgga caaactgggt cgggtggtcg tgcccgaggg ctctgctaca cttgtggatc
     2581 cccgggacat tatcaggcac agtgcccgaa aaaacgaaag tcaggaaaca gccgtgagcg
     2641 atgtcagctg tgtgacggga tgggacacaa cgctaaacag tgtaggaagc gggatggcaa
     2701 ccagggccaa cgcccaggaa gaggtctctc ttcggggccg tggcccggcc ctgagcagcc
     2761 tgccgtctcg ttagcgatga caatggaaca taaagatcgc cccttggtta gggtcattct
     2821 gactaacact gggagtcatc cagtcaaaca acgttcggtg tatatcaccg cgctgttgga
     2881 ctccggagcg gacatcacta ttatttcgga ggaggattgg cctactgatt ggccggtggt
     2941 ggacaccgcg aacccacaga tccatggcat aggaggggga attcccatgc gaaaatcccg
     3001 ggatatgata gaggtggggg ttattaaccg agacgggtcg ttggagcgac ccctgctcct
     3061 cttccccgca gtcgctatgg ttagagggag tatcctagga agagattgtc tgcagggcct
     3121 agggctccgc ttgacaaatt tatagggagg gccactgttc ttactgttgc gctacatctg
     3181 gctattccgc tcaaatggaa gtcagaccgc acgcctgtgt ggattgacca gtggcccctt
     3241 cctgaaggta aacttgtagc gctaacgcaa ttagtggaaa aagaattaca gttaggacat
     3301 atagagccct cacttagttg ttggaacaca cctgtttttg tgatccggaa ggcttccggg
     3361 tcttatcgct tattgcatga tttgcgcgct gttaacgcca agcttgttcc ttttggggcc
     3421 gtccaacagg gggcgccagt tctctccgcg ctcccgcgtg gctggcccct gatggtccta
     3481 gacctcaagg attgcttctt ttctatccct cttgcggaac aagatcgcga agcttttgca
     3541 tttacgctcc cctctgtgaa taaccaggcc cccgctcgaa gattccaatg gaaggtcttg
     3601 ccccaaggga tgacctgttc tcccactatc tgtcagttgg tagtgggtca ggtgctcgag
     3661 cccttgcgac tcaagcaccc agctctgcgc atgttgcatt atatggacga tcttttgcta
     3721 gccgcctcaa gtcatgatgg gttggaagcg gcagggaagg aggttatcgg tacattggaa
     3781 agagccgggt tcactatttc gccggataag atccagaggg agcccggagt acaatatctt
     3841 gggtacaagt taggcagtac gtatgtagca cccgtaggct tggtagcaga acccaggata
     3901 gccaccttgt gggatgttca aaagctggtg gggtcacttc agtggcttcg cccagcgtta
     3961 gggatcccgc cacgactgat gggtcccttt tatgagcagt tacgagggtc agatcctaac
     4021 gaggcgaggg aatggaatct agacatgaaa atggcctgga gagagatcgt acagcttagc
     4081 actactgctg ccttggaacg atgggaccct gcccagcctc tggaaggagc ggtcgctaga
     4141 tgtgaacagg gggcaatagg ggtcctggga cagggactgt ccacacaccc aaggccatgt
     4201 ttgtggttat tctccaccca acccaccaag gcgtttactg cttggttaga agtgctcacc
     4261 cttttgatta ctaagctacg cgcttcggca gtgcgaacct ttggcaagga ggttgatatc
     4321 ctcctgttgc ctgcatgctt ccgggaggac cttccgctcc cggaggggat cctgttagca
     4381 cttagggggt ttgcaggaaa aatcaggagt agtgacacgc catctatttt tgacattgcg
     4441 cgtccactgc atgtttctct gaaagtgagg gttaccgacc accctgtgcc gggacccact
     4501 gtctttaccg acgcctcctc aagcacccat aaaggggtgg tagtctggag ggagggccca
     4561 aggtgggaga taaaagaaat agttgatttg ggggcaagtg tacaacaact ggaggcacgc
     4621 gctgtggcca tggcacttct gctgtggccg acaacgccca ctaatgtagt gactgactct
     4681 gcgtttgttg cgaaaatgtt actcaagatg ggacaggagg gagtcccgtc tacagcggca
     4741 gcttttattt tagaggatgc gttaagccaa aggtcagcca tggccgccgt tctccacgtg
     4801 cggagtcatt ctgaagtgcc agggtttttc acagaaggaa atgacgtggc agatagccaa
     4861 gccacctttc aagcgtatcc cttgagagag gctaaagatc ttcataccgc tctccatatt
     4921 ggaccccgcg cgctatccaa agcgtgtaat atatctatgc agcaggctag ggaggttgtt
     4981 cagacctgcc cgcattgtaa ttcagcccct gcgttggagg ccggggtaaa ccctaggggt
     5041 ttgggacccc tacagatatg gcagacagac tttacgcttg agcctagaat ggctccccgt
     5101 tcctggctcg ctgttactgt ggacaccgcc tcatcagcga tagtcgtaac tcagcatggc
     5161 cgtgttacat cggttgctgc acaacatcat tgggccacgg ctatcgccgt tttgggaaga
     5221 ccaaaggcca taaaaacaga taacgggtcc tgcttcacgt ctaaatccac gcgggagtgg
     5281 ctcgcgagat gggggatagc acacaccacc gggattccgg gaaattccca gggtcaagct
     5341 atggtagagc gggccaaccg gctcctgaaa gataagatcc gtgtgcttgc ggaaggggac
     5401 ggctttatga aaagaatccc cgccagcaaa cagggggaac tactagccaa agcaatgtat
     5461 gccctcaatc actttgagcg tggtgaaaac acaaaaacac cggtacaaaa acactggaga
     5521 cctaccgttc ttacagaagg acccccggtt aaaatacgaa tagagacagg ggagtgggaa
     5581 aaaggatgga acgtgctggt ctggggacga ggttatgccg ctgtgaaaaa cagggacact
     5641 gataaggtta tttgggtacc ctctcggaaa gttaaaccgg atgtcaccca aaaggatgag
     5701 gtgactaaga aagatgaggc gagccctctt tttgcaggca tttctgactg gataccctgg
     5761 gaagacgagc aagaaggact ccaaggagaa accgctagca acaagcaaga aagacccgga
     5821 gaagacaccc ttgctgccaa cgagagttaa ttatattctc attattggtg tcctggtctt
     5881 gtgtgaggtt acgggggtaa gagctgatgt ccacttactc gagcagccag ggaacctttg
     5941 gattacatgg gccaaccgta caggccaaac ggatttttgc ctctctacac agtcagccac
     6001 ctcccctttt caaacatgtt tgataggtat cccgtcccct atttccgagg gtgattttaa
     6061 gggatatgtt tctgatacaa attgcaccac cttgggaact gatcggttag tctcgtcagc
     6121 cgactttact ggcggacctg acaacagtac caccctcact tatcggaagg tctcatgctt
     6181 gttgttaaag ctgaatgtct ctatgtggga tgagccacct gaactacagc tgttaggttc
     6241 ccagtctctc cctaacatta ctaatattgc tcagatttcc ggtataaccg ggggatgcgt
     6301 aggcttcaga ccacaagggg ttccttggta tctaggttgg tctagacagg aggccacgcg
     6361 gtttctcctt agacacccct ctttctctaa atccacggaa ccgtttacgg tggtgacagc
     6421 ggataggcac aatcttttta tggggagtga gtactgcggt gcatatggct acagattttg
     6481 gaacatgtat aactgctcac aggtggggcg gcagtaccgc tgtggtaatg cgcgcagccc
     6541 ccgcccgggt cttcctgaaa tccagtgtac aaggagagga ggcaaatggg ttaatcaatc
     6601 acaggaaatt aatgagtcgg agccgttcag ctttacggtg aactgtacag ctagtagttt
     6661 gggtaatgcc agtgggtgtt gcggaaaagc aggcacgatt ctcccgggaa agtgggtcga
     6721 cagcacacaa ggtagtttca ccaaaccaaa agcgctacca cccgcaattt tcctcatttg
     6781 tggggatcgc gcatggcaag gaattcccag tcgtccggta gggggcccct gctatttagg
     6841 caagcttacc atgttagcac ctaagcatac agatattctc aaggtgcttg tcaattcatc
     6901 gcggacaggt ataagacgta aacgaagcac ctcacacctg gatgatacat gctcagatga
     6961 agtgcagctt tggggtccta cagcaagaat ctttgcatct atcctagccc cgggggtagc
     7021 agctgcgcaa gccttaagag aaattgagag actagcctgt tggtccgtta aacaggctaa
     7081 cttgacaaca tcactcctcg gggacttatt ggatgatgtc acgagtattc gacacgcggt
     7141 cctgcagaac cgagcggcta ttgacttctt gctcctagct cacggccatg gctgtgagga
     7201 cgttgccgga atgtgctgtt tcaatttgag tgatcacagt gagtctatac agaagaagtt
     7261 ccagctaatg aaggaacatg tcaataagat cggcgtggac agcgacccaa ttggaagttg
     7321 gctgcgagga ctattcgggg gaataggaga atgggccgtt catttgctga aaggactgct
     7381 tttggggctt gtagttattt tgttgctagt agtgtgcctg ccttgccttt tgcaaatcgt
     7441 atgcggtaac atcagaaaga tgattaataa ctccatcagc taccacacgg aatataagaa
     7501 gctgcaaaag gcctgtgggc agcctgaaag cagaatagta taaggcagta catgggtggt
     7561 ggtatagcgc ttgcgagtcc atcgaggaag gcaggaaaga cagctattgg taattgtgaa
     7621 atacgctttt gtctgtgtgc tgcaggagct gagctgactc tgctggtggc ctcgcgtacc
     7681 actgtggcat cgatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
     7741 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     7801 acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct
     7861 tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag
     7921 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
     7981 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
     8041 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
     8101 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
     8161 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
     8221 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
     8281 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     8341 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
     8401 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
     8461 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
     8521 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
     8581 ggggcgtaaa gatctggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc
     8641 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga
     8701 ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca
     8761 aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg
     8821 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg
     8881 gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta
     8941 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
     9001 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
     9061 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
     9121 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca
     9181 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
     9241 caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt
     9301 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
     9361 tcattttacg tttctcgttc agctttcttg tacaaagtgg ttgatcgatg cgatgtacgg
     9421 gccagatata cgcgtatctg aggggactag ggtgtgttta ggcgaaaagc ggggcttcgg
     9481 ttgtacgcgg ttaggagtcc cctcaggata tagtagtttc gcttttgcat agggaggggg
     9541 aaatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     9601 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     9661 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     9721 tccgcattgc agagatattg tatttaagtg cctagctcga tacaataaac gccatttgac
     9781 cattcaccac attggtgtgc acctgggttg atggccggac cgttgattcc ctgacgacta
     9841 cgagcacctg catgaagcag aaggcttcat ttggtgaccc cgacgtgata gttagggaat
     9901 agtggtcggc cacagacggc gtggcgatcc tgtctccatc cgtctcgtct atcgggaggc
     9961 gagttcgatg accctggtgg agggggctgc ggcttaggga ggcagaagct gagtaccgtc
    10021 ggaggggatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag
    10081 tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg
    10141 tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat
    10201 gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat
    10261 gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat
    10321 acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagctccaa
    10381 gcttggctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacat tatacgagcc
    10441 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gaattcttga agacgaaagg
    10501 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
    10561 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    10621 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    10681 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    10741 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
    10801 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    10861 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    10921 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    10981 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    11041 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    11101 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    11161 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    11221 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    11281 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    11341 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    11401 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    11461 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    11521 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    11581 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    11641 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    11701 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    11761 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    11821 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
    11881 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    11941 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    12001 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    12061 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    12121 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    12181 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    12241 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    12301 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    12361 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    12421 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    12481 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
    12541 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
    12601 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
    12661 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
    12721 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct
    12781 gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
    12841 gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat
    12901 gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaaggag gatttctgtt
    12961 catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
    13021 tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
    13081 ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt
    13141 tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc
    13201 tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc
    13261 tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
    13321 attctgctaa ccagtaagg
//
