LOCUS       sequence-375101-        5526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry plasmid (attL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Liu S, Sretenovic S, Fan T, Cheng Y, Li G, Qi A, Tang X, Xu Y, Guo 
            W, Zhong Z, He Y, Liang Y, Han Q, Zheng X, Gu X, Qi Y, Zhang Y
  TITLE     Hypercompact CRISPR-Cas12j2 (CasPhi) enables genome editing, gene 
            activation, and epigenome editing in plants.
  JOURNAL   Plant Commun. 2022 Sep 20:100453. doi: 10.1016/j.xplc.2022.100453.
  PUBMED    36127876
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2022 Sep 20:100453. doi: 10.1016/j.xplc.2022.100453."
COMMENT     Sequence Label: pYPQCas12j2
FEATURES             Location/Qualifiers
     source          1..5526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             360..1151
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1244..1832
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1733..1752
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1986..2003
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2162..2189)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2281..2367)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2416..2438
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2430..2447
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2431..2447
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2463..2562
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2587..2607
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4900..4947
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      4978..5230
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    5275..5398
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5415..5434)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5416..5434)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5439..5455)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5439..5455)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tctggaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac
       61 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
      121 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
      181 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
      241 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
      301 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta
      361 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
      421 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
      481 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
      541 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
      601 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
      661 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
      721 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
      781 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
      841 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
      901 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
      961 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     1021 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     1081 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     1141 tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt
     1201 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1261 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1321 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1381 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1441 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1501 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1561 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1621 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1681 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1741 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1801 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     1861 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     1921 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     1981 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     2041 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     2101 gcagtgagcg caacgcaatt aatacgcgta ccgcgagcca ggaagagttt gtagaaacgc
     2161 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
     2221 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
     2281 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     2341 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
     2401 ttgcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
     2461 cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc
     2521 aatgcttttt tataatgcca actttgtaca aaaaagcagg ctccgaattc gcccttcacc
     2581 atggctccta agaagaagcg gaaggttggt attcacgggg tgcctgcggc tcctaaaccg
     2641 gctgtcgaaa gcgaattctc taaagtgttg aaaaagcact tccctggcga gagattcaga
     2701 tcatcctaca tgaaaagagg ggggaagatt ctggcggctc aaggagagga agccgtcgtg
     2761 gcatatctcc agggcaaatc cgaggaggaa ccaccgaact ttcaacctcc agcaaagtgc
     2821 catgtggtca cgaagtcccg ggattttgcg gagtggccaa tcatgaaggc aagtgaagcc
     2881 atacagcgct atatctacgc ccttagcact actgaaagag cggcgtgtaa gcctggaaaa
     2941 tcctctgagt cgcacgcggc atggtttgct gctacagggg tgtctaatca tggatatagc
     3001 catgttcagg gtcttaacct gatctttgac catacactgg gacgctacga tggggtgttg
     3061 aagaaggttc aactgaggaa tgaaaaagca agagcgcgcc ttgagagcat caacgcgagc
     3121 cgcgctgacg aaggactccc ggaaatcaag gcagaagaag aagaagttgc aacgaatgag
     3181 acgggacact tgctgcagcc acccggaata aacccttcct tttatgttta tcagactatc
     3241 agccctcagg cttatcgccc ccgcgatgag atcgtccttc cgcctgagta cgctggctac
     3301 gttagggacc caaacgcgcc tataccgttg ggcgtcgtta ggaatagatg cgatatacag
     3361 aaaggctgcc cagggtatat acccgagtgg cagcgcgagg ctgggactgc gatatcacct
     3421 aaaaccggca aagccgttac ggtgccggga ttgagtccta aaaaaaataa gagaatgagg
     3481 aggtattggc ggtcagagaa ggagaaggcc caagatgcac tccttgtcac cgtgaggata
     3541 ggcacggatt gggtcgtcat tgacgttaga gggcttttga ggaacgcacg gtggaggact
     3601 attgcgccga aggacattag tctgaacgcg cttctcgatc tgttcactgg cgacccagtt
     3661 attgatgtga ggcggaacat agtcaccttt acttatactc tggacgcatg cggaacatac
     3721 gctcgcaagt ggaccctgaa aggaaagcag actaaggcca cactggataa gttgacagcc
     3781 acgcagacag tcgctttggt ggccatagac cttgggcaaa ccaacccaat tagtgccggg
     3841 atcagcaggg ttactcagga gaatggtgca cttcagtgcg agcctctcga tcgctttaca
     3901 ctccccgatg atcttcttaa agacatcagt gcctacagaa tcgcctggga ccggaacgag
     3961 gaggaactcc gcgctcgcag tgttgaagcc ttgccagaag cacagcaggc tgaggttagg
     4021 gcactggatg gcgtttctaa agagactgcc agaactcaat tgtgtgctga ttttgggctt
     4081 gatcctaaga gactcccttg ggataaaatg tcgtcgaata ctaccttcat ctccgaggcc
     4141 ctgcttagta acagcgttag tcgcgaccaa gtctttttca cacccgcccc gaaaaaaggc
     4201 gcgaaaaaga aagctccggt ggaagttatg aggaaagacc gcacatgggc acgcgcttac
     4261 aaaccgagac tgtcagttga agcgcagaaa ttgaagaacg aagcactttg ggccttgaag
     4321 cggacatctc cagagtatct taaactctcc aggagaaagg aagaactctg tagaaggtcg
     4381 attaattacg ttatagagaa gacacgccgg aggactcagt gtcaaattgt tattccggtc
     4441 atcgaagacc tcaatgttcg cttctttcat ggttctggaa agcggttgcc cggatgggac
     4501 aatttcttta cagctaaaaa agaaaaccgc tggttcatac aaggattgca caaggccttt
     4561 agtgatctcc ggacgcatcg ctccttttat gtcttcgagg tgagaccgga aagaacgtcc
     4621 atcacatgcc caaagtgtgg acattgtgaa gtcgggaacc gggacggtga ggcctttcaa
     4681 tgcctttcgt gcgggaagac ctgtaatgcg gatcttgatg tggctacgca taatctcact
     4741 caggtggctc tcacaggcaa aacaatgccg aagagagagg aacctaggga tgcacaaggg
     4801 acggccccgg ccagaaaaac gaagaaggct agcaagtcca aggcgccgcc ggcggagagg
     4861 gaggatcaga ctccagccca ggaaccatcc caaacaagta agcgtcctgc tgccaccaaa
     4921 aaggccggac aggctaagaa aaagaagtga gacgactagt ggcggccgcc gacgtccgat
     4981 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     5041 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     5101 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     5161 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     5221 ttactagatc gggaattgat cccccctcga cagcttccgg aaagggcgaa ttcgcaactt
     5281 tgtatacaaa agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
     5341 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatgcc
     5401 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     5461 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     5521 gcctcc
//
