LOCUS       sequence-37513-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Liu C, Xu P, Lamouille S, Xu J, Derynck R
  TITLE     TACE-mediated ectodomain shedding of the type I TGF-beta receptor 
            downregulates TGF-beta signaling.
  JOURNAL   Mol Cell. 2009 Jul 10. 35(1):26-36.
  PUBMED    19595713
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Jul 10. 35(1):26-36."
COMMENT     Sequence Label: pRK5M-TIMP3
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(45..74)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(787..805)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(788..805)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 attaagctgc ataaacagtt gggccatggc ggccaagctt acagcaggtc ctcctcgctg
       61 atcagcttct gctcgtcgac ggggtctgtg gcattgatga tgcttttatc cgggggggcc
      121 catcctcggt accagctgca gtagccgccc ttctgccgga tgcaggcgta gtgtttggac
      181 tggtagccag ggtaaccgaa attggagagc atgtcggtcc agagacactc gttcttggaa
      241 gtcacaaagc aaggcaggta gtagcaggac ttgatcttgc agttacaacc caggtgatac
      301 cgatagttca gccccttgcg ctgggagagg gtgagctggt cccacctctc cacgaagttg
      361 cacagccccg tgtacatctt gccatcatag acgcgacctg tcagcaggta ctggtacttg
      421 ttgacctcca gcttaaggcc acagagactc tcggaagctt ccgtgtggat gtactgcaca
      481 tggggcatct tggtgaagcc tcggtacatc ttcatctgct tgatggtgta gaccagcgtg
      541 ccgaagggcc cctcctttac cagcttcttc cccaccacct tggcccggat cacgatgtcg
      601 gagttgcaga aggcgtcctg ggggtggctg ggcgagcatg tgcacgcctc ggcgccccag
      661 tcccccaggc tccagctgcc caggagcacg atgagcccga gccaaggggt cattgcatcg
      721 atagaaccga ggtgcagttg gacctgggag tggacacctg tggagagaag gcaaagtgga
      781 tgttattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt
      841 gtagccgcgt tctaacgaa
//
