LOCUS       sequence-375147-       22142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multi-host shuttle plasmid for D. radiodurans, E. coli, S. 
            cerevisiae, S. meliloti, and P. tricornutum; contains an oriT for 
            transfer via conjugation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22142)
  AUTHORS   Brumwell SL, Van Belois KD, Giguere DJ, Edgell DR, Karas BJ
  TITLE     Conjugation-Based Genome Engineering in Deinococcus radiodurans.
  JOURNAL   ACS Synth Biol. 2022 Mar 18;11(3):1068-1076. doi: 
            10.1021/acssynbio.1c00524. Epub 2022 Mar 7.
  PUBMED    35254818
REFERENCE   2  (bases 1 to 22142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00524"; journalName: "ACS Synth Biol"; date: 
            "2022-03-18- 18"; volume: "11"; issue: "3"; pages: "1068-1076"
COMMENT     Sequence Label: pDEINO1
FEATURES             Location/Qualifiers
     source          1..22142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             600..1169
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFT
                     LREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRR
                     LTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFTL
                     CGLDTALYDGTASDGEQALYMSMPCP"
     rep_origin      5904..6512
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      5995..6520
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      6588..6807
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             6898..7653
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    7656..7906
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     misc_feature    8068..8571
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        8572..8758
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             8759..9421
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     CDS             9611..10786
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             10786..11757
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    11830..12303
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    12563..12961
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(12979..13012)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     13080..13099
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(13179..13454)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGETIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL
                     AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS"
     primer_bind     13292..13314
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13306..13323
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13307..13323
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13330..13349
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13330..13348
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    13351..13407
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(13450..13466)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13450..13466)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13463..13485)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13474..13490
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13498..13504,13505..13522,13523..13528))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13543..13564
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(13784..14443)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     14358..14377
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(14444..14546)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(15581..16375)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(15692..15711)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     16302..16321
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     oriT            21507..21616
                     /note="incP origin of transfer"
     CDS             21649..22020
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 atcgatacta gcttgattgg gatatctcgc tcgtgcttgt cgcgtgctat gtcttttagg
       61 gtactttgaa cctacgttcg tacttgtata atatgatcat cgtattatcg tttttcatcc
      121 gtccagcgca aaatgcatta gcagctagtc ctagcgtgcg gagctacctg gacaggtgca
      181 tgacggatgc gtgtccttca gtgactttct aattaacagt aacttcttta cttatgtttc
      241 agtttgtaag aagcgggatt cgctcgtcgg ttgacatctg attggactgc gtcggcacat
      301 gaaaactaca ttgtgaaatc tgctaaaact ccgggtatct ctgacacaaa acgattcggc
      361 ttcgcaattt caacattacg gtcaaggcta acgtatcttt ctcggtcaac ttcagattat
      421 gccgattaaa ttgtcgtagc tttcaaggcg ttttgagtac tgcggcagtt gttgaacctg
      481 caaggagaag atctcgacaa cagaataaag cgaaaaatgg gtctcatgca ctaacactca
      541 ggcctccctc ataatctctg tttgagttta ccaacaacac atatatacat ttcgacaaaa
      601 tgaccactct tgacgacacg gcttaccggt accgcaccag tgtcccgggg gacgccgagg
      661 ccatcgaggc actggatggg tccttcacca ccgacaccgt cttccgcgtc accgccaccg
      721 gggacggctt caccctgcgg gaggtgccgg tggacccgcc cctgaccaag gtgttccccg
      781 acgacgaatc ggacgacgaa tcggacgacg gggaggacgg cgacccggac tcccggacgt
      841 tcgtcgcgta cggggacgac ggcgacctgg cgggcttcgt ggtcgtctcg tactccggct
      901 ggaaccgccg gctgaccgtc gaggacatcg aggtcgcccc ggagcaccgg gggcacgggg
      961 tcgggcgcgc gttgatgggg ctcgcgacgg agttcgcccg cgagcggggc gccgggcacc
     1021 tctggctgga ggtcaccaac gtcaacgcac cggcgatcca cgcgtaccgg cggatggggt
     1081 tcaccctctg cggcctggac accgccctgt acgacggcac cgcctcggac ggcgagcagg
     1141 cgctctacat gagcatgccc tgcccctgat tttgttacat ttactgactt caaggagtcg
     1201 aggaatcgat actgccgtcg tttccaggat ccgaggtttc ataaactctg ttaacgttat
     1261 agaaacagac ttacctctcc tacgccattc acgtaatatt cgcaatatgc tattcttcct
     1321 ctgaagacca ggtttatgtg ctgcctgaaa ctatttcaat aagtcagctg cacttgcaca
     1381 gggtttcaca aggaaagcgt gtcttttttt ccaacgtagg cgtcgctttc gtctgactct
     1441 tactcttaca ttcacagcca atacttacaa ttagtaaaaa acctgtgctc gagagtgaaa
     1501 actccgtaac tataacggtc ctaaggtagc gaacactccg ttcgtggcgt cgcacgatcg
     1561 cgacatttga cgacgtgtgt tttgcctggg ctacgtatcg ggaatgtttg cgaaaccccc
     1621 gaaattgtac cgggatgtag gcacatcgct ggaaggtagc caggggggtc cggcgctgat
     1681 tacgaagtgc ggcgaccgac gaatttggag cacgcccgac gctcaggggt ggatgccgct
     1741 ggcgattccg cttgagtggg atcgctcggc acccgcgggt cggcatatgc ccgtgccgat
     1801 tgtgtcccca cggtgttggt caggcggccg gtatcacgac accccccgcc catcgaagcc
     1861 attcgctctc agcaacgcca tgagcatcgg gccaagcgag aatttccctt gccgcgcctt
     1921 ggcggtcaga tcgcgcagat agccgccagc agagttgata tgccctgccc tctctagaat
     1981 gcaggccacg gcgactccgg cgttttccgg tcccatgacc tcgcaggcct cctgataggc
     2041 cgaggggtaa acgccaagca tagatcgtac cgtgactgca gcgatcatga ggtcgcgcca
     2101 acttctcact gcaccgtcag gaccgtagtt catgatttcc gggcaggctt tcagtaccat
     2161 tcccagcgga aaggcccttt ttgccttgtt ggccggttca atggcttggc cgtctaaccg
     2221 ctcattccga gagcaacgtt caagttcaat aatagattcg ggttttgatt cctgtttgtg
     2281 ccgctcagtt tgatcgggat tgccgctcat ctcgtgtggt tttatgtgct tttgcaactg
     2341 gttagccacc tgttcgcgca gcgtttcgaa tgccactacc aaaggggtga gatcagcgat
     2401 atcgggcgtc ctcggtatcg cctggacgag gcttcggaaa tcttcctgca tagacgtcca
     2461 gtggcccggc acttctacga gcaccacgtc tatcaacttg gcgatgtctc tgcggcagag
     2521 cgtcagtcgc tcgcgtagcc gctgcagctc catccgatca gcctcggctt cggcagcgag
     2581 tcgctctatt tcgccggagc gggccagcag tggtgcgagc gaaaagccga acgctgtgcc
     2641 gatcgctcca tcgcgagtct tgcgtgcata gcgcttgccg ttcggactgt ccttgcgagc
     2701 gaccagccct gcatcgacga gggcggccag atgccttctg agcgtagcct cggccatgcc
     2761 atgagctctc agtgacagct gaacgttcga gggaaagacg accagactgg tctccccgct
     2821 tagcgtgttc tccggataga agctcaacag cgcgttcaga acggctagtg tccgatcgct
     2881 gatgccaaga cggcgtttgg cagcacaaag cgagcgaaac aatttccact tgtcgaccga
     2941 cttgccaggt tcgatgtcgc gggagatgaa ctggcctgcc aacagggcaa gcgtcatcga
     3001 ccgccgccca aagggcgtcg tcacacttcc aatctccatt tccttcacct tcgataaggc
     3061 aaaagaaagc agctcaccaa aaacggtgcc aaggactctt gactgtgatt cgtggaaatg
     3121 cgattctcga tcttgctaca aatctgagga aggcttccac ggcttgttcg ttgggggcct
     3181 ttttcttttg cgggttagtc tccttgtttc gaactctgct tttgcatgaa ctggtcgtgc
     3241 aggctttcga tctgacctgc aatgaaatcg acaaagcctg acggcacaga cttgttgaat
     3301 gtcagcttcg cagccgactt gccgcgttgg atctggccaa gcacgaggcc atgagagatc
     3361 agatcgctcg tggaagacgc tgcagccctc acatcaacct tggtaagggc ttcaaaaacc
     3421 gcagtgaagc gtagatcgct ggaagaggct tgaatctgct cttgctccag aaccaactgc
     3481 gccaccttcg cgggctcccg gtgagccgcc cagcgcactt cgaactcgat ccatctacgc
     3541 cgtccgattt caggagccgg accaatctgc atcaggatct cgcgcggcaa tgctgccgct
     3601 atccggatca tttcggagat atgcgacctg ccggtgctca gggacttgca gatcacgtcg
     3661 cggcgatagc cggcagcctc ctgcgattgc gcaaaaaggc atcgctcgat gaaggacagg
     3721 tccttgcggt tgctgttctc ttccccttgg agaacgatcg catcttcgtc gctaagttcc
     3781 cgggcgattg ccttgaactt gatgccgagg agtttggcag cggcaagccg gcgccggcca
     3841 aatacgatct gaaacggcct tgcggcccct cgaaccgggc ggaccaagcc aggagtactc
     3901 tgtccatgct cgcgcatcga ctcgagcaaa tccgcaatac cggcttcgct gtagccggaa
     3961 tcaaagcgat cccggatggc cgattccatg atctcgtctg gatcgatctc gacgatctgg
     4021 ccaccatcct tgagaaggcg ttcggcaagt tcgctgcgtt cctgcatctg acggacgccg
     4081 gctgcaactt tgagaagatg tggcgatgtc gaccgctgca tcccgccctt ctctgcagaa
     4141 ttttccagct gtccgaggac attggcaaaa aggcccttgg aatccttgcg gctcattctc
     4201 tcccccatgc cttccagaag aggccctcga gttcagcatt ggcagcattg atcgactcga
     4261 tggcgcggtc gtaggttttc ggcgccgaaa atcgggacct ctgaacctcg taaaggctct
     4321 gcttccacgt cagagcatcg gccaccgcgg tcgacttgat gacggagttg aggagcagat
     4381 cctcgccgaa cacctgtctc atcagcgcgg acatattcgc ttgcggatga tcgttcggct
     4441 cgaactttgt gatcaggaac ttggcgaagt cccattgagc ggcggtgccc acctcattca
     4501 ggatctgcat gtacgacccg gcaagctcca aaaactttgc ggtcgagtcc acatccagca
     4561 tatgtgccgg aaccgggatg atgactccag tcgcggcggt cagcgacgaa agtgtcagga
     4621 agttcagcga cggagccgag tccataagga cgaagtcgta gtcatcggcg acctgctcca
     4681 gagcttgaga aacgcgaagc aagaaaccgg ttcctgctga ccgccgcatc tcgagggcga
     4741 cggccgtctc gaactcggtc agttcgaggc cggcacagat gacgtcgaaa ccaacgatgt
     4801 gggtcttgtg gatgatggat ttcgtcggga cgggatcgtc gaaacgaatg gctgagaaaa
     4861 gtgtatcgcc gccgcggtaa tcgaattcgg gcaggctacc atggagcgcg gtgagagatg
     4921 cttgcgggtc gagatcgacc gccagcacgc gatagccctt gagtgccaga tagtgaccga
     4981 gatggattgt ggtagaggtc ttgctggagc cgcccttgaa gttggtgacc gcaacgatct
     5041 ggcattgctc attgcctgtc cggcgggggt tcatccattt cttccggccg tttgccgcaa
     5101 gagccatacg tagctcaaga acctgctcga gcgtgtagaa gcgccggccg ttggcgattt
     5161 cgggctcagg gccctgttcc ttggcggcaa cctggcggat ataggcctcg gttacgccca
     5221 gcagggtggc aacttcggta gaggtgaact tgcgcattgt gcgacgcgcg ttcgggggga
     5281 actgcgccat gctcaagttg ttcagtgcca tttcgagctt actggaaaaa ttctccatga
     5341 aagcgaggct gcttaggtgt cgcgtcatcg aaagaatccc tgtgatcgtt tccgtcactt
     5401 aagcagtgaa gtggttaacc gcttgctaat cgccacgtaa ctactcgaaa acacgcaaat
     5461 tcgaaaaatt tccgcaacta agacgtactc cgattcggct atggccatca atgcgcgctg
     5521 tggatgacgc acagagccgg ggggaaggcg ccagtgcatg ccggttctcg accctccccg
     5581 cctctttttt ggacgaagat gtgcgcagac gtcgctgggc ggagacgcaa tagtggttca
     5641 ctgcggaaat ggttggccgt tgctttcaat ggagatctgc cgaactcttc gcagagttcc
     5701 ccgcggggaa ctctcgaagc gacgacgctg gcgcgcagta ccgactgctc gccaaggcga
     5761 gacatatgtg ccgtattggg aacagctcgg catctcggtg ctcgcctgcg ctccggcctg
     5821 cctcaatctc gttggccatg accgagcaac gactgcaccg gatggttcag ttacgctagg
     5881 gataacaggg taatatagtc gagctggttg ccctcgccgc tgggctggcg gccgtctatg
     5941 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccggccgc
     6001 cggcgttgtg gatacctcgc ggaaaacttg gccctcactg acagatgagg ggcggacgtt
     6061 gacacttgag gggccgactc acccggcgcg gcgttgacag atgaggggca ggctcgattt
     6121 cggccggcga cgtggagctg gccagcctcg caaatcggcg aaaacgcctg attttacgcg
     6181 agtttcccac agatgatgtg gacaagcctg gggataagtg ccctgcggta ttgacacttg
     6241 aggggcgcga ctactgacag atgaggggcg cgatccttga cacttgaggg gcagagtgct
     6301 gacagaccgc acctattgac atttgagggg ctgtccacag gcagaaaatc cagcatttgc
     6361 aagggtttcc gcccgttttt cggccaccgc taacctgtct tttaacctgc ttttaaacca
     6421 atatttataa accttgtttt taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg
     6481 aaggggggtg cccccccttc tcgaaccctc ccggtcgagt gagcgaggaa gcaccaggga
     6541 acagcactta tatattctgc ttacacacga tgcctgaaaa aacttccctt ggggttatcc
     6601 acttatccac ggggatattt ttataattat tttttttata gtttttagat cttctttttt
     6661 agagcgcctt gtaggccttt atccatgctg gttctagaga aggtgttgtg acaaattgcc
     6721 ctttcagtgt gacaaatcac cctcaaatga cagtcctgtc tgtgacaaat tgcccttaac
     6781 cctgtgacaa attgccctca gaagaagctg ttttttcaca aagttatccc tgcttattga
     6841 ctctttttta tttagtgtga caatctaaaa acttgtcaca cttcacatgg atctgtcatg
     6901 gcggaaacag cggttatcaa tcacaagaaa cgtaaaaata gcccgcgaat cgtccagtca
     6961 aacgacctca ctgaggcggc atatagtctc tcccgggatc aaaaacgtat gctgtatctg
     7021 ttcgttgacc agatcagaaa atctgatggc accctacagg aacatgacgg tatctgcgag
     7081 atccatgttg ctaaatatgc tgaaatattc ggattgacct ctgcggaagc cagtaaggat
     7141 atacggcagg cattgaagag tttcgcgggg aaggaagtgg ttttttatcg ccctgaagag
     7201 gatgccggcg atgaaaaagg ctatgaatct tttccttggt ttatcaaacg tgcgcacagt
     7261 ccatccagag ggctttacag tgtacatatc aacccatatc tcattccctt ctttatcggg
     7321 ttacagaacc ggtttacgca gtttcggctt agtgaaacaa aagaaatcac caatccgtat
     7381 gccatgcgtt tatacgaatc cctgtgtcag tatcgtaagc cggatggctc aggcatcgtc
     7441 tctctgaaaa tcgactggat catagagcgt taccagctgc ctcaaagtta ccagcgtatg
     7501 cctgacttcc gccgccgctt cctgcaggtc tgtgttaatg agatcaacag cagaactcca
     7561 atgcgcctct catacattga gaaaaagaaa ggccgccaga cgactcatat cgtattttcc
     7621 ttccgcgata tcacttccat gacgacagga tagtctgagg gttatctgtc acagatttga
     7681 gggtggttcg tcacatttgt tctgacctac tgagggtaat ttgtcacagt tttgctgttt
     7741 ccttcagcct gcatggattt tctcatactt tttgaactgt aatttttaag gaagccaaat
     7801 ttgagggcag tttgtcacag ttgatttcct tctctttccc ttcgtcatgt gacctgatat
     7861 cgggggttag ttcgtcatca ttgatgaggg ttgattatca cagtttatta ctctgaattg
     7921 gctatccgcg tgtgtacctc tacctggagt ttttcccacg gtggatattt cttcttgcgc
     7981 tgagcgtaag agctatctga cagaacagtt cttctttgct tcctcgccag ttcgctcgct
     8041 atgctcggtt acacggctgc ggcgagcatc acgtgctata aaaataatta taatttaaat
     8101 tttttaatat aaatatataa attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat
     8161 agtttttgtt ttccgaagat gtaaaagact ctagggggat cgccaacaaa tactaccttt
     8221 taccttgctc ttcctgctct caggtattaa tgccgaattg tttcatcttg tctgtgtaga
     8281 agaccacaca cgaaaatcct gtgattttac attttactta tcgttaatcg aatgtatatc
     8341 tatttaatct gcttttcttg tctaataaat atatatgtaa agtacgcttt ttgttgaaat
     8401 tttttaaacc tttgtttatt tttttttctt cattccgtaa ctcttctacc ttctttattt
     8461 actttctaaa atccaaatac aaaacataaa aataaataaa cacagagtaa attcccaaat
     8521 tattccatca ttaaaagata cgaggcgcgt gtaagttaca ggcaagcgat cctagtacac
     8581 tctatatttt tttatgcctc ggtaatgatt ttcatttttt ttttccacct agcggatgac
     8641 tctttttttt tcttagcgat tggcattatc acataatgaa ttatacatta tataaagtaa
     8701 tgtgatttct tcgaagaata tactaaaaaa tgagcaggca agataaacga aggcaaagat
     8761 gacagagcag aaagccctag taaagcgtat tacaaatgaa accaagattc agattgcgat
     8821 ctctttaaag ggtggtcccc tagcgataga gcactcgatc ttcccagaaa aagaggcaga
     8881 agcagtagca gaacaggcca cacaatcgca agtgattaac gtccacacag gtatagggtt
     8941 tctggaccat atgatacatg ctctggccaa gcattccggc tggtcgctaa tcgttgagtg
     9001 cattggtgac ttacacatag acgaccatca caccactgaa gactgcggga ttgctctcgg
     9061 tcaagctttt aaagaggccc taggggccgt gcgtggagta aaaaggtttg gatcaggatt
     9121 tgcgcctttg gatgaggcac tttccagagc ggtggtagat ctttcgaaca ggccgtacgc
     9181 agttgtcgaa cttggtttgc aaagggagaa agtaggagat ctctcttgcg agatgatccc
     9241 gcattttctt gaaagctttg cagaggctag cagaattacc ctccacgttg attgtctgcg
     9301 aggcaagaat gatcatcacc gtagtgagag tgcgttcaag gctcttgcgg ttgccataag
     9361 agaagccacc tcgcccaatg gtaccaacga tgttccctcc accaaaggtg ttcttatgta
     9421 gttttacaca ggagtctgga cttgacgcta gtgataataa gtgactgagg tatgtgctct
     9481 tcttatctcc ttttgtagtg ttgctcttat tttaaacaac tttgcggttt tttgatgact
     9541 ttgcgatttt gttgttgctt tgcagtaaat tgcaagattt aataaaaaaa cgcaaagcaa
     9601 tgattaaagg atgttcagaa tgaaactcat ggaaacactt aaccagtgca taaacgctgg
     9661 tcatgaaatg acgaaggcta tcgccattgc acagtttaat gatgacagcc cggaagcgag
     9721 gaaaataacc cggcgctgga gaataggtga agcagcggat ttagttgggg tttcttctca
     9781 ggctatcaga gatgccgaga aagcagggcg actaccgcac ccggatatgg aaattcgagg
     9841 acgggttgag caacgtgttg gttatacaat tgaacaaatt aatcatatgc gtgatgtgtt
     9901 tggtacgcga ttgcgacgtg ctgaagacgt atttccaccg gtgatcgggg ttgctgccca
     9961 taaaggtggc gtttacaaaa cctcagtttc tgttcatctt gctcaggatc tggctctgaa
    10021 ggggctacgt gttttgctcg tggaaggtaa cgacccccag ggaacagcct caatgtatca
    10081 cggatgggta ccagatcttc atattcatgc agaagacact ctcctgcctt tctatcttgg
    10141 ggaaaaggac gatgtcactt atgcaataaa gcccacttgc tggccggggc ttgacattat
    10201 tccttcctgt ctggctctgc accgtattga aactgagtta atgggcaaat ttgatgaagg
    10261 taaactgccc accgatccac acctgatgct ccgactggcc attgaaactg ttgctcatga
    10321 ctatgatgtc atagttattg acagcgcgcc taacctgggt atcggcacga ttaatgtcgt
    10381 atgtgctgct gatgtgctga ttgttcccac gcctgctgag ttgtttgact acacctccgc
    10441 actgcagttt ttcgatatgc ttcgtgatct gctcaagaac gttgatctta aagggttcga
    10501 gcctgatgta cgtattttgc ttaccaaata cagcaatagc aatggctctc agtccccgtg
    10561 gatggaggag caaattcggg atgcctgggg aagcatggtt ctaaaaaatg ttgtacgtga
    10621 aacggatgaa gttggtaaag gtcagatccg gatgagaact gtttttgaac aggccattga
    10681 tcaacgctct tcaactggtg cctggagaaa tgctctttct atttgggaac ctgtctgcaa
    10741 tgaaattttc gatcgtctga ttaaaccacg ctgggagatt agataatgaa gcgtgcgcct
    10801 gttattccaa aacatacgct caatactcaa ccggttgaag atacttcgtt atcgacacca
    10861 gctgccccga tggtggattc gttaattgcg cgcgtaggag taatggctcg cggtaatgcc
    10921 attactttgc ctgtatgtgg tcgggatgtg aagtttactc ttgaagtgct ccggggtgat
    10981 agtgttgaga agacctctcg ggtatggtca ggtaatgaac gtgaccagga gctgcttact
    11041 gaggacgcac tggatgatct catcccttct tttctactga ctggtcaaca gacaccggcg
    11101 ttcggtcgaa gagtatctgg tgtcatagaa attgccgatg ggagtcgccg tcgtaaagct
    11161 gctgcactta ccgaaagtga ttatcgtgtt ctggttggcg agctggatga tgagcagatg
    11221 gctgcattat ccagattggg taacgattat cgcccaacaa gtgcttatga acgtggtcag
    11281 cgttatgcaa gccgattgca gaatgaattt gctggaaata tttctgcgct ggctgatgcg
    11341 gaaaatattt cacgtaagat tattacccgc tgtatcaaca ccgccaaatt gcctaaatca
    11401 gttgttgctc ttttttctca ccccggtgaa ctatctgccc ggtcaggtga tgcacttcaa
    11461 aaagccttta cagataaaga ggaattactt aagcagcagg catctaacct tcatgagcag
    11521 aaaaaagctg gggtgatatt tgaagctgaa gaagttatca ctcttttaac ttctgtgctt
    11581 aaaacgtcat ctgcatcaag aactagttta agctcacgac atcagtttgc tcctggagcg
    11641 acagtattgt ataagggcga taaaatggtg cttaacctgg acaggtctcg tgttccaact
    11701 gagtgtatag agaaaattga ggccattctt aaggaacttg aaaagccagc accctgatgc
    11761 gacctcgttt tagtctacgt ttatctgtct ttacttaatg tcctttgtta caggccagaa
    11821 agcataactg gcctgaatat tctctctggg cccactgttc cacttgtatc gtcggtctga
    11881 taatcagact gggaccacgg tcccactcgt atcgtcggtc tgattattag tctgggacca
    11941 cggtcccact cgtatcgtcg gtctgattat tagtctggga ccacggtccc actcgtatcg
    12001 tcggtctgat aatcagactg ggaccacggt cccactcgta tcgtcggtct gattattagt
    12061 ctgggaccat ggtcccactc gtatcgtcgg tctgattatt agtctgggac cacggtccca
    12121 ctcgtatcgt cggtctgatt attagtctgg aaccacggtc ccactcgtat cgtcggtctg
    12181 attattagtc tgggaccacg gtcccactcg tatcgtcggt ctgattatta gtctgggacc
    12241 acgatcccac tcgtgttgtc ggtctgatta tcggtctggg accacggtcc cacttgtatt
    12301 gtcgatcaga ctatcagcgt gagactacga ttccatcaat gcctgtcaag ggcaagtatt
    12361 gacatgtcgt cgtaacctgt agaacggagt aacctcggtg tgcggttgta tgcctgctgt
    12421 ggattgctgc tgtgtcctgc ttatccacaa cattttgcgc acggttatgt ggacaaaata
    12481 cctggttacc caggccgtgc cggcacgtta accgggctgc atccgatgca agtgtgtcgc
    12541 tgtcgacgag ctcgcgagct cggacatgag gttgccccgt attcagtgtc gctgatttgt
    12601 attgtctgaa gttgttttta cgttaagttg atgcagatca attaatacga tacctgcgtc
    12661 ataattgatt atttgacgtg gtttgatggc ctccacgcac gttgtgatat gtagatgata
    12721 atcattatca ctttacgggt cctttccggt gatccgacag gttacggggc ggcgacctcg
    12781 cgggttttcg ctatttatga aaattttccg gtttaaggcg tttccgttct tcttcgtcat
    12841 aacttaatgt ttttatttaa aataccctct gaaaagaaag gaaacgacag gtgctgaaag
    12901 cgagcttttt ggcctctgtc gtttcctttc tctgtttttg tccgtggaat gaacaatgga
    12961 agtccgagct catcgctaat aacttcgtat agcatacatt atacgaagtt atattcgatg
    13021 cggccgcaag gggttcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    13081 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cacacagatg
    13141 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc agctgcgcaa ctgttgggaa
    13201 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
    13261 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
    13321 agtgaattgt aatacgactc actatagggc gaattcgagc tcggtacccg gggatcctct
    13381 agagtcgacc tgcaggcatg caagcttgag tattctatag tctcacctaa atagcttggc
    13441 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    13501 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    13561 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    13621 ttaatgaatc ggccaacgcg aaccccttgc ggccgcccgg gccgtcgacc aattctcatg
    13681 tttgacagct tatcatcgaa tttctgccat tcatccgctt attatcactt attcaggcgt
    13741 agcaaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc
    13801 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa
    13861 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
    13921 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
    13981 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag
    14041 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
    14101 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
    14161 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
    14221 tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
    14281 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
    14341 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
    14401 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
    14461 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
    14521 agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct
    14581 tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag
    14641 gtatttattc gcgataagct catggagcgg cgtaaccgtc gcacaggaag gacagagaaa
    14701 gcgcggatct gggaagtgac ggacagaacg gtcaggacct ggattgggga ggcggttgcc
    14761 gccgctgctg ctgacggtgt gacgttctct gttccggtca caccacatac gttccgccat
    14821 tcctatgcga tgcacatgct gtatgccggt ataccgctga aagttctgca aagcctgatg
    14881 ggacataagt ccatcagttc aacggaagtc tacacgaagg tttttgcgct ggatgtggct
    14941 gcccggcacc gggtgcagtt tgcgatgccg gagtctgatg cggttgcgat gctgaaacaa
    15001 ttatcctgag aataaatgcc ttggccttta tatggaaatg tggaactgag tggatatgct
    15061 gtttttgtct gttaaacaga gaagctggct gttatccact gagaagcgaa cgaaacagtc
    15121 gggaaaatct cccattatcg tagagatccg cattattaat ctcaggagcc tgtgtagcgt
    15181 ttataggaag tagtgttctg tcatgatgcc tgcaagcggt aacgaaaacg atttgaatat
    15241 gccttcagga acaatagaaa tcttcgtgcg gtgttacgtt gaagtggagc ggattatgtc
    15301 agcaatggac agaacaacct aatgaacaca gaaccatgat gtggtctgtc cttttacagc
    15361 cagtagtgct cgccgcagtc gagcgacagg gcgaagcccg tttaaacggg gtgggcgaag
    15421 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt
    15481 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg
    15541 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
    15601 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
    15661 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
    15721 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
    15781 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
    15841 gcatccgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
    15901 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
    15961 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
    16021 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
    16081 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
    16141 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttggagtt
    16201 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
    16261 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
    16321 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
    16381 acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag atccttggcg
    16441 gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag
    16501 ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat cgccatgtaa
    16561 gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc cagatagccc
    16621 agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc tacgtgttcc
    16681 gcttccttta gcagcccttg cgccctgagt gcttgcggca gcgtgaagct ttaatcatga
    16741 ttacgccaag ctcgcgaggc ctcgagatct atcgatgcat gccatggtac ccgggagctc
    16801 gaattctaga agcttctgca gacgcgtcga cgtcatatgg atccgattcg acctgcaggc
    16861 atgcaagctg tacgcctcag ctaccattct tgcagccgcg ttcagggtct tgacgtgttc
    16921 tttgtatcta tcagcaaact gagaacccaa ttctggatca tccttgtaac gccaactctc
    16981 cgaccattcc gaaggaacgc cttgtagtcc tcctgagccg ttagggtatg tctttagtcc
    17041 aatcaaatgg gacatttgca gacaggaacg cacaagtgtc tcacgtcgct tctctatttc
    17101 aatatctaaa aattcttcac tcttatcgtt gcttgacata ttaaaactgt ctaactgatc
    17161 cagttcactt atatggaaag tatcactaaa gtcatggctt ctcagaaatt caatacttcc
    17221 cattgatggg gaccggttaa ccaaatcgat aaaaattttt ctgtcatgtt tcagataagt
    17281 ttttggcgcg ttgattttat aataaagccg ccattcttgt gccaacaacg ccacgaaaag
    17341 agccacagca gcagcggcca catcccattg aaaatctttc gccagaccaa ccgccaggag
    17401 aaccaagccg aaatagttag cccagcgaac taaaaaacta tctataaacg tattaagaaa
    17461 cgttcgggtc atatttccgt gaatcatatc aggacgtaaa tttgcacatg acgcatttcc
    17521 gggggctttt atccctcgga cttcttttca ggccccttct cggcctttct gtgaccctgt
    17581 tttttcaggc cgcgaactgc gcctggtcgg gcctgacccc cacacgctgg ccttcgtact
    17641 cacgcagcct gcccagcagg tcggccagcg cggcattgac cacggcggcg aggttgcggg
    17701 gcggcgttcc gcctgcggtc tggtcgtgct tcacgtcgcg cagcacgcgc accagcaccg
    17761 cgcccacgtc gtcggacacg tcccggccag cgtcggcggc gcgggtgatg ttccaaatga
    17821 gtttccgcca gaagcccagc gagtcggccc cgtccccgaa agctgccgct agcgcccgcg
    17881 cctgcctgtc cacgatctcg gcgcggtagg ccgggcgggt ttcccggtgg gcgtcggcaa
    17941 gctcccacac gacgttttca gcgagggagg gcgcgggccg gacagtcatg ttatcggagg
    18001 gggagggaag agcggatttt atcgcccagg tgctcacaac ttcgtatgtc acggcaaccg
    18061 gggcgctttt ttcgcgcagt tccccgcctg tcacatcctc acagggttct tgactctccg
    18121 gcacgctctt agggagaggg tggagcatgt tgtagacggt gcgcccggcc ttcgcgtcgg
    18181 cgttcaggtc gcgccagttc cggccccaat cgtcatgcat caggcggaca tatcccgcct
    18241 tcccttccag cacgcgcctc ggcttgagcg tcacggccca cagcgtcccg gttgccacag
    18301 actcgccgcg caggtcgccc atgtgggcat cacaggccac cagcccgacc cggcgcagat
    18361 attgaaggtt ctcgtagaag gcggacttct tcagacccac atgcaccatg agcagctcgg
    18421 cgggcaggtg gaacacagcg cgggtgacgt gttccgcata gccgcaggcg cgggccacgt
    18481 ccagcgccac cgagaacagg gcgcggaaga tgcgccgggc cgactcgcgg cagggggcgt
    18541 catctaggca ggcggtgagg gtctgcacca gttcggcgcg tgtggcgatg gtcaacggcg
    18601 cgggcgcggg ctgggactgc tgggcgatgg tgggcacggg cggcggcgag atggaccggg
    18661 ccgagctgga aatttcccgt gtgagcgcag gtttaggcgc atggttacct tcctgcagtg
    18721 aaatctgcga aatcaggccc gcttcgagga gtcggaccat gaatggattg tgtctcaaga
    18781 aaaaagcctc ccctcttgtc agaggggagg gtcccggtct accatgctct cagcgacgag
    18841 atagctgggg tttgtgaccc tgcactctga caaagccctc accgaaaggt gggggtttag
    18901 tcatttggcc tagtaaagca gaacagaggc gatttgtcac gcaaaggccc gccgcccgag
    18961 gggaaacagg cggcgggcca atggctcggc gttgttagct gggggtcgca ccagggaagc
    19021 acagaagccc ttccctgagc agggtttcac cgtgtggtgt cccttcgtcc ttgaaggcga
    19081 actggtaggc caggccgtaa tgctcctgca ccatcttttg ctcatccacc gcgacaaaga
    19141 aacggcgggt gttcgccggg ctgctgaggg tctgccactc gccggacgcg cccaccgcat
    19201 aggcgtctcc ggctccacat tcctcggcct gctgatactg atacttcgtc cacagcatca
    19261 ccgccttatc gggcacctgc gcgaagctca ccagcgcccc gaaccgcccg gcgctcgtgg
    19321 tgaaggtgga cgactgcacc atgtcggaca gttccagcgt ggcaggcgcg ggcgtgttcg
    19381 tcggcgcgta caccgtcccc gcctcgcaca cgaccagcgg ggcggccccc gtgccgaact
    19441 cggcggcgct gccgtttgcc agcgtgagcg ccgaagacgc caccgcgcag gtacccatgg
    19501 gtgccgacca ccagcaggtc cacgccgtgc gccgcctcgg tgagcacctc gattggacgg
    19561 cccctcaggg tctgaaccct cccctgcacg cccgcctccc cgatgagtgt ctgcgccgtc
    19621 tgctgcgcct gccgggcatt ctgctccagc atctcccgga cgtagtcggg attcagtccc
    19681 gcccactgaa cgaagccgct gaccggcggc tcctcgatca cagtcacgac ctccagctcg
    19741 gcgcctgccg ccttcccgag cgttatggcc tgcttcaagg cgcgctggct gggctcactt
    19801 ccgtcaaagg ccaccacgaa tcgcatccgt cctcctccct gaccagtcag gttcagcaag
    19861 cagggtaagc ctctcccagc actccggggc ttcacacggc cttgggtctt ttctgcatac
    19921 ccatgagcct cagtacccga cagtttccgg cagggcttga aaaagttcgg ctgacacgcg
    19981 agaactggac gcgtctcccg atgtcctggc caagttgacc cgctgcttca ccgcgcactt
    20041 cccggagttc cgcaagaact aggtcgagct gctctccctc atggtcctcg ccctccttag
    20101 gggcaaggac gtccggcatg ctaaactcgc cgcgcgcttc cccggaagcg cgcacaccgc
    20161 ctccgtcatc cggcgggtgg aacgcttctt cgaccgtcat cctcttcggc cagctgatgt
    20221 cgcccgggtc gttctgacgc tccttcccgc cgcgcagcca cgcgaattta tccttgaccg
    20281 gaccaactgg aagtatgggc agacggacgt gaacgtcttg ttgctggccg tcatttggcg
    20341 ggacgtcgcc atccccctgc tctacgagtt gctgccccat gggggcagca gcgacaccga
    20401 gattcggcac accctgatgg acgatgccct gtgcctgctg tccgccgctg acatccgggt
    20461 gctgtatgcc gaccgcgaat tcccagcttg agtaggacaa atccgccgag cttcgacgag
    20521 attttcagga gctaaggaag ctaaagcgag ggcctgaggg ccatggagac cgagggccct
    20581 ggacattgag aatgattctc aatatggtgc agggagcttc gggcctcttg ccgcgcagca
    20641 gagccagcga ggcgaaggag agtgtatgga aaaaaagatt actggttaca ccaccgtgga
    20701 cattagtcag tggcatcgta aggagcattt cgaagcgttt caaagcgtgg cacagtgcac
    20761 gtacaaccag accgttcaac tcgacatcac cgccttcctg aagaccgtga agaaaaacaa
    20821 gcacaagttt taccccgcct ttattcacat ccttgcccgc ttgatgaatg ctcaccctga
    20881 gctccgcatg gccatgaaag acggggagct ggtcatctgg gatagcgtgc acccgtgtta
    20941 caccgtcttc cacgagcaga ccgagacgtt ctcgtctctc tggtcggagt atcacgacga
    21001 cttccggcag ttcctgcaca tctacagcca ggacgtcgcg tgctacggcg agaacctcgc
    21061 gtacttcccg aagggcttta tcgaaaatat gttttttgtg tccgccaacc cctgggtgag
    21121 cttcaccagc ttcgacctga acgtcgccaa catggacaac tttttcgccc cggtcttcac
    21181 gatgggaaag tattacacgc agggcgacaa agtgctgatg cccctggcga tccaggtgca
    21241 ccacgcggtg tgcgacgggt tccacgtggg ccgcatgctg aacgaactgc agcagtactg
    21301 cgacgaatgg cagggcggcg cgtgataggc agaaaaaatc cccccggtgg caatccgggg
    21361 ggttttttta aatcttccgc tgcataaccc tgcttcgggg tcattatagc gattttttcg
    21421 gtatatccat cctttttcgc acgatataca ggattttgcc aaagggttcg tgtagacttt
    21481 ccttggtgta tccaacggcg tcagccgggc aggataggtg aagtaggccc acccgcgagc
    21541 gggtgttcct tcttcactgt cccttattcg cacctggcgg tgctcaacgg gaatcctgct
    21601 ctgcgaggct ggccggctac cgccggcgta acagatgagg gcaagcggat ggctgatgaa
    21661 accaagccaa ccaggaaggg cagcccacct atcaaggtgt actgccttcc agacgaacga
    21721 agagcgattg aggaaaaggc ggcggcggcc ggcatgagcc tgtcggccta cctgctggcc
    21781 gtcggccagg gctacaaaat cacgggcgtc gtggactatg agcacgtccg cgagctggcc
    21841 cgcatcaatg gcgacctggg ccgcctgggc ggcctgctga aactctggct caccgacgac
    21901 ccgcgcacgg cgcggttcgg tgatgccacg atcctcgccc tgctggcgaa gatcgaagag
    21961 aagcaggacg agcttggcaa ggtcatgatg ggcgtggtcc gcccgagggc agagccatga
    22021 cttttttagc cgctaaaacg gccggggggt gcgcgtgatt gccaagcacg tccccatgcg
    22081 ctccatcaag aagaggcact tcgagctgta agtacatcac cgacgagcaa ggcaagacga
    22141 tc
//
