LOCUS       sequence-375156-       10676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct 
            repeat and a nd a unique sgRNA scaffold variant with a capture 
            sequence. Cloning guide RNAs using BsmBI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10676)
  AUTHORS   Wessels HH, Mendez-Mancilla A, Hao Y, Papalexi E, Mauck WM 3rd, Lu 
            L, Morris JA, Mimitou EP, Smibert P, Sanjana NE, Satija R
  TITLE     Efficient combinatorial targeting of RNA transcripts in single cells
            with Cas13 RNA Perturb-seq.
  JOURNAL   Nat Methods. 2023 Jan;20(1):86-94. doi: 10.1038/s41592-022-01705-x. 
            Epub 2022 Dec 22.
  PUBMED    36550277
REFERENCE   2  (bases 1 to 10676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-022-01705-x"; journalName: "Nat Methods"; date: 
            "2023-01"; volume: "20"; issue: "1"; pages: "86-94"
COMMENT     Sequence Label: lentiGuideFB-Puro-A
FEATURES             Location/Qualifiers
     source          1..10676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(225..329)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(404..423)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        595..974
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        976..1174
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1129..1149
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1192..1372
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1419..1544
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2037..2270
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2455..2499
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2797..2914
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2965..3213
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2965..2985
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3136..3155
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        5283..5494
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             5510..6109
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5510..5529)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6006..6026
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6125..6713
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6178..6198)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6596..6607)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6785..7018
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7044..7061)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7050..7274
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7320..7748
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7407..7426)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7617..7638
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7757..7777)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7762..8091
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7942..8077
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8004..8023
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8139..8186
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8205..8579
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8709..8830
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8746..8765)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8800..8819
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8879..8895)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8879..8895)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8892..8914)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8903..8919
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8927..8933,8934..8951,8952..8957))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8972..8993
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9110..9127)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9281..9869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9361..9380)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10040..10676,1..155,156..224))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     join(10663..10676,1..6)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
       61 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
      121 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
      181 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
      241 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
      301 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
      361 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
      421 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      481 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      541 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      601 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      661 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      721 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      781 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      841 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      901 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      961 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     1021 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     1081 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     1141 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
     1201 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     1261 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     1321 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1381 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1441 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1501 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1561 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1621 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1681 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1741 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1801 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1861 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1921 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1981 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     2041 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     2101 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     2161 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     2221 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     2281 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     2341 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2401 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2461 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2521 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2581 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2641 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2701 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg
     2761 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     2821 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     2881 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     2941 tccagtttgg ttaattaagg taccgagggc ctatttccca tgattccttc atatttgcat
     3001 atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata
     3061 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
     3121 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
     3181 gctttatata tcttgtggaa aggacgaaac accggagacg gttgtaaatg agcacacaaa
     3241 atacacatgc taaaatatta tattctatga cctttataaa atcaaccaaa atcttctttt
     3301 taataacttt agtatcaata attagaattt ttatgttcct ttttgcaaac ttttaataaa
     3361 aatgagcaaa ataaaaaaac gctagtttta gtaactcgcg ttgttttctt cacctttaat
     3421 aatagctact ccaccacttg ttcctaagcg gtcagctcct gcttcaatca ttttttgagc
     3481 atcttcaaat gttctaactc caccagctgc tttaactaaa gcattgtctt taacaactga
     3541 cttcattagt ttaacatctt caaatgttgc acctgatttt gaaaatcctg ttgatgtttt
     3601 aacaaattct aatccagctt caacagctat ttcacaagct ttcatgattt cttcttttgt
     3661 taataaacaa ttttccataa tacatttaac aacatgtgat ccagctgctt tttttacagc
     3721 tttcatgtct tctaaaacta attcataatt tttgtctttt aatgcaccaa tatttaatac
     3781 catatcaatt tctgttgcac catctttaat tgcttcagaa acttcgaatg cttttgtagc
     3841 tgttgtgcat gcacctagag gaaaacctac aacatttgtt attcctacat ttgtgccttt
     3901 taataattct ttacaatagc ttgttcaata tgaattaaca caaactgttg caaaatcaaa
     3961 ttcaattgct tcatcacata attgtttaat ttcagctttc gtagcatctt gttttaataa
     4021 tgtgtgatct atatatttgt ttagtttcat tttttctcct atatattcat ttttaatttt
     4081 aattctttaa taatttcgtc tactttaact ttagcgtttt gaacagattc accaacacct
     4141 ataaaataaa tttttagttt aggttcagtt ccacttgggc gaacagcaaa tcatgactta
     4201 tcttctaaat aaaattttag taagtcttgt cctggcatat tatacattcc atcgatgtag
     4261 tcttcaacat taacaacttt aagtccagca atttgagtta agggtgttgc tctcaatgat
     4321 ttcattaatg gttcaatttt taatttcttt tcttctggtt taaaattcaa gtttaaagtg
     4381 aaagtgtaat atgcacccat ttctttaaat aaatcttcta aatagtctac taatgtttta
     4441 ttttgttttt tataaaatca agcagcctct gctattaata tagaagcttg tattccatct
     4501 ttatctctag ctgagtcatc aattacatat ccataacttt cttcataagc aaaaacaaaa
     4561 tttaatccgt tatcttcttc tttagcaatt tctctaccca ttcatttaaa tccagttaaa
     4621 gtttttacaa tattaactcc atatttttca tgagcgattc tatcacccaa atcacttgtt
     4681 acaaaacttg aatatagagc cggatttttt ggaatgctat ttaagcgttt tagatttgat
     4741 aattttcaat caattaaaat tggtcctgtt tgatttccat ctaatcttac aaaatgacca
     4801 tcatgtttta ttgccattcc aaatctgtca gcatctgggt cattcataat aataatatct
     4861 gcatcatgtt taataccata ttcaagcggt atttttcatg caggatcaaa ttctggattt
     4921 ggatttacaa catttttaaa tgtttcatct tcaaatgcat gctctttaac ctcaataacg
     4981 ttatatcctg attcacgtaa tatttttggg gtaaatttag ttcctgttcc attaactgcg
     5041 ctaaaaataa tttttaaatc ttttttagct tcttgctctt ttttgtacgt ctctgtttaa
     5101 gagctaagct ggaaacagca tagcaagttt aaataaggct agtccgttat caacttggcc
     5161 gctttaaggc cggtcctagc aaggccaagt ggcaccgagt cggtgctttt ttaagcttgg
     5221 cgggatccgg tacgctagct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     5281 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     5341 atccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     5401 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     5461 tctttttcgc aacgggtttg ccgccagaac acaggaccgg ttctagagca tgaccgagta
     5521 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     5581 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     5641 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     5701 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     5761 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     5821 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     5881 cctggccacc gtcggagtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     5941 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     6001 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     6061 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttaagt
     6121 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6181 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6241 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6301 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6361 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     6421 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     6481 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     6541 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     6601 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     6661 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
     6721 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     6781 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     6841 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6901 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     6961 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
     7021 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     7081 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     7141 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     7201 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     7261 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     7321 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     7381 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     7441 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     7501 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     7561 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     7621 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     7681 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     7741 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     7801 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     7861 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     7921 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     7981 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     8041 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     8101 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
     8161 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
     8221 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
     8281 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
     8341 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
     8401 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
     8461 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
     8521 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
     8581 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     8641 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     8701 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8761 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8821 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     8881 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8941 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     9001 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     9061 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     9121 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     9181 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     9241 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     9301 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     9361 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     9421 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     9481 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     9541 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     9601 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9661 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9721 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9781 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9841 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9901 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9961 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    10021 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    10081 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    10141 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    10201 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    10261 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    10321 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    10381 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    10441 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    10501 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    10561 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    10621 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgcca
//
