LOCUS       sequence-375157-        9104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct 
            repeat and a unique sgRNA scaffold variant with a capture sequence. 
            Cloning guide RNAs using BsmBI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9104)
  AUTHORS   Wessels HH, Mendez-Mancilla A, Hao Y, Papalexi E, Mauck WM 3rd, Lu 
            L, Morris JA, Mimitou EP, Smibert P, Sanjana NE, Satija R
  TITLE     Efficient combinatorial targeting of RNA transcripts in single cells
            with Cas13 RNA Perturb-seq.
  JOURNAL   Nat Methods. 2023 Jan;20(1):86-94. doi: 10.1038/s41592-022-01705-x. 
            Epub 2022 Dec 22.
  PUBMED    36550277
REFERENCE   2  (bases 1 to 9104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-022-01705-x"; journalName: "Nat Methods"; date: 
            "2023-01"; volume: "20"; issue: "1"; pages: "86-94"
COMMENT     Sequence Label: lentiGuideFB-Puro-B
FEATURES             Location/Qualifiers
     source          1..9104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(113..217)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(292..311)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        483..862
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        864..1062
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1017..1037
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1080..1260
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1307..1432
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1925..2158
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2343..2387
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2685..2802
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3599..3810
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             3826..4425
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3826..3845)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4322..4342
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4441..5029
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4494..4514)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4912..4923)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5101..5334
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5360..5377)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5366..5590
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5636..6064
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5723..5742)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5933..5954
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6073..6093)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6078..6407
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6258..6393
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6320..6339
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6455..6502
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6521..6895
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7025..7146
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7062..7081)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7116..7135
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7195..7211)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7195..7211)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7208..7230)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7219..7235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7243..7249,7250..7267,7268..7273))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7288..7309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7426..7443)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7597..8185)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7677..7696)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8356..9104,1..43,44..112))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8979..8998
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
       61 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
      121 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
      181 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
      241 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
      301 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
      361 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
      421 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
      481 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
      541 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      601 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      661 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      721 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      781 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      841 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      901 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      961 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     1021 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg
     1081 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     1141 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     1201 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     1261 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     1321 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     1381 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     1441 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     1501 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     1561 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     1621 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     1681 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     1741 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     1801 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1861 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1921 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1981 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     2041 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     2101 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     2161 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     2221 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     2281 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     2341 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     2401 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     2461 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     2521 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     2581 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     2641 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2701 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2761 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2821 agcagagatc cagtttggtt aattaactcg aggtacccgt gaccgagctt gtctgcccgc
     2881 gcagtccact agacagacgc gcagaggtcg ggcgggccta gggccaattt cccatggttc
     2941 ccttcatttg catatatgat gtaataaggt tatggagact attaaactta gccctaatca
     3001 aactatatga tgataatgtg tgtggtacaa aaggtcataa cttattatat actttgaaac
     3061 ttaaaaaagg gttacagttt agtcaccata actgtaaaat tttttctatt cttagcttta
     3121 tatagttctt gagaggccat gtttatggca ccggagacgg ttgtaaatga gcacacaaaa
     3181 tacacatgct aaaatattat attctatgac ctttataaaa tcaaccaaaa tcttcttttt
     3241 aataacttta gtatcaataa ttagaatttt tatgttcctt tttgcaaact tttaataaaa
     3301 atgagcaaaa taaaaaaacg ctagtaaatt tagttcctgt tccattaact gcgctaaaaa
     3361 taatttttaa atctttttta gcttcttgct cttttttgta cgtctctgtt tgagagctaa
     3421 gcagaaagct gcatagcaag ttcaaataag gctagtccgt acacaacttg gccgctttaa
     3481 ggccggtcct agcaaggcca agtggcagcc gagtcggctg ctttttttaa gcttggcggg
     3541 atccggtacg ctagctaggt cttgaaagga gtgggaattg actccggtgc ccgtcagtgg
     3601 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgatcc
     3661 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3721 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3781 tttcgcaacg ggtttgccgc cagaacacag gaccggttct agagcatgac cgagtacaag
     3841 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     3901 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     3961 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     4021 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     4081 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     4141 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     4201 gccaccgtcg gagtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     4261 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     4321 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     4381 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaacgcg ttaagtcgac
     4441 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4501 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4561 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4621 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4681 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4741 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4801 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     4861 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4921 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4981 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     5041 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     5101 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     5161 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5221 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5281 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     5341 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     5401 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     5461 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     5521 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     5581 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     5641 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5701 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5761 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5821 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5881 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5941 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6001 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6061 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     6121 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     6181 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     6241 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6301 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     6361 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     6421 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     6481 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     6541 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     6601 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     6661 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     6721 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     6781 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     6841 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     6901 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     6961 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     7021 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7081 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7141 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     7201 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7261 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7321 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7381 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7441 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7501 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7561 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7621 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7681 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7741 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7801 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7861 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7921 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7981 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8041 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8101 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8161 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8221 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8281 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8341 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8401 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8461 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8521 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8581 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8641 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8701 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8761 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8821 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8881 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8941 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9001 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9061 accgctgttg agatccagtt cgatgtaacc cactcgtgca ccca
//
