LOCUS       sequence-375164-        8249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ddFP-B1-inserted mouse N-cadherin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Kanadome T, Hayashi K, Seto Y, Eiraku M, Nakajima K, Nagai T, 
            Matsuda T
  TITLE     Development of intensiometric indicators for visualizing N-cadherin 
            interaction across cells.
  JOURNAL   Commun Biol. 2022 Oct 7;5(1):1065. doi: 10.1038/s42003-022-04023-2.
  PUBMED    36207396
REFERENCE   2  (bases 1 to 8249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-10-7"; volume: "5(1):1065. doi"; pages: " 
            10.1038/s42003-022-04023-2"
COMMENT     Sequence Label: pCAG-NCad-B1
FEATURES             Location/Qualifiers
     source          1..8249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        130..509
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          760..1776
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1700..1722
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1784..1803
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(5396..5415)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5461..5516
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5515..5534)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5877..5893)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5877..5893)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5890..5912)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5901..5917
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5925..5931,5932..5949,5950..5955))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5970..5991
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6050..6245
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6096..6231
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6251..6385
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6288..6307)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6342..6361
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6453..6470)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6624..7212)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6704..6723)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7383..8174,8175..8243))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8006..8025
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8244..8249,1..99))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
       61 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
      121 acctgggtcg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
      181 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      241 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      301 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      361 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      421 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      481 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc
      541 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt
      601 gtgcagcgat gggggcgggg gggggggggg gggggggggg gcggggcgag gcggagaggt
      661 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
      721 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
      781 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
      841 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
      901 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
      961 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     1021 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
     1081 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     1141 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
     1201 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
     1261 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg
     1321 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     1381 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
     1441 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     1501 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
     1561 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     1621 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     1681 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
     1741 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     1801 gtgctgtctc atcattttgg caaagaattc accatgtgcc ggatagcggg agcgccgcgg
     1861 accctgctgc cgcttctggc ggccttgctt caggcgtctg tggaggcttc tggtgaaatt
     1921 gcattatgca agactggatt tcctgaagat gtttacagcg cagtcttacc gaaggatgtg
     1981 cacgaaggac agccccttct caatgtgaaa ttcagcaact gcaatagaaa aaggaaagtt
     2041 cagtatgaaa gcagcgagcc agcagatttc aaggtggacg aggacggcac ggtgtatgct
     2101 gtgagaagct tccctctcac tgcagagcag gcaaagttcc tgatatatgc ccaagacaaa
     2161 gaaacccagg aaaagtggca ggtagctgta aacctgagcc gggagccaac cctgactgag
     2221 gagcctatga aggaaccaca tgaaattgaa gaaatagtat tccctagaca acttgccaag
     2281 cacagtggag ctctacaaag gcagaagaga gactgggtca tcccgccaat caacttgcca
     2341 gaaaactcca gaggaccctt tcctcaagag cttgtcagaa tcaggtctga tagagataaa
     2401 aacctttccc tgagatacag cgtcactggg ccaggagctg accagcctcc aacgggcatc
     2461 ttcattatca accccatctc aggacagctg tcagtcacaa agcctctgga tcgagagctg
     2521 atagcccggt ttcacttgag agcacatgca gtggacatca atggcaatca agtggagaac
     2581 cccattgaca ttgtcatcaa tgttattgac atgaatgata acagacctga gtttctgcac
     2641 caggtttgga atgggtctgt tccagaggga tcaaagcctg ggacgtatgt gatgacggtc
     2701 actgccattg atgcggatga tccaaatgcc ctgaatggaa tgctgcggta caggatcctg
     2761 ggctcagcta caatcaaaga gtttatgcgg ttcaaggtcc gcatggaagg gagtatgaat
     2821 ggacatgagt tcgagattga aggcgaaggg gagggaaggc catacgaagg tacacaaact
     2881 gccaagctca aagtaaccaa aggtggacca ttgccctttg catgggacat actctctcca
     2941 cagatcatgt atggcagcaa ggcatatgta cgccaccctg ccgatatccc cgattacaag
     3001 aaattgccct ttcctgaggg gttcaaatgg gaaagggtaa tgaattttga ggatgggggg
     3061 cttgtaaccg ttacccagga ctcttctctg caagacggca ccctgatatg caaggttaaa
     3121 attcgcggca ctaatttccc tcctgatggg ccagtgatgc aaaagaaaac aatgggatgg
     3181 gaagcctcaa cagagatgct gtatcccaaa gacggggtat tgaagggcca tagctatcaa
     3241 gcactgaaac ttaaggatgg tgggcactac ctggtcgagt ttgagacaat atatatggca
     3301 aagaaaccag tacagctccc tggggactat tgtgtcgaca caaaactcga catcacatcc
     3361 cacaatgaag actatactat tgtggagcaa tatggaagga gtgagggacg gcatcggctt
     3421 gggatggacg agcgctataa gggctcagct agccagatcc tgtcccaggc gcccagcaca
     3481 ccttcaccca acatgtttac aatcaacaat gagactgggg acatcatcac tgtggcagct
     3541 ggtctggatc gagagaaagt gcaacagtat acgttaataa ttcaagccac agacatggaa
     3601 ggcaatccca cttatggcct ttcaaacaca gccacagccg tcatcacggt gacagatgtc
     3661 aatgacaatc ctccagagtt tactgccatg actttctacg gagaagtccc tgagaacagg
     3721 gtggacgtca ttgtagccaa cctaactgtc acggacaaag atcagcccca cacgccggcc
     3781 tggaatgcgg catacagaat cagtggtgga gaccctacag gaaggtttgc catcctgaca
     3841 gaccccaaca gcaatgatgg gctagtcaca gtggtaaaac caattgactt tgaaacgaat
     3901 aggatgtttg tccttactgt tgctgcagaa aaccaagtgc cattagctaa aggcattcag
     3961 cacccacctc agtcgacagc cactgtgtct gtgacagtta ttgatgtcaa tgaaaatcct
     4021 tattttgccc caaatcctaa aatcattcgc caagaggaag gcctccacgc aggtaccatg
     4081 ctgaccacgc tcactgctca ggaccccgat cgatatatgc aacagaatat cagatacaca
     4141 aaattgtctg atcctgccaa ctggctgaaa atagaccccg tgaatgggca gatcactact
     4201 attgccgttt tggacagaga atcgccaaat gtaaaaaaca acatctataa tgctaccttc
     4261 cttgcttctg acaatggaat cccgcctatg agtgggacag gaacactgca aatctattta
     4321 cttgatatca atgacaacgc ccctcaggtg ttacctcaag aggcggagac ctgtgaaact
     4381 ccagaaccca actcaattaa catcacagca cttgattatg acatagaccc aaacgccggg
     4441 ccgttcgcgt ttgatcttcc cttatctcca gtgactatta aaagaaactg gaccatcaac
     4501 cggcttaatg gtgattttgc tcagctcaat ttaaagataa aatttttgga agctggtatc
     4561 tatgaagttc ccatcattat cacagattca gggaatcccc ccaagtccaa catttccatc
     4621 ctgcgtgtga aagtttgtca gtgtgactcc aatggagact gcacggacgt ggacaggatc
     4681 gtgggtgcag ggcttggcac gggcgccatc atcgctatcc ttctgtgtat catcatcctg
     4741 ctgatccttg ttctcatgtt tgtggtatgg atgaaacggc gggataaaga gcgccaagcc
     4801 aagcagcttt taattgaccc agaagatgat gtaagagata atatattgaa atatgatgaa
     4861 gaaggtggag gagaagaaga ccaggactat gacttgagcc agctccagca accagatact
     4921 gtggagcctg atgccatcaa gcccgtggga atcagacggc tagacgagag gcctatccat
     4981 gctgagccac agtacccagt ccgatccgca gccccacacc ctggggatat tggggacttc
     5041 attaatgagg gccttaaagc tgctgacaac gaccccacgg cgccaccgta tgactccctc
     5101 ttagtctttg actacgaggg cagcggctcc acggctggct ccttgagctc cctcaactcc
     5161 tccagtagcg gtggggacca ggactatgac tacctgaatg actggggacc ccgcttcaag
     5221 aaactggcgg acatgtacgg cggtggtgac gacaagcttt acccatacga cgtcccagac
     5281 taccggtaac tcgaggcggc cgcttttttc cttaattcac tcctcaggtg caggctgcct
     5341 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt
     5401 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct
     5461 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
     5521 gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt
     5581 tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc
     5641 agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag
     5701 gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt
     5761 ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg
     5821 tccctcttct cttatggaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca
     5881 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     5941 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6001 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt
     6061 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6121 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6181 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     6241 gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     6301 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     6361 caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga
     6421 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6481 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6541 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6601 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6661 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6721 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6781 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6841 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6901 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6961 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7021 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     7081 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7141 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7201 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7261 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7321 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7381 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7441 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7501 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7561 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7621 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7681 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7741 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7801 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7861 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7921 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7981 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8041 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     8101 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8161 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8221 acacggaaat gttgaatact catactctt
//
