LOCUS       sequence-375165-        9667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to constitutively express YAP under control of the
            human PGK promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Pearson JD, Huang K, Pacal M, McCurdy SR, Lu S, Aubry A, Yu T, 
            Wadosky KM, Zhang L, Wang T, Gregorieff A, Ahmad M, Dimaras H, 
            Langille E, Cole SPC, Monnier PP, Lok BH, Tsao MS, Akeno N, Schramek
            D, Wikenheiser-Brokamp KA, Knudsen ES, Witkiewicz AK, Wrana JL, 
            Goodrich DW, Bremner R
  TITLE     Binary pan-cancer classes with distinct vulnerabilities defined by 
            pro- or anti-cancer YAP/TEAD activity.
  JOURNAL   Cancer Cell. 2021 Jul 13. pii: S1535-6108(21)00338-X. doi: 
            10.1016/j.ccell.2021.06.016.
  PUBMED    34270926
REFERENCE   2  (bases 1 to 9667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2021 Jul 13. pii: S1535-6108(21)00338-X. doi: 
            10.1016/j.ccell.2021.06.016."
COMMENT     Sequence Label: PGKp-GFP-YAP
FEATURES             Location/Qualifiers
     source          1..9667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     430..448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             531..596
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(2112..2136)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(2179..2753,2754..2756,2757..2765)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2346..2363)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2573..2592
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2766..2768,2769..2771,2772..3482)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2766..2768,2769..2771,2772..3482)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2811..2832)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3072..3091)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3419..3440
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3492..3545
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3546..4145
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3546..3565)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4042..4062
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4273..4506
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4578..4699
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4615..4634)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4669..4688
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4739..4874
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4894..4913)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4895..4913)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4923..4940)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4923..4939)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4932..4954)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5081..5536
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5168..5187)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5378..5399
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5562..5666
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5667..5735,5736..6527)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5885..5904)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6698..7286
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7187..7206
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7440..7457
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7574..7595
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7610..7615,7616..7633,7634..7640)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7648..7664
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7653..7675
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7672..7688
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7672..7688
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7707..7727
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7709..7727
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7755..7981
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7982..8162
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8209..8334
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8827..9060
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9245..9289
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9502..9619
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggagcatg gactacaaag
      541 accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagatgg
      601 atcccgggca gcagccgccg cctcaaccgg ccccccaggg ccaagggcag ccgccttcgc
      661 agcccccgca ggggcagggc ccgccgtccg gacccgggca accggcaccc gcggcgaccc
      721 aggcggcgcc gcaggcaccc cccgccgggc atcagatcgt gcacgtccgc ggggactcgg
      781 agaccgacct ggaggcgctc ttcaacgccg tcatgaaccc caagacggcc aacgtgcccc
      841 agaccgtgcc catgaggctc cggaagctgc ccgactcctt cttcaagccg ccggagccca
      901 aatcccactc ccgacaggcc agtactgatg caggcactgc aggagccctg actccacagc
      961 atgttcgagc tcattcctct ccagcttctc tgcagttggg agctgtttct cctgggacac
     1021 tgacccccac tggagtagtc tctggcccag cagctacacc cacagctcag catcttcgac
     1081 agtcttcttt tgagatacct gatgatgtac ctctgccagc aggttgggag atggcaaaga
     1141 catcttctgg tcagagatac ttcttaaatc acatcgatca gacaacaaca tggcaggacc
     1201 ccaggaaggc catgctgtcc cagatgaacg tcacagcccc caccagtcca ccagtgcagc
     1261 agaatatgat gaactcggct tcaggtcctc ttcctgatgg atgggaacaa gccatgactc
     1321 aggatggaga aatttactat ataaaccata agaacaagac cacctcttgg ctagacccaa
     1381 ggcttgaccc tcgttttgcc atgaaccaga gaatcagtca gagtgctcca gtgaaacagc
     1441 caccacccct ggctccccag agcccacagg gaggcgtcat gggtggcagc aactccaacc
     1501 agcagcaaca gatgcgactg cagcaactgc agatggagaa ggagaggctg cggctgaaac
     1561 agcaagaact gcttcggcag gcaatgcgga atatcaatcc cagcacagca aattctccaa
     1621 aatgtcagga gttagccctg cgtagccagt taccaacact ggagcaggat ggtgggactc
     1681 aaaatccagt gtcttctccc gggatgtctc aggaattgag aacaatgacg accaatagct
     1741 cagatccttt ccttaacagt ggcacctatc actctcgaga tgagagtaca gacagtggac
     1801 taagcatgag cagctacagt gtccctcgaa ccccagatga cttcctgaac agtgtggatg
     1861 agatggatac aggtgatact atcaaccaaa gcaccctgcc ctcacagcag aaccgtttcc
     1921 cagactacct tgaagccatt cctgggacaa atgtggacct tggaacactg gaaggagatg
     1981 gaatgaacat agaaggagag gagctgatgc caagtctgca ggaagctttg agttctgaca
     2041 tccttaatga catggagtct gttttggctg ccaccaagct agataaagaa agctttctta
     2101 catggttata gacccagctt tcttgtacaa agtggtgatc tagctaaaac gcggcctcga
     2161 atcgaattcc gccccccccc cntctccctc ccccccccct aacgttactg gccgaagccg
     2221 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     2281 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     2341 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     2401 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     2461 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     2521 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     2581 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     2641 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     2701 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
     2761 caaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     2821 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     2881 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     2941 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     3001 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3061 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3121 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3181 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     3241 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     3301 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     3361 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     3421 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     3481 agggcagcgg agagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg
     3541 gacccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     3601 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     3661 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     3721 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     3781 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     3841 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     3901 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     3961 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     4021 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     4081 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     4141 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat
     4201 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     4261 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     4321 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     4381 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     4441 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     4501 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     4561 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4621 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4681 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     4741 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4801 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4861 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     4921 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     4981 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5041 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     5101 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5161 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5221 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5281 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5341 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5401 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5461 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5521 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5581 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5641 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5701 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5761 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5821 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5881 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5941 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6001 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6061 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6121 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6181 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6241 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6301 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6361 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6421 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6481 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6541 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6601 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6661 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6721 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6781 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6841 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6901 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6961 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7021 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7081 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7141 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7201 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7261 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7321 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7381 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7441 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7501 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     7561 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     7621 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     7681 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     7741 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     7801 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     7861 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     7921 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     7981 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     8041 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     8101 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     8161 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     8221 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     8281 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     8341 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     8401 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     8461 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     8521 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     8581 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     8641 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     8701 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     8761 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     8821 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     8881 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     8941 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     9001 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     9061 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     9121 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     9181 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     9241 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     9301 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     9361 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     9421 accattatcg tttcagaccc acctcccaac cccgagggga caattctcga cctcgagaca
     9481 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     9541 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     9601 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac tttggccgcg
     9661 gctcgag
//
