LOCUS       sequence-375167-        8246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ddFP-B3-inserted mouse N-cadherin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8246)
  AUTHORS   Kanadome T, Hayashi K, Seto Y, Eiraku M, Nakajima K, Nagai T, 
            Matsuda T
  TITLE     Development of intensiometric indicators for visualizing N-cadherin 
            interaction across cells.
  JOURNAL   Commun Biol. 2022 Oct 7;5(1):1065. doi: 10.1038/s42003-022-04023-2.
  PUBMED    36207396
REFERENCE   2  (bases 1 to 8246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-10-7"; volume: "5(1):1065. doi"; pages: " 
            10.1038/s42003-022-04023-2"
COMMENT     Sequence Label: pCAG-NCad-B3
FEATURES             Location/Qualifiers
     source          1..8246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        57..436
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          684..1700
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1624..1646
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1708..1727
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(5320..5339)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5385..5440
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5439..5458)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5801..5817)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5801..5817)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5814..5836)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5825..5841
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5849..5855,5856..5873,5874..5879))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5894..5915
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5974..6169
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6020..6155
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6175..6309
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6212..6231)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6266..6285
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6377..6394)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6548..7136)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6628..6647)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7307..8098,8099..8167))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7930..7949
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8168..8246,1..26))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgaca
       61 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      121 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      181 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      241 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      301 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      361 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      421 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
      481 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
      541 ggcggggggg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
      601 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
      661 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
      721 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
      781 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
      841 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg
      901 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg
      961 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg
     1021 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga
     1081 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg
     1141 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg
     1201 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
     1261 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc
     1321 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
     1381 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga
     1441 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg
     1501 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
     1561 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg
     1621 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
     1681 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     1741 ttggcaaaga attcaccatg tgccggatag cgggagcgcc gcggaccctg ctgccgcttc
     1801 tggcggcctt gcttcaggcg tctgtggagg cttctggtga aattgcatta tgcaagactg
     1861 gatttcctga agatgtttac agcgcagtct taccgaagga tgtgcacgaa ggacagcccc
     1921 ttctcaatgt gaaattcagc aactgcaata gaaaaaggaa agttcagtat gaaagcagcg
     1981 agccagcaga tttcaaggtg gacgaggacg gcacggtgta tgctgtgaga agcttccctc
     2041 tcactgcaga gcaggcaaag ttcctgatat atgcccaaga caaagaaacc caggaaaagt
     2101 ggcaggtagc tgtaaacctg agccgggagc caaccctgac tgaggagcct atgaaggaac
     2161 cacatgaaat tgaagaaata gtattcccta gacaacttgc caagcacagt ggagctctac
     2221 aaaggcagaa gagagactgg gtcatcccgc caatcaactt gccagaaaac tccagaggac
     2281 cctttcctca agagcttgtc agaatcaggt ctgatagaga taaaaacctt tccctgagat
     2341 acagcgtcac tgggccagga gctgaccagc ctccaacggg catcttcatt atcaacccca
     2401 tctcaggaca gctgtcagtc acaaagcctc tggatcgaga gctgatagcc cggtttcact
     2461 tgagagcaca tgcagtggac atcaatggca atcaagtgga gaaccccatt gacattgtca
     2521 tcaatgttat tgacatgaat gataacagac ctgagtttct gcaccaggtt tggaatgggt
     2581 ctgttccaga gggatcaaag cctgggacgt atgtgatgac ggtcactgcc attgatgcgg
     2641 atgatccaaa tgccctgaat ggaatgctgc ggtacaggat cctgggctca gcaaccatca
     2701 aagaatttat gaggttcaaa gtacgcatgg agggtagtat gaacgggcat gagttcgaaa
     2761 ttgaaggaga aggggaaggc cgcccctatg aggggaccca aaccgccaaa cttaaagtga
     2821 caaagggagg tccacttcct tttgcctggg atatattgag cccacagttc atgtacggaa
     2881 gcgaagccta tgtacgacat cctgcagaca taccagatta caagaagctg ccctttccag
     2941 aaggctttaa gtgggaaaga gtgatgaact ttgaagatgg cggcttggta acagtgactc
     3001 aggacagcag tctccaagat ggaactttga tctgtaaggt caaaatgagg ggtactaact
     3061 ttccccccga tgggcccgtc atgcaaaaaa agacaatggg ctgggaagcc tcaaccgaga
     3121 tgttgtaccc agaagatgga gtgctcaagg gacattctta tcaggcattg aagctgaaag
     3181 atggcggaca ttacttggtt gagtttgaga ctatctatat ggccaagaag cccgtacaac
     3241 ttcctggaga ttactgcgta gacaccaaac tggatataac ctctcataac gaggattaca
     3301 ccattgtcga gcagtacggc cgcagtgaag gtagacatag acttggaatg gatgagcgat
     3361 ataagggctc agctagccag atcctgtccc aggcgcccag cacaccttca cccaacatgt
     3421 ttacaatcaa caatgagact ggggacatca tcactgtggc agctggtctg gatcgagaga
     3481 aagtgcaaca gtatacgtta ataattcaag ccacagacat ggaaggcaat cccacttatg
     3541 gcctttcaaa cacagccaca gccgtcatca cggtgacaga tgtcaatgac aatcctccag
     3601 agtttactgc catgactttc tacggagaag tccctgagaa cagggtggac gtcattgtag
     3661 ccaacctaac tgtcacggac aaagatcagc cccacacgcc ggcctggaat gcggcataca
     3721 gaatcagtgg tggagaccct acaggaaggt ttgccatcct gacagacccc aacagcaatg
     3781 atgggctagt cacagtggta aaaccaattg actttgaaac gaataggatg tttgtcctta
     3841 ctgttgctgc agaaaaccaa gtgccattag ctaaaggcat tcagcaccca cctcagtcga
     3901 cagccactgt gtctgtgaca gttattgatg tcaatgaaaa tccttatttt gccccaaatc
     3961 ctaaaatcat tcgccaagag gaaggcctcc acgcaggtac catgctgacc acgctcactg
     4021 ctcaggaccc cgatcgatat atgcaacaga atatcagata cacaaaattg tctgatcctg
     4081 ccaactggct gaaaatagac cccgtgaatg ggcagatcac tactattgcc gttttggaca
     4141 gagaatcgcc aaatgtaaaa aacaacatct ataatgctac cttccttgct tctgacaatg
     4201 gaatcccgcc tatgagtggg acaggaacac tgcaaatcta tttacttgat atcaatgaca
     4261 acgcccctca ggtgttacct caagaggcgg agacctgtga aactccagaa cccaactcaa
     4321 ttaacatcac agcacttgat tatgacatag acccaaacgc cgggccgttc gcgtttgatc
     4381 ttcccttatc tccagtgact attaaaagaa actggaccat caaccggctt aatggtgatt
     4441 ttgctcagct caatttaaag ataaaatttt tggaagctgg tatctatgaa gttcccatca
     4501 ttatcacaga ttcagggaat ccccccaagt ccaacatttc catcctgcgt gtgaaagttt
     4561 gtcagtgtga ctccaatgga gactgcacgg acgtggacag gatcgtgggt gcagggcttg
     4621 gcacgggcgc catcatcgct atccttctgt gtatcatcat cctgctgatc cttgttctca
     4681 tgtttgtggt atggatgaaa cggcgggata aagagcgcca agccaagcag cttttaattg
     4741 acccagaaga tgatgtaaga gataatatat tgaaatatga tgaagaaggt ggaggagaag
     4801 aagaccagga ctatgacttg agccagctcc agcaaccaga tactgtggag cctgatgcca
     4861 tcaagcccgt gggaatcaga cggctagacg agaggcctat ccatgctgag ccacagtacc
     4921 cagtccgatc cgcagcccca caccctgggg atattgggga cttcattaat gagggcctta
     4981 aagctgctga caacgacccc acggcgccac cgtatgactc cctcttagtc tttgactacg
     5041 agggcagcgg ctccacggct ggctccttga gctccctcaa ctcctccagt agcggtgggg
     5101 accaggacta tgactacctg aatgactggg gaccccgctt caagaaactg gcggacatgt
     5161 acggcggtgg tgacgacaag ctttacccat acgacgtccc agactaccgg taactcgagg
     5221 cggccgcttt tttccttaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc
     5281 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
     5341 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     5401 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     5461 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     5521 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     5581 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     5641 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     5701 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     5761 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     5821 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     5881 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     5941 tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag caaccatagt
     6001 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6061 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     6121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg
     6181 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6241 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6301 gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6361 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6421 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6481 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6541 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6601 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6661 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     6721 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     6781 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     6841 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6901 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6961 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7021 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7081 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7141 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7201 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7261 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7321 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7381 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7441 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7501 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7561 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7621 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     7681 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     7741 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     7801 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     7861 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     7921 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7981 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8041 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8101 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8161 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8221 gcggatacat atttgaatgt atttag
//
