LOCUS       sequence-375168-        8419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mVenus-inserted mouse N-cadherin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Kanadome T, Hayashi K, Seto Y, Eiraku M, Nakajima K, Nagai T, 
            Matsuda T
  TITLE     Development of intensiometric indicators for visualizing N-cadherin 
            interaction across cells.
  JOURNAL   Commun Biol. 2022 Oct 7;5(1):1065. doi: 10.1038/s42003-022-04023-2.
  PUBMED    36207396
REFERENCE   2  (bases 1 to 8419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-10-7"; volume: "5(1):1065. doi"; pages: " 
            10.1038/s42003-022-04023-2"
COMMENT     Sequence Label: pCAG-NCad-V
FEATURES             Location/Qualifiers
     source          1..8419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        89..468
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          715..1731
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1655..1677
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1739..1758
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(2857..2859,2860..3570)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2899..2920)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3160..3179)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3507..3528
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5525..5544)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5590..5645
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5644..5663)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6006..6022)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6006..6022)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6019..6041)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6030..6046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6054..6060,6061..6078,6079..6084))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6099..6120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6179..6374
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6225..6360
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6380..6514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6417..6436)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6471..6490
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6582..6599)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6753..7341)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6833..6852)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7512..8303,8304..8372))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8135..8154
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8373..8419,1..58))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
       61 catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta ttaatagtaa
      121 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      181 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      241 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      301 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      361 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      421 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc
      481 cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta
      541 tttatttttt aattattttg tgcagcgatg ggggcggggg gggggnnngg gcggggcggg
      601 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
      661 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
      721 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
      781 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
      841 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
      901 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
      961 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
     1021 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
     1081 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
     1141 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
     1201 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
     1261 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
     1321 cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt
     1381 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
     1441 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
     1501 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1561 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
     1621 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
     1681 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
     1741 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcggggg aggggagggg
     1801 aagggaaggg gtggccactg cccggagccg actccgcgct gctgttggtg ccgctgccgc
     1861 ttctgctgcc tctgctgccg ccgccgccgc ctccggctcc tcgctcggcc cctctccgcc
     1921 tccatgtgcc ggatagcggg agcgccgcgg accctgctgc cgcttctggc ggccttgctt
     1981 caggcgtctg tggaggcttc tggtgaaatt gcattatgca agactggatt tcctgaagat
     2041 gtttacagcg cagtcttacc gaaggatgtg cacgaaggac agccccttct caatgtgaaa
     2101 ttcagcaact gcaatagaaa aaggaaagtt cagtatgaaa gcagcgagcc agcagatttc
     2161 aaggtggacg aggacggcac ggtgtatgct gtgagaagct tccctctcac tgcagagcag
     2221 gcaaagttcc tgatatatgc ccaagacaaa gaaacccagg aaaagtggca ggtagctgta
     2281 aacctgagcc gggagccaac cctgactgag gagcctatga aggaaccaca tgaaattgaa
     2341 gaaatagtat tccctagaca acttgccaag cacagtggag ctctacaaag gcagaagaga
     2401 gactgggtca tcccgccaat caacttgcca gaaaactcca gaggaccctt tcctcaagag
     2461 cttgtcagaa tcaggtctga tagagataaa aacctttccc tgagatacag cgtcactggg
     2521 ccaggagctg accagcctcc aacgggcatc ttcattatca accccatctc aggacagctg
     2581 tcagtcacaa agcctctgga tcgagagctg atagcccggt ttcacttgag agcacatgca
     2641 gtggacatca atggcaatca agtggagaac cccattgaca ttgtcatcaa tgttattgac
     2701 atgaatgata acagacctga gtttctgcac caggtttgga atgggtctgt tccagaggga
     2761 tcaaagcctg ggacgtatgt gatgacggtc actgccattg atgcggatga tccaaatgcc
     2821 ctgaatggaa tgctgcggta caggatcctg ggctcagtga gcaagggcga ggagctgttc
     2881 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     2941 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc
     3001 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg
     3061 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     3121 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     3181 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     3241 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     3301 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
     3361 cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
     3421 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc
     3481 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     3541 atcactctcg gcatggacga gctgtacaag ggctcagcta gccagatcct gtcccaggcg
     3601 cccagcacac cttcacccaa catgtttaca atcaacaatg agactgggga catcatcact
     3661 gtggcagctg gtctggatcg agagaaagtg caacagtata cgttaataat tcaagccaca
     3721 gacatggaag gcaatcccac ttatggcctt tcaaacacag ccacagccgt catcacggtg
     3781 acagatgtca atgacaatcc tccagagttt actgccatga ctttctacgg agaagtccct
     3841 gagaacaggg tggacgtcat tgtagccaac ctaactgtca cggacaaaga tcagccccac
     3901 acgccggcct ggaatgcggc atacagaatc agtggtggag accctacagg aaggtttgcc
     3961 atcctgacag accccaacag caatgatggg ctagtcacag tggtaaaacc aattgacttt
     4021 gaaacgaata ggatgtttgt ccttactgtt gctgcagaaa accaagtgcc attagctaaa
     4081 ggcattcagc acccacctca gtcgacagcc actgtgtctg tgacagttat tgatgtcaat
     4141 gaaaatcctt attttgcccc aaatcctaaa atcattcgcc aagaggaagg cctccacgca
     4201 ggtaccatgc tgaccacgct cactgctcag gaccccgatc gatatatgca acagaatatc
     4261 agatacacaa aattgtctga tcctgccaac tggctgaaaa tagaccccgt gaatgggcag
     4321 atcactacta ttgccgtttt ggacagagaa tcgccaaatg taaaaaacaa catctataat
     4381 gctaccttcc ttgcttctga caatggaatc ccgcctatga gtgggacagg aacactgcaa
     4441 atctatttac ttgatatcaa tgacaacgcc cctcaggtgt tacctcaaga ggcggagacc
     4501 tgtgaaactc cagaacccaa ctcaattaac atcacagcac ttgattatga catagaccca
     4561 aacgccgggc cgttcgcgtt tgatcttccc ttatctccag tgactattaa aagaaactgg
     4621 accatcaacc ggcttaatgg tgattttgct cagctcaatt taaagataaa atttttggaa
     4681 gctggtatct atgaagttcc catcattatc acagattcag ggaatccccc caagtccaac
     4741 atttccatcc tgcgtgtgaa agtttgtcag tgtgactcca atggagactg cacggacgtg
     4801 gacaggatcg tgggtgcagg gcttggcacg ggcgccatca tcgctatcct tctgtgtatc
     4861 atcatcctgc tgatccttgt tctcatgttt gtggtatgga tgaaacggcg ggataaagag
     4921 cgccaagcca agcagctttt aattgaccca gaagatgatg taagagataa tatattgaaa
     4981 tatgatgaag aaggtggagg agaagaagac caggactatg acttgagcca gctccagcaa
     5041 ccagatactg tggagcctga tgccatcaag cccgtgggaa tcagacggct agacgagagg
     5101 cctatccatg ctgagccaca gtacccagtc cgatccgcag ccccacaccc tggggatatt
     5161 ggggacttca ttaatgaggg ccttaaagct gctgacaacg accccacggc gccaccgtat
     5221 gactccctct tagtctttga ctacgagggc agcggctcca cggctggctc cttgagctcc
     5281 ctcaactcct ccagtagcgg tggggaccag gactatgact acctgaatga ctggggaccc
     5341 cgcttcaaga aactggcgga catgtacggc ggtggtgacg acaagcttta cccatacgac
     5401 gtcccagact accggtaact cgaggcggcc gcttttttcc ttaattcact cctcaggtgc
     5461 aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact
     5521 gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga
     5581 cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc
     5641 tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg
     5701 tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa
     5761 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct
     5821 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc
     5881 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag
     5941 tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag cttggcgtaa
     6001 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6061 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6121 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc
     6181 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     6241 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6301 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6361 taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa
     6421 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6481 caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc
     6541 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6601 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6661 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6721 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6781 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6841 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6901 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6961 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7021 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7081 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7141 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7201 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7261 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7321 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7381 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7441 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7501 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7561 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7621 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     7681 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     7741 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     7801 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     7861 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     7921 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     7981 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8041 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8101 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8161 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8221 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8281 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     8341 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     8401 atttatcagg gttattgtc
//
