LOCUS       sequence-375169-        8264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ddGFP-A-inserted mouse N-cadherin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8264)
  AUTHORS   Kanadome T, Hayashi K, Seto Y, Eiraku M, Nakajima K, Nagai T, 
            Matsuda T
  TITLE     Development of intensiometric indicators for visualizing N-cadherin 
            interaction across cells.
  JOURNAL   Commun Biol. 2022 Oct 7;5(1):1065. doi: 10.1038/s42003-022-04023-2.
  PUBMED    36207396
REFERENCE   2  (bases 1 to 8264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-10-7"; volume: "5(1):1065. doi"; pages: " 
            10.1038/s42003-022-04023-2"
COMMENT     Sequence Label: pCAG-NCad-GA
FEATURES             Location/Qualifiers
     source          1..8264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          123..1139
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1063..1085
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1147..1166
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(4789..4808)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4854..4909
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4908..4927)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5270..5286)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5270..5286)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5283..5305)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5294..5310
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5318..5324,5325..5342,5343..5348))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5363..5384
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5443..5638
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5489..5624
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5644..5778
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5681..5700)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5735..5754
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5846..5863)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6017..6605)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6097..6116)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6776..7567,7568..7636))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7399..7418
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7637..7741)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7772..8151
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
       61 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      121 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      181 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      241 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      301 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      361 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      421 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
      481 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
      541 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
      601 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
      661 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
      721 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
      781 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
      841 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
      901 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
      961 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1021 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1081 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1141 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcaccatgt
     1201 gccggatagc gggagcgccg cggaccctgc tgccgcttct ggcggccttg cttcaggcgt
     1261 ctgtggaggc ttctggtgaa attgcattat gcaagactgg atttcctgaa gatgtttaca
     1321 gcgcagtctt accgaaggat gtgcacgaag gacagcccct tctcaatgtg aaattcagca
     1381 actgcaatag aaaaaggaaa gttcagtatg aaagcagcga gccagcagat ttcaaggtgg
     1441 acgaggacgg cacggtgtat gctgtgagaa gcttccctct cactgcagag caggcaaagt
     1501 tcctgatata tgcccaagac aaagaaaccc aggaaaagtg gcaggtagct gtaaacctga
     1561 gccgggagcc aaccctgact gaggagccta tgaaggaacc acatgaaatt gaagaaatag
     1621 tattccctag acaacttgcc aagcacagtg gagctctaca aaggcagaag agagactggg
     1681 tcatcccgcc aatcaacttg ccagaaaact ccagaggacc ctttcctcaa gagcttgtca
     1741 gaatcaggtc tgatagagat aaaaaccttt ccctgagata cagcgtcact gggccaggag
     1801 ctgaccagcc tccaacgggc atcttcatta tcaaccccat ctcaggacag ctgtcagtca
     1861 caaagcctct ggatcgagag ctgatagccc ggtttcactt gagagcacat gcagtggaca
     1921 tcaatggcaa tcaagtggag aaccccattg acattgtcat caatgttatt gacatgaatg
     1981 ataacagacc tgagtttctg caccaggttt ggaatgggtc tgttccagag ggatcaaagc
     2041 ctgggacgta tgtgatgacg gtcactgcca ttgatgcgga tgatccaaat gccctgaatg
     2101 gaatgctgcg gtacaggatc ctgggctcag ttagtaagag cgaggaggta atcaaggagt
     2161 ttatgaggtt taaggtcagg ctcgagggca gcatgaatgg gcatgagttt gagatagagg
     2221 gtgaagggga aggcagacca tatgagggta ctcaaacagc caagctcaaa gtaaccaagg
     2281 gcggtccact ccctttcgca tgggatattt tgagtcccct tattatgtat ggttccaaaa
     2341 tgtatgtgaa gcaccctgca gatgtgcctg attatatgaa actgtcattc cctgagggtt
     2401 tcaagtggga aagagtgatg cattttgaag atggtggact cgtcacagct acacaggaca
     2461 gttccttgca agatggtaca cttatttaca aagtaaagat gcggggtaca aacttccctc
     2521 cagatggtcc cgttatgcag aagaagacac tcggttggga ctacgcaaca gagcgactct
     2581 atcccgagga gggcgttctc aaaggtgaac tgctgggacg actgaaactc aaggacggcg
     2641 gactgaatct ggtggaaagt aagaccatat acatggctaa aaaacccgta caactcccag
     2701 gatactattt tgtcgacaca aagctggata ttacctccca taatgaggat tacaccattg
     2761 tagagcaata cgagaggagt gaaggccggc atcatctggg acatggtaca ggctctactg
     2821 gatctggcag tagcggctca gctagccaga tcctgtccca ggcgcccagc acaccttcac
     2881 ccaacatgtt tacaatcaac aatgagactg gggacatcat cactgtggca gctggtctgg
     2941 atcgagagaa agtgcaacag tatacgttaa taattcaagc cacagacatg gaaggcaatc
     3001 ccacttatgg cctttcaaac acagccacag ccgtcatcac ggtgacagat gtcaatgaca
     3061 atcctccaga gtttactgcc atgactttct acggagaagt ccctgagaac agggtggacg
     3121 tcattgtagc caacctaact gtcacggaca aagatcagcc ccacacgccg gcctggaatg
     3181 cggcatacag aatcagtggt ggagacccta caggaaggtt tgccatcctg acagacccca
     3241 acagcaatga tgggctagtc acagtggtaa aaccaattga ctttgaaacg aataggatgt
     3301 ttgtccttac tgttgctgca gaaaaccaag tgccattagc taaaggcatt cagcacccac
     3361 ctcagtcgac agccactgtg tctgtgacag ttattgatgt caatgaaaat ccttattttg
     3421 ccccaaatcc taaaatcatt cgccaagagg aaggcctcca cgcaggtacc atgctgacca
     3481 cgctcactgc tcaggacccc gatcgatata tgcaacagaa tatcagatac acaaaattgt
     3541 ctgatcctgc caactggctg aaaatagacc ccgtgaatgg gcagatcact actattgccg
     3601 ttttggacag agaatcgcca aatgtaaaaa acaacatcta taatgctacc ttccttgctt
     3661 ctgacaatgg aatcccgcct atgagtggga caggaacact gcaaatctat ttacttgata
     3721 tcaatgacaa cgcccctcag gtgttacctc aagaggcgga gacctgtgaa actccagaac
     3781 ccaactcaat taacatcaca gcacttgatt atgacataga cccaaacgcc gggccgttcg
     3841 cgtttgatct tcccttatct ccagtgacta ttaaaagaaa ctggaccatc aaccggctta
     3901 atggtgattt tgctcagctc aatttaaaga taaaattttt ggaagctggt atctatgaag
     3961 ttcccatcat tatcacagat tcagggaatc cccccaagtc caacatttcc atcctgcgtg
     4021 tgaaagtttg tcagtgtgac tccaatggag actgcacgga cgtggacagg atcgtgggtg
     4081 cagggcttgg cacgggcgcc atcatcgcta tccttctgtg tatcatcatc ctgctgatcc
     4141 ttgttctcat gtttgtggta tggatgaaac ggcgggataa agagcgccaa gccaagcagc
     4201 ttttaattga cccagaagat gatgtaagag ataatatatt gaaatatgat gaagaaggtg
     4261 gaggagaaga agaccaggac tatgacttga gccagctcca gcaaccagat actgtggagc
     4321 ctgatgccat caagcccgtg ggaatcagac ggctagacga gaggcctatc catgctgagc
     4381 cacagtaccc agtccgatcc gcagccccac accctgggga tattggggac ttcattaatg
     4441 agggccttaa agctgctgac aacgacccca cggcgccacc gtatgactcc ctcttagtct
     4501 ttgactacga gggcagcggc tccacggctg gctccttgag ctccctcaac tcctccagta
     4561 gcggtgggga ccaggactat gactacctga atgactgggg accccgcttc aagaaactgg
     4621 cggacatgta cggcggtggt gacgacaagc tttacccata cgacgtccca gactaccggt
     4681 aactcgaggc ggccgctttt ttccttaatt cactcctcag gtgcaggctg cctatcagaa
     4741 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc
     4801 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
     4861 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     4921 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
     4981 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
     5041 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
     5101 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
     5161 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
     5221 tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt
     5281 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     5341 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     5401 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc
     5461 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5521 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     5581 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     5641 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     5701 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     5761 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     5821 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     5881 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     5941 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6001 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6061 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6121 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6181 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6241 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6301 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6361 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6421 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     6481 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6541 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6601 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6661 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6721 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6781 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6841 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6901 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6961 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7021 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7081 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7141 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7201 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7261 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7321 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7381 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7441 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7501 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7561 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7621 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7681 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7741 gcacatttcc ccgaaaagtg ccacctgggt cgacattgat tattgactag ttattaatag
     7801 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     7861 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     7921 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     7981 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     8041 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     8101 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg
     8161 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat
     8221 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggg
//
