LOCUS       sequence-37517-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  TITLE     TGF Beta Type II
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRK5-TGF beta Type II receptor Flag
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(59..82)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(505..516)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ataagtgcat aacagttggg ccatggcggc caagcttctg caggtcgact ctagactatt
       61 tatcgtcatc gtctttgtag tccatggatc catcttcagg aatcttctcc tccgagcagc
      121 tcctccccga gagcctgtcc agatgctcca gctcactgaa gcgttctgcc acacactggg
      181 ctgtgagacg ggcctctggg tcgtggtccc agcactcagt caacgtctca cacaccatct
      241 ggatgccctg gtggttgagc cagaagctgg gaatttctgg tcgccctcga tctctcaaca
      301 cgttgtcctt catgctttcg acacaggggt gctcccgcac cttggaacca aatggaggct
      361 cataatcttt tacttctccc actgcattac agcgagatgt catttcccag agcaccagag
      421 ccatggagta gacatcggtc tgcttgaagg actcagcatt ctccaaattc atcctggatt
      481 ctaggacttc tggagccatg tatcttgcag ttcccacctg cccactgtta gccaggtcat
      541 ccacagacag agtagggtcc agacgcaggg aaagcccaaa gtcacacagg cagcaggtta
      601 ggtcgttctt cacgaggata ttggagctct tgaggtccct gtgcacgatg ggcatcttgg
      661 gcctcccaca tggagtgtga tcactgtgga ggtgagcaat cccccgggcg agggagctgc
      721 ccagcttgcg caggtcctcc cagctgatga
//
