LOCUS       sequence-375173-        3555 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses K2P10.1 with C-terminal StrepTagII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Zhu Y, Schrecke S, Tang S, Odenkirk MT, Walker T, Stover L, Lyu J, 
            Zhang T, Russell D, Baker ES, Yan X, Laganowsky A
  TITLE     Cupric Ions Selectively Modulate TRAAK-Phosphatidylserine 
            Interactions.
  JOURNAL   J Am Chem Soc. 2022 Apr 27;144(16):7048-7053. doi: 
            10.1021/jacs.2c00612. Epub 2022 Apr 14.
  PUBMED    35421309
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.2c00612"; journalName: "J Am Chem Soc"; date: 
            "2022-04-27- 27"; volume: "144"; issue: "16"; pages: "7048-7053"
COMMENT     Sequence Label: pACE-K2P10.1-StrepTagII
FEATURES             Location/Qualifiers
     source          1..3555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(620..625,626..642,643..648))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(719..752)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        829..920
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     845..862
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     regulatory      925..934
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1780..1803
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    1830..1964
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1867..1886)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1921..1940
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2085..2250
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2440..3028
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2929..2948
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  3163..3386
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(join(3453..3555,1..431))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 acgtagatca cataagcacc aagcgcgttg gcctcatgct tgaggagatt gatgagcgcg
       61 gtggcaatgc cctgcctccg gtgctcgccg gagactgcga gatcatagat atagatctca
      121 ctacgcggct gctcaaactt gggcagaacg taagccgcga gagcgccaac aaccgcttct
      181 tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga gcaagttccc gaggtaatcg
      241 gagtccggct gatgttggga gtaggtggct acatcgccga actcacgacc gaaaagatca
      301 agagcagccc gcatggattt gacttggtca gggccgagcc tacatgtgcg aatgatgccc
      361 atacttgagc cacctaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
      421 ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg
      481 ctgcttggat gcccgaggca tagactgtac aaaaaaacag tcataacaag ccatgaaaac
      541 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
      601 catacgctac ttgcattaca gtttacgaac cgaacaggct tatgtcaact gggttcgtgc
      661 cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgccat
      721 aacttcgtat agcatacatt atacgaagtt atctgtaact ataacggtcc taaggtagcg
      781 agtttaaaca ctagtatcga ttcgcgacct actccggaat attaatagat catggagata
      841 attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg
      901 taataaaaaa acctataaat aaccgccacc atggagggag cgtcccaagg aggcctgcaa
      961 acagtgatga agtggaagac cgtagttgcc atatttgtcg tcgtggtcgt atatctggta
     1021 accggaggac ttgtattccg tgctctggaa cagccgtttg agtccagtca gaaaaacact
     1081 attgccctgg agaaggcgga attcctgaga gaccacgtat gcgtatcgcc tcaggagctg
     1141 gaaaccctta ttcagcacgc actggacgcc gacaacgcag gagtctcacc aattggacaa
     1201 agttcgaata acagtagtca ctgggacctg ggcagtgctt tcttcttcgc aggcacggta
     1261 atcactacca taggctatgg aaatatagct ccttcaacag agggtggtaa gattttttgt
     1321 atcctttatg ctatattcgg tatcccactt ttcggttttc ttttggctgg aatcggagat
     1381 caactgggaa ctatctttgg taaatccatc gcccgtgttg agaaagtttt taggaaaaag
     1441 caagtctctc agaccaaaat aagagttatc tccacgattc tgtttatact tgccggttgt
     1501 attgttttcg tgacaatacc agcagtgatc ttcaagtaca tagagggctg gacggctctg
     1561 gagagcatat atttcgtcgt agttactctt acaactgtag gatttggtga cttcgttgcc
     1621 ggtggtaatg caggaatcaa ctatagggag tggtacaagc cgcttgtgtg gttctggatc
     1681 ctggtaggcc tcgcatattt cgcagccgta ctgtcaatga taggagattg gctccgcgta
     1741 ctttccaaaa aaacaaagga ggaagtgggc gaagcttcct ggtcacatcc acagtttgag
     1801 aaataataat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     1861 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     1921 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgatca ctgcttgagc
     1981 ctagaagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
     2041 gagcaataac tatcataacc cctagggtat acccatctaa ttggaaccag ataagtgaaa
     2101 tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca
     2161 gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct
     2221 cccagttccc aactattttg tccgcccaca accggttgac ttgggtcaac tgtcagacca
     2281 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     2341 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     2401 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2461 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2521 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2581 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2641 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2701 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2761 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2821 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2881 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2941 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3001 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3061 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attgacttgg
     3121 gtcgctcttc ctgtggatgc gcagatgccc tgcgtaagcg ggtgtgggcg gacaataaag
     3181 tcttaaactg aacaaaatag atctaaacta tgacaataaa gtcttaaact agacagaata
     3241 gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc atactggact tttgttatgg
     3301 ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg
     3361 gccaagggca tgtaaagact atattcgcgg cgttgtgaca atttaccgaa caactccgcg
     3421 gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca
     3481 aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca
     3541 ccgtaatctg cttgc
//
