LOCUS       sequence-37520-e         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid 
            #8452) with GFP instead of puromycin resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  TITLE     Viral vectors.
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMKO.1 GFP
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(85..105)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        90..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(428..444)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(429..445)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 ttatatatct tgtggaagga cgaaacaccg gtacgcgtac tagtactccc atatggaatt
       61 cgccagcaca gtggtcgacc ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttatcga taccgtcgac ctcgagacca tggtgagcaa gcagatcctg aagaacaccg
      481 gcctgcagga gatcatgagc ttcaaggtga acctggaggg cgtggtgaac aaccacgtgt
      541 tcaccatgga gggctgcggc aagggcaaca tcctgttcgg caaccagctg gtgcagatcc
      601 gcgtgaccaa gggcgccccc ctgcccttcg ccttcgacat cctgagcccc gccttccagt
      661 acggcaaccg caccttcacc aagtaccccg aggacatcag cgacttcttc atccagagct
      721 tccccgccgg cttcgtgtac gagcgcaccc tgcgcta
//
