LOCUS       sequence-375207-        9286 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid vector for in vitro Jurkat activation screening (for immune 
            library).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9286)
  AUTHORS   Lu Z, Ni K, Wang Y, Zhou Y, Li Y, Yan J, Song Q, Liu M, Xu Y, Yu Z, 
            Guo T, Ma L
  TITLE     An in-library ligation strategy and its application in CRISPR/Cas9 
            screening of high-order gRNA combinations.
  JOURNAL   Nucleic Acids Res. 2022 Jun 24;50(11):6575-6586. doi: 
            10.1093/nar/gkac458.
  PUBMED    35670669
REFERENCE   2  (bases 1 to 9286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2022-06-24- 24"; volume: "50"; issue: 
            "11"; pages: "6575-6586"
COMMENT     Sequence Label: T cell activation (library 1) Backbone
FEATURES             Location/Qualifiers
     source          1..9286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        156..1334
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          387..1325
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1282..1302
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1347..1943
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1347..1366)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1843..1863
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1944..1997
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1998..2693
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="VSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKA
                     VEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALK
                     LVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHR"
     misc_feature    2715..3303
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2768..2788)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3186..3197)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3375..3608
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3686..3807
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3723..3742)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3777..3796
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3847..3982
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4002..4021)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4003..4021)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4031..4048)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4031..4047)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4040..4062)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4189..4644
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4276..4295)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4486..4507
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4670..4774
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4775..4843,4844..5635)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4993..5012)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5806..6394
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6295..6314
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6548..6565
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6682..6703
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6718..6723,6724..6741,6742..6748)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6756..6772
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6761..6783
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6780..6796
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6780..6796
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6815..6835
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6817..6835
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6863..7089
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    7317..7442
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7935..8168
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8353..8397
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8575..8823
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8575..8595
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8746..8765
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    join(9186..9286,1..17)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc
       61 tcgagggggc ccgggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
      121 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      181 agcgcacatg gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgatccggtg
      241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      361 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      421 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      481 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      541 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      601 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      661 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      721 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      781 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      841 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      901 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      961 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     1021 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1081 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1141 cgtctttagg ttggggggag gggttttatg cgttggagtt tccccacact gagtgggtgg
     1201 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1261 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1321 ttcaggtgtc gtgacgtacg gccaccatga ccgagtacaa gcccacggtg cgcctcgcca
     1381 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     1441 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     1501 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     1561 ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     1621 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     1681 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggagtctcgc
     1741 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     1801 agcgcgccgg ggtgcccgcc ttcctggaaa cctccgcgcc ccgcaacctc cccttctacg
     1861 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     1921 gcatgacccg caagcccggt gccgagggca ggggaagtct gctaacatgc ggggacgtgg
     1981 aggaaaatcc cggccccgtg agcgagctga ttaaggagaa catgcacatg aagctgtaca
     2041 tggagggcac cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct
     2101 acgagggcac ccagaccatg agaatcaagg cggtcgaggg cggccctctc cccttcgcct
     2161 tcgacatcct ggctaccagc ttcatgtacg gcagcaaaac cttcatcaac cacacccagg
     2221 gcatccccga cttctttaag cagtccttcc ccgagggctt cacatgggag agagtcacca
     2281 catacgagga cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc
     2341 tcatctacaa cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgcaga
     2401 agaaaacact cggctgggag gcctccaccg aaaccctgta ccccgctgac ggcggcctgg
     2461 aaggcagagc cgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttga
     2521 aaaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctactatg
     2581 tggacagaag gctggaaaga atcaaggagg ccgacaaaga aacctacgtc gagcagcacg
     2641 aggtggctgt ggccagatac tgcgacctcc ctagcaaact ggggcacaga taaggatcca
     2701 cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     2761 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     2821 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     2881 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     2941 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     3001 ctttccccct ccctattgcc acggcggaac tcaacgccgc ctgccttgcc cgctgctgga
     3061 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     3121 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     3181 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     3241 ctcttccgcg tgttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     3301 cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     3361 agaaaagggg ggactggaag ggctaattca ctcccaacga tgacaagatc tgctttttgc
     3421 ttgtactggc tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     3481 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     3541 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     3601 ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     3661 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     3721 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3781 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     3841 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     3901 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     3961 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     4021 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     4081 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     4141 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     4201 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     4261 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     4321 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     4381 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcga tcgccctgat
     4441 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     4501 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     4561 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     4621 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4681 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4741 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4801 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4861 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4921 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4981 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5041 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5101 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5161 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5221 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5281 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5341 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5401 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5461 tgctgataaa tctggagccg gtgagcgtgg gtcccgcggt atcattgcag cactggggcc
     5521 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5581 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5641 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5701 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5761 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5821 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5881 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5941 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6001 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6061 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6121 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6181 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6241 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6301 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6361 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6421 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6481 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     6541 cgagcgcagc gagtcagtga gcgaggaagc ggatgagcgc ccaatacgca aaccgcctct
     6601 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     6661 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     6721 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     6781 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     6841 aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
     6901 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
     6961 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
     7021 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
     7081 tacataaacg gctctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7141 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7201 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7261 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     7321 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     7381 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     7441 tcagtattaa gcgggggaga attagatcgc gttgggaaaa aattcggtta aggccagggg
     7501 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     7561 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     7621 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     7681 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     7741 agcaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     7801 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     7861 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     7921 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     7981 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     8041 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     8101 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     8161 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     8221 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     8281 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     8341 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     8401 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     8461 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     8521 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cagagagggc
     8581 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt
     8641 agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
     8701 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
     8761 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
     8821 accggagacg gttgtaaaga tatctgagca cacaaaatac agaattccat gctaaaatat
     8881 tgttcctgtt ccattaactg cgctaaaaat aaacgtctct gtttgagagc tatgctggaa
     8941 acagcatagc aagttcaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     9001 cggtgctcgg aaaaaaaaga aaaaaagaaa aaaagaaaaa aatccatgac caaattggtc
     9061 catggattca cccacccctt tttatttttt tcaccgtggg caggtgaatc gatacacctg
     9121 catgggcgat tctagaaagc ttggcgtaac tagatcttga gacaaatggc agtattcatc
     9181 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     9241 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaatta
//
