LOCUS       sequence-375233-       14395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xFLAG-KPNB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14395)
  AUTHORS   Wang L, Paudel BB, McKnight RA, Janes KA
  TITLE     Nucleocytoplasmic transport of active HER2 causes fractional escape 
            from the DCIS-like state.
  JOURNAL   Nat Commun. 2023 Apr 13;14(1):2110. doi: 10.1038/s41467-023-37914-x.
  PUBMED    37055441
REFERENCE   2  (bases 1 to 14395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-13"; volume: "14(1):2110. doi"; pages: " 
            10.1038/s41467-023-37914-x"
COMMENT     Sequence Label: pSLIK 3xFLAG-KPNB1 zeo
FEATURES             Location/Qualifiers
     source          1..14395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2670..2984
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2678..2696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2714..2732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2749..2767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2892..2910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2957..2981
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2993..3058
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(5731..5755)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5805..7016
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6853..6872
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(7075..7692,7693..7698,7699..7734,7735..7737,7738..7773,
                     7774..7776,7777..7812,7813..7821)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7874..8447
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8041..8058)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8268..8287
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        8469..8516
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8535..8909
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    9083..9671
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9136..9156)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9554..9565)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9674..9690)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9675..9691)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10200..10380
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10406..10423)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10412..10636
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10682..11110
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10769..10788)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10979..11000
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11119..11139)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11124..11453
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11304..11439
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11366..11385
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11501..11548
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11567..11941
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12071..12192
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12108..12127)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12162..12181
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12241..12257)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12241..12257)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12254..12276)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12265..12281
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12289..12295,12296..12313,12314..12319))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12334..12355
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12472..12489)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12643..13231)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12723..12742)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13402..14193,14194..14262))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14025..14044
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14263..14367)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt
     2641 aaaggaacca attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt
     2701 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
     2761 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2821 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     2881 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     2941 aggcctatat aagcagagct cgtttagtga accgtcagat cgccactaga tggactacaa
     3001 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaagac
     3061 tagaatggag ctgatcacca ttctcgagaa gaccgtgtct cccgatcggc tggagctgga
     3121 agcggcgcag aagttcctgg agcgtgcggc cgtggagaac ctgcccactt tccttgtgga
     3181 actgtccaga gtgctggcaa atccaggaaa cagtcaggtt gccagagttg cagctggtct
     3241 acaaatcaag aactctttga catctaaaga tccagatatc aaggcacaat atcagcagag
     3301 gtggcttgct attgatgcta atgctcgacg agaagtcaag aactatgttt tgcagacatt
     3361 gggtacagaa acttaccggc ctagttctgc ctcacagtgt gtggctggta ttgcttgtgc
     3421 agagatccca gtaaaccagt ggccagaact cattcctcag ctggtggcca atgtcacaaa
     3481 ccccaacagc acagagcaca tgaaggagtc gacattggaa gccatcggtt atatttgcca
     3541 agatatagac ccagagcagc tacaagataa atccaatgag attctgactg ccataatcca
     3601 ggggatgagg aaagaagagc ctagtaataa tgtgaagcta gctgctacga atgcactcct
     3661 gaactcattg gagttcacca aagcaaactt tgataaagag tctgaaaggc actttattat
     3721 gcaggtggtc tgtgaagcca cacagtgtcc agatacgagg gtacgagtgg ctgctttaca
     3781 gaatctggtg aagataatgt ccttatatta tcagtacatg gagacatata tgggtcctgc
     3841 tctttttgca atcacaatcg aagcaatgaa aagtgacatt gatgaggtgg ctttacaagg
     3901 gatagaattc tggtccaatg tctgtgatga ggaaatggat ttggccattg aagcttcaga
     3961 ggcagcagaa caaggacggc cccctgagca caccagcaag ttttatgcga agggagcact
     4021 acagtatctg gttccaatcc tcacacagac actaactaaa caggacgaaa atgatgatga
     4081 cgatgactgg aacccctgca aagcagcagg ggtgtgcctc atgcttctgg ccacctgctg
     4141 tgaagatgac attgtcccac atgtcctccc cttcattaaa gaacacatca agaacccaga
     4201 ttggcggtac cgggatgcag cagtgatggc ttttggttgt atcttggaag gaccagagcc
     4261 cagtcagctc aaaccactag ttatacaggc tatgcccacc ctaatagaat taatgaaaga
     4321 ccccagtgta gttgttcgag atacagctgc atggactgta ggcagaattt gtgagctgct
     4381 tcctgaagct gccatcaatg atgtctactt ggctcccctg ctacagtgtc tgattgaggg
     4441 tctcagtgct gaacccagag tggcttcaaa tgtgtgctgg gctttctcca gtctggctga
     4501 agctgcttat gaagctgcag acgttgctga tgatcaggaa gaaccagcta cttactgctt
     4561 atcttcttca tttgaactca tagttcagaa gctcctagag actacagaca gacctgatgg
     4621 acaccagaac aacctgagaa gttctgcata tgaatctctg atggaaattg tgaaaaacag
     4681 tgccaaggat tgttatcctg ctgtccagaa aacgactttg gtcatcatgg aacgactgca
     4741 acaggttctt cagatggagt cacatatcca gagcacatcc gatagaatcc agttcaatga
     4801 ccttcagtct ttactctgtg caactcttca gaatgttctt cggaaagtgc aacatcaaga
     4861 tgctttgcag atctctgatg tggttatggc ctccctgtta aggatgttcc aaagcacagc
     4921 tgggtctggg ggagtacaag aggatgccct gatggcagtt agcacactgg tggaagtgtt
     4981 gggtggtgaa ttcctcaagt acatggaggc ctttaaaccc ttcctgggca ttggattaaa
     5041 aaattatgct gaataccagg tttgtttggc agctgtgggc ttagtgggag acttgtgccg
     5101 tgccctgcaa tccaacatca tacctttctg tgacgaggtg atgcagctgc ttctggaaaa
     5161 tttggggaat gagaacgtcc acaggtctgt gaagccgcag attctgtcag tgtttggtga
     5221 tattgccctt gctattggag gagagtttaa aaaatactta gaggttgtat tgaatactct
     5281 tcagcaggcc tcccaagccc aggtggacaa gtcagactat gacatggtgg attatctgaa
     5341 tgagctaagg gaaagctgct tggaagccta tactggaatc gtccagggat taaaggggga
     5401 tcaggagaac gtacacccgg atgtgatgct ggtacaaccc agagtagaat ttattctgtc
     5461 tttcattgac cacattgctg gagatgagga tcacacagat ggagtagtag cttgtgctgc
     5521 tggactaata ggggacttat gtacagcatt tgggaaggat gtactgaaat tagtagaagc
     5581 taggccaatg atccatgaat tgttaactga agggcggaga tcgaagacta acaaagcaaa
     5641 aacccttgct acatgggcaa caaaagaact gaggaaactg aagaaccaag cttgacaatt
     5701 ggaagactaa tgcgttttcg agatatctag acccagcttt cttgtacaaa gtggttgata
     5761 acatctcgag ccataaagat ggttaattaa cccacccaag atctggcctc cgcgccgggt
     5821 tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg
     5881 gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata
     5941 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga
     6001 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct
     6061 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc
     6121 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt
     6181 ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg ggctttcgtg
     6241 gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg
     6301 ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct
     6361 gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg
     6421 tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag
     6481 ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca
     6541 ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg
     6601 ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc
     6661 tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc
     6721 gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc
     6781 tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc
     6841 ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt
     6901 gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt
     6961 gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacgaag
     7021 cttggtaccg agctcggatc tccaccccgt accggtcctg cagtcgaatt caccatgtct
     7081 agactggaca agagcaaagt cataaacgga gctctggaat tactcaatgg tgtcggtatc
     7141 gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac
     7201 tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg
     7261 catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac
     7321 gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc
     7381 acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag
     7441 caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg
     7501 ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc
     7561 accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg gcagggagcc
     7621 gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag
     7681 tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat gctcccagcc
     7741 gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga cgattttgac
     7801 cttgacatgc tccccgggta actaagtaag gatccgcggc cgcactagag gaattccgcc
     7861 cctctccccc ccccccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     7921 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     7981 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     8041 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     8101 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     8161 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     8221 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     8281 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     8341 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     8401 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct taccggtacg
     8461 cgtgatgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     8521 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     8581 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     8641 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     8701 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
     8761 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     8821 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     8881 tgcacttcgt ggccgaggag caggactgac acatctgtac aagtaaagcg gccgcgactc
     8941 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     9001 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttta gtccctccca
     9061 attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     9121 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     9181 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     9241 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     9301 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     9361 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     9421 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     9481 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     9541 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     9601 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     9661 cgcctccccg catcgatacc gtcgacctcg atcgagacct agaaaaacat ggagcaatca
     9721 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     9781 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     9841 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     9901 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     9961 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
    10021 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
    10081 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
    10141 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
    10201 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
    10261 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
    10321 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
    10381 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
    10441 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
    10501 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
    10561 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
    10621 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
    10681 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
    10741 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
    10801 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
    10861 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
    10921 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
    10981 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
    11041 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
    11101 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
    11161 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
    11221 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
    11281 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
    11341 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
    11401 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
    11461 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
    11521 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
    11581 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
    11641 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
    11701 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
    11761 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
    11821 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
    11881 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
    11941 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
    12001 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
    12061 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    12121 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    12181 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
    12241 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    12301 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    12361 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    12421 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    12481 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    12541 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    12601 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    12661 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    12721 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    12781 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    12841 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    12901 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    12961 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    13021 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    13081 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    13141 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    13201 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    13261 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    13321 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    13381 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    13441 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    13501 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    13561 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    13621 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    13681 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    13741 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    13801 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    13861 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    13921 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    13981 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    14041 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    14101 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    14161 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    14221 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    14281 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    14341 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgac
//
